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  4. Recombinant Human Forkhead box protein C1 (FOXC1)

Recombinant Human Forkhead box protein C1 (FOXC1)

  • 货号：
    CSB-YP618640HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP618640HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP618640HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP618640HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FOXC1
  • Uniprot No.：
  • 别名：
    ARA; FKH L7; FKHL 7; FKHL7; Forkhead (Drosophila) like 7; Forkhead box C1; Forkhead box protein C1; Forkhead drosophila homolog like 7; Forkhead like 7; Forkhead related activator 3; Forkhead related protein FKHL7; Forkhead related transcription factor 3; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; FOX C1; FOXC 1; Foxc1; FOXC1_HUMAN; FREAC 3; FREAC-3; FREAC3; IGDA; IHG 1; IHG1; IRID 1; IRID1; Iridogoniodysgenesis type 1; Myeloid factor delta
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-553
  • 氨基酸序列
    MQARYSVSSP NSLGVVPYLG GEQSYYRAAA AAAGGGYTAM PAPMSVYSHP AHAEQYPGGM ARAYGPYTPQ PQPKDMVKPP YSYIALITMA IQNAPDKKIT LNGIYQFIMD RFPFYRDNKQ GWQNSIRHNL SLNECFVKVP RDDKKPGKGS YWTLDPDSYN MFENGSFLRR RRRFKKKDAV KDKEEKDRLH LKEPPPPGRQ PPPAPPEQAD GNAPGPQPPP VRIQDIKTEN GTCPSPPQPL SPAAALGSGS AAAVPKIESP DSSSSSLSSG SSPPGSLPSA RPLSLDGADS APPPPAPSAP PPHHSQGFSV DNIMTSLRGS PQSAAAELSS GLLASAAASS RAGIAPPLAL GAYSPGQSSL YSSPCSQTSS AGSSGGGGGG AGAAGGAGGA GTYHCNLQAM SLYAAGERGG HLQGAPGGAG GSAVDDPLPD YSLPPVTSSS SSSLSHGGGG GGGGGGQEAG HHPAAHQGRL TSWYLNQAGG DLGHLASAAA AAAAAGYPGQ QQNFHSVREM FESQRIGLNN SPVNGNSSCQ MAFPSSQSLY RTSGAFVYDC SKF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA-binding transcriptional factor that plays a role in a broad range of cellular and developmental processes such as eye, bones, cardiovascular, kidney and skin development. Acts either as a transcriptional activator or repressor. Binds to the consensus binding site 5'-[G/C][A/T]AAA[T/C]AA[A/C]-3' in promoter of target genes. Upon DNA-binding, promotes DNA bending. Acts as a transcriptional coactivator. Stimulates Indian hedgehog (Ihh)-induced target gene expression mediated by the transcription factor GLI2, and hence regulates endochondral ossification. Acts also as a transcriptional coregulator by increasing DNA-binding capacity of GLI2 in breast cancer cells. Regulates FOXO1 through binding to a conserved element, 5'-GTAAACAAA-3' in its promoter region, implicating FOXC1 as an important regulator of cell viability and resistance to oxidative stress in the eye. Cooperates with transcription factor FOXC2 in regulating expression of genes that maintain podocyte integrity. Promotes cell growth inhibition by stopping the cell cycle in the G1 phase through TGFB1-mediated signals. Involved in epithelial-mesenchymal transition (EMT) induction by increasing cell proliferation, migration and invasion. Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression. Plays a role in the gene regulatory network essential for epidermal keratinocyte terminal differentiation. Essential developmental transcriptional factor required for mesoderm-derived tissues, such as the somites, skin, bone and cartilage. Positively regulates CXCL12 and stem cell factor expression in bone marrow mesenchymal progenitor cells, and hence plays a role in the development and maintenance of mesenchymal niches for haematopoietic stem and progenitor cells (HSPC). Plays a role in corneal transparency by preventing both blood vessel and lymphatic vessel growth during embryonic development in a VEGF-dependent manner. Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression. May function as a tumor suppressor.
  • 基因功能参考文献：
    1. This study demonstrates that FOXC1 induces cancer stem cells (CSCs)-like properties in non-small cell lung cancer (NSCLC) by promoting beta-catenin expression. The findings indicate that FOXC1 is a potential molecular target for anti-CSC-based therapies in NSCLC PMID: 30189871
    2. Foxc1 promoted cell proliferation by upregulation PI3K/AKT signaling, which was inflammation-dependent. PMID: 30060822
    3. Knockdown of FOXC1 markedly suppressed cell migration and invasion in vitro, and resulted in downregulation of phosphorylatedRACalpha serine/threonineprotein kinase, protooncogene cMyc and Bcell lymphoma 2. PMID: 29328384
    4. Results provide evidence that FOXC1 is not required for initiation of EMT events but rather participates in the specification of mesenchymal cell phenotype through regulation of FGF receptor switching from FGFR2-IIIb to FGFR1-IIIc in response to TGFb1-induced EMT. PMID: 28684636
    5. In regulating cervical cancers metastasis by targeting FOXC1. PMID: 28810526
    6. Forkhead box C1 protein (FOXC1) promotes melanoma cell function by regulating macrophage stimulating 1 receptor (MST1R) and activating MST1R/PI3K/AKT pathway. PMID: 27533251
    7. expression of FOXC1 in BRCA1 mutant cell lines correlates with sensitivity to olaparib. Whether this is due to rates of proliferation or another mechanism is yet to be explored, but this, and the specificity of FOXC1 in BRCA1-mutant tumors, suggests a possible role for FOXC1 as a marker for targeted therapy. PMID: 27708239
    8. novel EGFR-NF-kappaB-FOXC1 signaling axis that is critical for BLBC cell function PMID: 28629477
    9. Taken together, these data indicate that FOXC1 is a novel hypoxia-induced transcription factor and plays a critical role in tumor microenvironment-promoted lung cancer progression. PMID: 28435457
    10. Genomic analysis of blood and excised valve tissue showed down-regulation of FOXC1 but also FOXC2 expression in the diseased aortic valve. This allows us to speculate on the potential role of FOXC1 in aortic valve anomalies. PMID: 28657660
    11. Results showed a significant higher FOXC1 expression in estrogen receptor-negative breast cancer than that in estrogen receptor-positive. Its overexpression reduced expression of ERalpha and cellular responses to tamoxifen suggesting that FOXC1 regulated expression of ERalpha and affected sensitivity of tamoxifen treatment in breast cancer PMID: 28028927
    12. present work reveals that FOXC1 is an important regulator of exocytosis and establishes a new link between FOXC1 and MYOC-associated glaucoma PMID: 28575017
    13. Glaucoma prevalence and phenotype are characterized in a cohort of glaucoma patients and their family members with FOXC1 variants. PMID: 28513611
    14. FOXC1 and FOXC2 are essential regulators of lymphangiogenesis and may have roles in lymphatic-associated diseases PMID: 27214551
    15. This review will summarize current knowledge on the function and regulation of FOXC1 in tumor development and progression with a focus on basal-like breast cancer, as well as the implications of these new findings in cancer diagnosis and treatment. [review] PMID: 28288141
    16. A novel heterozygous FOXC1 variant segregated with the disease in a family with Axenfeld Rieger Syndrome. A novel homozygous variant in the FOXC1 gene segregated in a family with ARS and congenital glaucoma. PMID: 27463523
    17. Our findings suggested that FOXCUT expression contributed to the development and progression of nasopharyngeal carcinoma by targeting FOXC1 and that FOXCUT might be useful as a potential nasopharyngeal carcinoma biomarker and therapeutic target. PMID: 28635400
    18. this study defines FOXC1 as a regulator specific for KC terminal differentiation and establishes its potential position in the genetic regulatory network. PMID: 27907090
    19. FOXC1 is correlated with chemosensitivity to anthracycline and could be used as an indicator of chemosensitivity in sporadic triple-negative breast cancer PMID: 28493031
    20. Elevated expression of FOXC1 enhanced the invasion ability of BLCB cells in vitro. PMID: 27685901
    21. this report describes an Italian family in which four subjects share brachydactyly type E and a 3 Mb microduplication in region 6p25. The duplication involves the gene FOXC1, expressed during the osteoblast differentiation, which appears a potential candidate gene for brachydactyly. PMID: 28111183
    22. miR-495 acts as a tumour suppressor gene by targeting FOXC1 at the post-transcriptional level in endometrial cancer PMID: 26198045
    23. This report presents the clinical characteristics of a Belgian family with a 3.4 Mb deletion of chromosome 6p25, including the FOXC1 gene. PMID: 27242366
    24. data reveal the presence of translation regulatory sequences in the UTRs of FOXC1 and provide evidence for a possible role of rare FOXC1 variants as modifying factors of goniodysgenesis in primary congenital glaucoma. PMID: 26220699
    25. ARS is an autosomal dominant disorder with high penetration. It is primarily caused by a mutation of the pituitary homeobox 2 (PITX2) or forkhead box C1 (FOXC1) gene. Mutations in PITX2 on chromosome 4q25, or in FOXC1 at 6p25, have been identified in approximately 40% to 70% of patients with ARS PMID: 26240509
    26. These data suggest that deregulation of FOXC1 or its downstream genes play a major role in the pathogenesis of coarctation of the aorta in humans. PMID: 26643481
    27. Disruptions of enhancers near FOXC1 and GMDS may influence brain development. PMID: 26382291
    28. We report a novel FOXC1 mutation (p. R127L) in a three-generation family with three ARS and heart defect patients. PMID: 24914578
    29. FOXC1 directly binds Gli2 and potentiates Gli2 DNA-binding activity. PMID: 26565916
    30. This study shows that hsa-miR548-l regulates FOXC1 translation, contributing to better understand the fine regulation of the biological function of this transcription factor. PMID: 25809640
    31. A meta-analysis of the top SNPs identified three new associated loci in primary open angle glaucoma--TXNRD2, ATXN2, and FOXC1 PMID: 26752265
    32. These findings suggest that miR-4792 functions as a tumor suppressor in NPC development and progression by targeting FOXC1. PMID: 26585487
    33. Our data indicate that miR-138-5p may play an important role in regulating pancreatic cancer cell growth, possibly through targeting FOXC1 PMID: 25875420
    34. The results support that moderate and variable FOXC1 transactivation changes are associated with moderate goniodysgenesis, dominant glaucoma and remarkable phenotypic variability. PMID: 25786029
    35. Foxc1 is essential for progenitor cell development and maintenance of bone marrow niches for these cells upregulating CXCL12 and SCF expression and inhibiting of adipogenic processes in cell progenitors. (Review) PMID: 26049754
    36. In HCC cell lines, IL8 activates expression of FOXC1 via the phosphoinositide 3-kinase signaling to AKT and hypoxia-inducible factor 1alpha. FOXC1 expression leads to transactivation of CXCR1 and CCL2, promoting inflammation and invasivion/metastasis. PMID: 26065367
    37. Report lncRNA FOXC1 upstream transcript (FOXCUT) overexpressed in oral squamous cell carcinoma. PMID: 24889262
    38. We demonstrate that FOXC1 is required for epidermal growth factor-elicited cell proliferation, migration, and invasion. PMID: 25124473
    39. FOXC1 assay (IHC & qRT-PCR) accurately identified basal-like breast cancer (BLBC) in FFPE samples. FOXC1 expression also predicted the development of brain metastasis. PMID: 26041837
    40. we showed that a novel long non-coding RNA FOXCUT and its neighboring gene FOXC1 may function as a lncRNA-mRNA gene pair, which may represent a potential prognostic biomarker and therapeutic target for esophageal squamous cell carcinoma patients PMID: 25031703
    41. Genome-wide association analysis linked the FOXC1-interacting transcription factor PITX2 to cerebral small-vessel disease. PMID: 25250569
    42. suggest that reduced expression of miR-639 underscores the mechanism of TGFbeta-induced epithelial-mesenchymal transition in tongue squamous cell carcinoma by targeting FOXC1 and may serve as therapeutic targets in the process of metastasis PMID: 25130698
    43. Dysfunction of the stress-responsive FOXC1 transcription factor contributes to trabecular meshwork cell death and the earlier-onset glaucoma observed in Axenfeld-Rieger syndrome patients. PMID: 24556684
    44. Data indicate that among 31 thymus development-related genes, PBX1 copy number gain and FOXC1 copy number loss were presented in 43.0% and 39.5% of the tumors, respectively. PMID: 23444221
    45. A novel c.317delA mutation in FOXC1 in a Korean family with Axenfeld-Rieger syndrome, is reported. PMID: 23687430
    46. The data suggest existence of a complex regulatory pathway in the trabecular meshwork part of which includes interactions between FOXC1, miR-204, MEIS2, and ITGbeta1. PMID: 23541832
    47. High FOXC1 expression is associated with pancreatic ductal adenocarcinoma. PMID: 23242609
    48. High expression of FOXC1 is associated with non-small cell lung cancer patients. PMID: 23264086
    49. FOXC1 deletions were observed in four cases of anterior segment dysgenesis and related conditions. PMID: 22569110
    50. overexpression of FoxC1 in hepatpcellular carcinoma is a strong indicator of more-aggressive tumors and poor clinical outcome. PMID: 22911555

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  • 相关疾病：
    Axenfeld-Rieger syndrome 3 (RIEG3); Anterior segment dysgenesis 3 (ASGD3)
  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in keratinocytes of epidermis and hair follicle. Expressed strongly in microvascular invasion (MVI) formation, basal-like breast cancer (BLBC) and hepatocellular tumors. Expressed in breast cancers (at protein level). Expressed in hematopoietic
  • 数据库链接：

    HGNC: 3800

    OMIM: 601090

    KEGG: hsa:2296

    STRING: 9606.ENSP00000370256

    UniGene: Hs.348883