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  4. Recombinant Human ERO1-like protein beta (ERO1LB)

Recombinant Human ERO1-like protein beta (ERO1LB)

  • 货号：
    CSB-YP774834HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP774834HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP774834HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP774834HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP774834HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Endoplasmic oxidoreductin-1-like protein B; Endoplasmic reticulum oxidoreductin-1-like protein B; ERO1; S. cerevisiae; homolog of; beta; ERO1-L-beta; ERO1-like beta (S. cerevisiae); ERO1-like protein beta; ERO1B_HUMAN; Ero1lb; Oxidoreductin-1-L-beta
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    34-467
  • 氨基酸序列
    QVTGVLD DCLCDIDSID NFNTYKIFPK IKKLQERDYF RYYKVNLKRP CPFWAEDGHC SIKDCHVEPC PESKIPVGIK AGHSNKYLKM ANNTKELEDC EQANKLGAIN STLSNQSKEA FIDWARYDDS RDHFCELDDE RSPAAQYVDL LLNPERYTGY KGTSAWRVWN SIYEENCFKP RSVYRPLNPL APSRGEDDGE SFYTWLEGLC LEKRVFYKLI SGLHASINLH LCANYLLEET WGKPSWGPNI KEFKHRFDPV ETKGEGPRRL KNLYFLYLIE LRALSKVAPY FERSIVDLYT GNAEEDADTK TLLLNIFQDT KSFPMHFDEK SMFAGDKKGA KSLKEEFRLH FKNISRIMDC VGCDKCRLWG KLQTQGLGTA LKILFSEKEI QKLPENSPSK GFQLTRQEIV ALLNAFGRLS TSIRDLQNFK VLLQHSR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2 (50% of P4HB reoxidation rate), as well as PDIA3, PDIA4, PDIA6 and NXNDC12 (<10%). Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. May be involved in oxidative proinsulin folding in pancreatic cells, hence may play a role in glucose homeostasis.
  • 基因功能参考文献：
    1. This peptide, Ac-VDTTD-AFC, was efficiently cleaved by purified caspase-2 and auto-activating caspase-2 in mammalian cells, and exhibited better selectivity for caspase-2 relative to caspase-3 than reagents that are currently available. PMID: 27919037
    2. GJB2 and ERO1LB are implicated in pancreatic cancer progression and can be used to predict patient survival PMID: 28177904
    3. upregulated in pancreatic neuroendocrine tumors PMID: 26765469
    4. In the present work, it was shown that recombinant human Ero1beta is twice as active as Ero1alpha in enzymatic assays. PMID: 21091435
    5. glutathione limits Ero1-dependent oxidation in the endoplasmic reticulum PMID: 15161913
    6. analysis of Ero1beta tissue distribution and dimerization PMID: 16012172
    7. Ero1alpha and Ero1beta are retained in the endoplasmic reticulum by interactions with PDI and ERp44 PMID: 16677073
    8. the Ero FAD binding domain is critical for conformational stability, allowing Ero proteins to withstand stress conditions that cause client proteins to misfold PMID: 16822866
    9. The lack of correlation between changes in SAT adiponectin gene and protein expression and its circulating levels suggests that adipose tissue synthesis and release of adiponectin are highly regulated pathways. PMID: 18996753

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side. Note=The association with ERP44 may be essential for its retention in the endoplasmic reticulum.
  • 蛋白家族：
    EROs family
  • 组织特异性：
    Highly expressed in the digestive tract, including the duodenum and lower digestive tract. In the stomach, highly expressed in enzyme-producing chief cells (at protein level). In the pancreas, expressed in islets of Langerhans and, at lower levels, in enz
  • 数据库链接：

    HGNC: 14355

    OMIM: 615437

    KEGG: hsa:56605

    STRING: 9606.ENSP00000346635

    UniGene: Hs.558519