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  4. Recombinant Human Erythroid transcription factor (GATA1)

Recombinant Human Erythroid transcription factor (GATA1)

  • 货号：
    CSB-YP009274HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009274HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009274HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009274HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009274HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GATA1
  • Uniprot No.：
  • 别名：
    Anemia; X-linked; without thrombocytopenia; included; ERYF 1; Eryf1; Erythroid transcription factor; Erythrold transcription factor 1; GATA 1; GATA binding factor 1; GATA binding protein 1 (globin transcription factor 1); GATA binding protein 1; GATA-1; GATA-binding factor 1; GATA1; GATA1_HUMAN; GF 1; GF-1; GF1; Globin transcription factor 1; NF E1; NF E1 DNA binding protein; NF-E1 DNA-binding protein; NFE 1; NFE1 ; Nuclear factor erythroid 1; Transcription factor GATA1; XLANP; XLTDA; XLTT
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-413
  • 氨基酸序列
    MEFPGLGSLG TSEPLPQFVD PALVSSTPES GVFFPSGPEG LDAAASSTAP STATAAAAAL AYYRDAEAYR HSPVFQVYPL LNCMEGIPGG SPYAGWAYGK TGLYPASTVC PTREDSPPQA VEDLDGKGST SFLETLKTER LSPDLLTLGP ALPSSLPVPN SAYGGPDFSS TFFSPTGSPL NSAAYSSPKL RGTLPLPPCE ARECVNCGAT ATPLWRRDRT GHYLCNACGL YHKMNGQNRP LIRPKKRLIV SKRAGTQCTN CQTTTTTLWR RNASGDPVCN ACGLYYKLHQ VNRPLTMRKD GIQTRNRKAS GKGKKKRGSS LGGTGAAEGP AGGFMVVAGG SGSGNCGEVA SGLTLGPPGT AHLYQGLGPV VLSGPVSHLM PFPGPLLGSP TGSFPTGPMP PTTSTTVVAP LSS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.
  • 基因功能参考文献：
    1. Reduced GATA-1 could be responsible for the upregulation of IRF-3 in lung adenocarcinoma cells through binding with a specific domain of IRF-3 promoter. PMID: 28566697
    2. described the functional interaction between GATA1 and SEC23B genes in two patients with suspected congenital dyserythropoietic anemia type II PMID: 28550189
    3. Here, using zebrafish, murine, and human models, the s show that erythropoietin (EPO) signaling, together with the GATA1 transcriptional target, AKAP10, regulates heme biosynthesis during erythropoiesis at the outer mitochondrial membrane. PMID: 28553927
    4. expression of GATA1 effectively rescued maturation of Primary myelofibrosis megakaryocytes. PMID: 28240607
    5. GATA1 is an essential downstream target of SENP1 and that the differential expression and response of GATA1 and Bcl-xL are a key mechanism underlying chronic mountain sickness pathology. PMID: 27821551
    6. we herein show a long-distance regulatory region with GATA1 binding sites as being a strong enhancer for NBEAL2 expression. PMID: 28082341
    7. Single-nucleotide polymorphism in GATA1 gene is associated with non-Down syndrome transient proliferative megakaryoblastic disease. PMID: 27667142
    8. These findings indicate that erythroid specific activator GATA-1 acts at CTCF sites around the beta-globin locus to establish tissue-specific chromatin organization. PMID: 28161276
    9. Results show that GATA1 recognizes a single GATA motif or a composite of adjacent GATA motifs and exerts its diversified bindings. These binding configurations serve as one of the critical determinants of specific transcriptional regulation. PMID: 27215385
    10. Acquired and inherited GATA1 mutations contribute to Diamond-Blackfan anemia, acute megakaryoblastic leukemia, transient myeloproliferative disorder, and a group of related congenital dyserythropoietic anemias with thrombocytopenia. PMID: 28179280
    11. Our results suggest that GATAl and miR-363 were involved in the regulation of hematopoiesis via the HIF-1alpha pathway in K562 cells under hypoxic condition. PMID: 27485543
    12. analysis of GATA1 mutations in a cohort of Malaysian children with Down syndrome-associated myeloid disorder reveals distinctive genomic events PMID: 27353457
    13. trisomy 21 perturbed hematopoietic development through the enhanced production of early hematopoietic progenitors and the upregulation of mutated GATA1, resulting in the accelerated production of aberrantly differentiated cells. PMID: 27134169
    14. Data show that pyruvate kinase (PK) activity was decreased in the GATA1 hemizygous state and PKLR c.1284delA variant. PMID: 27342114
    15. GATA1 mutations were identified in consecutive Down syndrome patients with transient myeloproliferative disorder or acute leukemia. PMID: 26234152
    16. expression of GATA1 and SET7 was upregulated and positively correlated with VEGF expression and microvessel number in 80 breast cancer patients. GATA1 and SET7 are independent poor prognostic factors in breast cancer. PMID: 26848522
    17. Molecular cytogenetic analysis of leukemic blast cells indicated that increased blast cell status was caused by transient abnormal myelopoiesis with trisomy 21 and GATA1 mutation. PMID: 25711269
    18. deletion of P-sel disrupted megakaryocyte/neutrophil interactions in spleen, reduced TGF-beta content, and corrected the hematopoietic stem cells distribution that in Gata1(low) mice, as in primary myelofibrosis patients, is abnormally expanded in spleen. PMID: 26439305
    19. study provides insight into GATA1 transcriptional activity and may prove a useful resource for investigating the pathogenicity of noncoding variants in human erythroid disorders. PMID: 27044088
    20. The GATA-1-mediated inhibition of PU.1 gene transcription in human AML-erythroleukemias mediated through the URE represents important mechanism that contributes to PU.1 downregulation and leukemogenesis that is sensitive to DNA demethylation therapy PMID: 27010793
    21. findings provide insights into the clinically relevant in vivo function of the N-terminal domain of GATA1 in human hematopoiesis. PMID: 26713410
    22. Acute megakaryoblastic leukemia is associated with GATA-1 mutation, mimicking myeloproliferative disorders. PMID: 26205501
    23. GATA2-to-GATA1 switch is prevalent at dynamic enhancers and drives erythroid enhancer commissioning PMID: 26766440
    24. GATA1 and GATA2 are involved in clear cell renal cell carcinoma biology possibly affecting tumor development and aggressiveness. PMID: 25230694
    25. Congenital erythropoietic porphyria linked to GATA1-R216W mutation PMID: 25251786
    26. we uncovered a novel function of GATA1 in regulating Epithelial-mesenchymal transition PMID: 25726523
    27. Global transcriptome and chromatin occupancy analysis reveal the short isoform of GATA1 is deficient for erythroid specification and gene expression. PMID: 25682601
    28. EDAG forms complex with GATA1 and p300 and increases GATA1 acetylation and transcriptional activity by facilitating the interaction between GATA1 and p300 PMID: 24740910
    29. These results indicate that KLF1 plays a role in facilitating and/or stabilizing GATA-1 and TAL1 occupancy in the erythroid genes, contributing to the generation of active chromatin structure such as histone acetylation and chromatin looping PMID: 25528728
    30. Our case of transient leukemia without Down syndrome highlight the important role of trisomy 21 and GATA1 mutation in the development of transient neonatal leukemia. PMID: 24253371
    31. In erythroid cells, pull down experiments identified the presence of a novel complex formed by HDAC5, GATA1, EKLF and pERK which was instead undetectable in cells of the megakaryocytic lineage. PMID: 24594363
    32. Results demonstrate that expression of the hGATA1 gene is regulated through the chromatin architecture organized by 5'CTCF site-mediated intrachromosomal interactions in the hGATA1 locus. PMID: 25755285
    33. The results demonstrate that hGATA-1 and hGATA-2 expression in hippocampus is sufficient to cause depressive like behaviors. PMID: 25340772
    34. Lineage-specific GATA1 cofactor associations are essential for normal chromatin occupancy. PMID: 25621499
    35. Nkx2-5 binds to the Gata1 gene enhancer and represses the transcriptional activity of the Gata1 gene. PMID: 21464046
    36. a hypothesis is presented to explain that, in Down syndrome, the first mutational events, GATA1 somatic mutations, do not occur at random, but as a result of perturbed cell functions and specific over-expression of the GATA1 gene PMID: 24880866
    37. Data indicate that GATA1 transcription factor is downregulated in ribosomal protein S19 (RPS19)-deficient cells through upregulation of TNF-alpha and p38 MAPK. PMID: 25270909
    38. A functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p in erythropoiesis. PMID: 24608802
    39. Somatic GATA1 mutations appear to be pivotal in development of transient abnormal myelopoiesis and are proving to be markers of clonal identity in its evolution to acute megakaryoblastic leukemia in subjects with Down syndrome. [CASE STUDY; REVIEW] PMID: 25268193
    40. The high rate of GATA-1 gene mutations was confirmed in newborn infants with Down's Syndrome and transient abnormal myelopoiesis or acute megakaryoblastic leukemia PMID: 24196768
    41. Results report a forth family with clinical findings consistent with an association between GATA1 gene mutation and anemia black diamond. PMID: 24766296
    42. amplitude of a transcriptional signature of GATA1 target genes was globally and specifically reduced, indicating that the activity, but not the mRNA level, of GATA1 is decreased in patients with DBA PMID: 24952648
    43. PSTPIP2 dysregulation contributes to aberrant terminal differentiation in GATA-1-deficient megakaryocytes by activating LYN. PMID: 24407241
    44. High GATA1 expression is associated with hyperproliferation of eosinophil precursors in Down syndrome transient leukemia. PMID: 24336126
    45. Mutations in the GATA1 gene associated with leukemogenesis in newborns with Down syndrome. PMID: 24222239
    46. Loss of GATA-1 full length as a cause of Diamond-Blackfan anemia phenotype. PMID: 24453067
    47. Mitochondrial translation is dramatically affected after mGatA depletion, revealing an essential role for the GatCAB enzyme in the process of protein biosynthesis in mammalian mitochondria. PMID: 24579914
    48. Our results suggest that GATA1 exon 2 mutations occur late in trisomy 21 fetal hematopoiesis PMID: 24746204
    49. A role for GATA1 in chemotherapy resistance in non-Down syndrome acute megakaryocytic leukemia cells. PMID: 23874683
    50. multiple modes of the GATA1-MED1 axis may help to fine-tune GATA1 function during GATA1-mediated homeostasis events. PMID: 24245781

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  • 相关疾病：
    X-linked dyserythropoietic anemia and thrombocytopenia (XDAT); Thrombocytopenia with beta-thalassemia, X-linked (XLTT); Anemia without thrombocytopenia, X-linked (XLAWT)
  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Erythrocytes.
  • 数据库链接：

    HGNC: 4170

    OMIM: 300367

    KEGG: hsa:2623

    STRING: 9606.ENSP00000365858

    UniGene: Hs.765