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  4. Recombinant Human Endonuclease 8-like 1 (NEIL1)

Recombinant Human Endonuclease 8-like 1 (NEIL1)

  • 货号：
    CSB-EP846619HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    DNA (apurinic or apyrimidinic site) lyase Neil1; DNA glycosylase/AP lyase Neil 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease 8 like 1; Endonuclease 8-like 1; Endonuclease VIII; Endonuclease VIII like 1; Endonuclease VIII-like 1; FLJ22402; FPG1; hFPG1; NEH 1; NEH1; NEI 1; Nei endonuclease VIII like 1 (E. coli); Nei endonuclease VIII like 1; Nei homolog 1; Nei like 1; Nei like protein 1; Nei-like protein 1; NEI1; NEIL 1; Neil1; NEIL1_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    59.6kDa
  • 表达区域：
    2-390aa
  • 氨基酸序列
    PEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQEYQQFRENVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLGGKGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSAS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.
  • 基因功能参考文献：
    1. NEIL1 and homotetrameric mtSSB form a larger ternary complex in presence of DNA, however, the tetrameric form of mtSSB gets disrupted by NEIL1 in the absence of DNA as revealed by the formation of a smaller NEIL1-mtSSBmonomer complex. PMID: 29522991
    2. The major role of acetylable Lys residues in NEIL1 is to stabilize the formation of chromatin-bound repair complexes which protect cells from oxidative stress. PMID: 29698889
    3. The level of OGG1 expression was significantly reduced in bipolar patients compared to healthy individuals, whereas the two groups exhibited similar levels of NEIL1 expression. PMID: 29626765
    4. identified the c-Jun N-terminal kinase 1 (JNK1) as the kinase involved in the phosphorylation of NEIL1 PMID: 27518429
    5. NEIL1 (rs5745908) is associated with Behcet's disease. The genetic association in NEIL1 is a predicted splice donor variant that may introduce a deleterious intron retention and result in a noncoding transcript variant. PMID: 26662719
    6. cellular NEIL1 is regulated by the UPP mediated by the E3 ubiquitin ligases Mule and TRIM26, which plays a vital role in co-ordinating the cellular response to DNA damage. PMID: 27924031
    7. Nei Like DNA Glycosylase 1 (NEIL1) as a likely candidate gene due to its crucial role in B-cell activation and terminal differentiation. PMID: 28093361
    8. NEIL1 and NEIL2 cooperate with TDG during base excision: TDG occupies the abasic site and is displaced by NEILs, which further process the baseless sugar, thereby stimulating TDG-substrate turnover. PMID: 26751644
    9. first study to show that SNPs of genes involved in DNA repair, may modulate the risk of Depressive Disorder. PMID: 26074017
    10. Findings suggesting that DNA glycosylase NEIL1 c.C844T is a defective allele. PMID: 26095805
    11. NEIL1 forms a multiprotein complex with DNA replication proteins via its C-terminal domain, allowing recruitment at the replication fork. PMID: 26134572
    12. Results show that YB-1 interferes negatively with the AP site DNA cleavage activity of both APE1 and NEIL1 for ssDNA and bubble structures. PMID: 25605055
    13. Rad9 regulates base excision repair by controlling NEIL1 transcription. PMID: 25873625
    14. genome and cancer single nucleotide polymorphisms of the human NEIL1 DNA glycosylase PMID: 24382305
    15. The NEIL1 rs4462560 SNP may serve as a predictor of acute RIET and RP risk but not of overall survival. PMID: 24022861
    16. Prereplicative repair of oxidized bases in the human genome is mediated by NEIL1 DNA glycosylase together with replication proteins. PMID: 23898192
    17. s show that the intrinsically disordered C-terminal domain interacts with the folded domain in native NEIL1 containing 389 residues. PMID: 23542007
    18. NEIL1 recognizes specifically and distinctly interstrand crosslinks in DNA, and can obstruct the efficient removal of lethal crosslink adducts. PMID: 23508956
    19. binds to the BRCT domain of PARP-1 PMID: 23104860
    20. aberrant promoter methylation of NEIL1 in head and neck squamous cell carcinoma PMID: 22286769
    21. NEIL1 gene mutation may have a causative role in the development of type 2 diabetes in the Turkish population. PMID: 21985917
    22. we demonstrated that hNEIL1 and hNTH1 cleave Oz sites as efficiently as 5-hydroxyuracil sites. Thus, hNEIL1 and hNTH1 can repair Oz lesions PMID: 22465744
    23. the hnRNP-U protection of cells after oxidative stress is largely due to enhancement of NEIL1-mediated repair. PMID: 22902625
    24. Pro2 and Lys54 are involved in the AP lyase activity; Met81, Arg119 and Phe120 are essential for removal of 8-oxoG in duplex DNA PMID: 22858590
    25. the binding of these modified DNAs with the unedited and edited forms of human NEIL1 along with E. coli Endo III PMID: 22639086
    26. Structural characterization of viral ortholog of human DNA glycosylase NEIL1 bound to thymine glycol or 5-hydroxyuracil-containing DNA. PMID: 22170059
    27. Homozygosity mapping and exome sequencing in a consanguineous kindred identified MYO1E and NEIL1 as novel candidate genes for human autosomal recessive steroid-resistant nephrotic syndrome. PMID: 21697813
    28. These data suggest Neil1 may be a critical mediator of base excision repair of incorporated dUMP following thymidylate synthetase pathway inhibition. PMID: 21131780
    29. enzymological activity in peripheral leukocytes is increased in children with asthma bronchiale PMID: 19840299
    30. NEIL1 recoding site is a preferred editing site for the RNA editing adenosine deaminase ADAR1. PMID: 21068368
    31. WRN is the only human RecQ helicase that stimulates NEIL1 DNA glycosylase activity, and that this stimulation requires a double-stranded DNA substrate. PMID: 20346739
    32. Modulation of NEIL1's activity on single-stranded DNA substrate by RPA and PCNA support NEIL1's involvement in repairing the replicating genome. PMID: 20338831
    33. These results suggest that, in vivo, NEIL1 functions either at nucleosome-free regions (such as those near replication forks) or with cofactors that limit its non-specific binding to DNA. PMID: 20005182
    34. The results indicate that NEIL1 does not require a base opposite to identify and remove hydantoin lesions. PMID: 20099873
    35. Pyrimidine dimer repair enzyme located on the long arm of chromosome 15 that is frequently deleted in human cancers. PMID: 12509226
    36. NEIL1 and NEIL2 are preferentially involved in repair of lesions in DNA bubbles generated during transcription and/or replication PMID: 14522990
    37. hNEIL1 has a significant role in the repair of 5S-Tg in human cells. PMID: 14734554
    38. Overproduction, crystallization and preliminary crystallographic analysis of NEIL1 PMID: 15159582
    39. zincless finger appears to be required for NEIL1 activity, because mutating a very highly conserved arginine within this motif greatly reduces the glycosylase activity of the enzyme. PMID: 15232006
    40. Reduced expression of NEIL1 is associated with the pathogenesis of gastric cancers PMID: 15319300
    41. NEIL1 and OGG1, two DNA glycosylases which do not stably interact with each other, stimulate 8-oxoguanine repair by a collaboration that is possible because of higher abasic (AP) site affinity and stronger AP lyase activity of NEIL1 relative to OGG1. PMID: 15350146
    42. NEIL1 is a DNA glycosylase that excise 5-formyluracil, 5-hydroxymethyluracil and Thymine glycol in human cells. PMID: 15533839
    43. Oxidative stress-induced activation of NEIL1 appears to be involved in the feedback regulation of cellular repair activity needed to handle an increase in the level of oxidative base damage PMID: 16118226
    44. Nth1 released 5R,6S 2'-deoxyribonucleoside diastereoisomer (Tg2) much more rapidly than cis 5S,6R-deoxyribonucleoside diastereoisomer (Tg1) regardless of the opposing purine. Neil1 released Thymine glycol non-stereoselectively. PMID: 16446124
    45. A T434+2C mutation was found in familial colorectal cancer DNA suggesting a limited role for this gene in the devlopment of CRC. PMID: 17029639
    46. The damage specificity of human homologues of Endo III (hNTHl) and Endo VIII (hNEIL1 and hNEIL2) is compared to elucidate the repair role in cells. PMID: 17150535
    47. we found that although both SMUG1 and NEIL1 are able to excise 5-OHU lesions located in the proximity of the proximity of the 3'-end of a DNA SSB, NEIL1 is more efficient in the repair of these DNA lesions. PMID: 17348689
    48. Human NEIL1 DNA glycosylase activity is significantly stimulated by hHus1, hRad1, hRad9 separately and by the 9-1-1 complex. PMID: 17395641
    49. The ability of Neil1 to recognize a variety of pyrimidine lesions is connected to the flexible binding pocket of the enzyme and the common chemical features of lesions that contain a pyrimidine ring. PMID: 17432829
    50. Centrosomal localization of hNEIL1 was observed when mitotic HeLa cells were immunostained with hNEIL1 antibodies PMID: 17556049

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Chromosome. Note=During mitosis, associates with centrosomes and condensed chromatin.
  • 蛋白家族：
    FPG family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 18448

    OMIM: 608844

    KEGG: hsa:79661

    STRING: 9606.ENSP00000347170

    UniGene: Hs.512732