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  4. Recombinant Human E3 ubiquitin-protein ligase XIAP (XIAP)

Recombinant Human E3 ubiquitin-protein ligase XIAP (XIAP)

  • 货号：
    CSB-EP026193HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    AP 13; API3; Apoptosis Inhibitor 3; Baculoviral IAP repeat containing 4; Baculoviral IAP Repeat Containing Protein 4; Baculoviral IAP repeat-containing protein 4; BIRC 4; BIRC4; E3 ubiquitin-protein ligase XIAP; hIAP-3; hIAP3; HILP; IAP 3; IAP like protein; IAP-3; IAP-like protein; IAP3; ILP 1; ILP; ILP1; Inhibitor of apoptosis protein 3; Inhibitor of Apoptosis X Linked; Mammalian IAP Homologue A; MIHA; X linked IAP; X linked inhibitor of apoptosis; X linked inhibitor of apoptosis E3 ubiquitin protein ligase; X linked inhibitor of apoptosis protein; X-linked IAP; x-linked inhibitor of apopotosis proteins; X-linked inhibitor of apoptosis protein; Xiap; XIAP_HUMAN; XLP2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    72.7kDa
  • 表达区域：
    1-497aa
  • 氨基酸序列
    MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, CASP3, CASP7, CASP8, CASP9, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS and BIRC5/survivin. Ubiquitinion of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitinion of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinationg COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Acts as an important regulator of innate immune signaling via regulation of Nodlike receptors (NLRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program.
  • 基因功能参考文献：
    1. carnosic acid induces parkin by enhancing the ubiquitination of ARTS, leading to induction of XIAP. PMID: 28224479
    2. Study identified the promoter region of miR-320a and verified three transcription factors, ATF2, ELK1, and YY1 to be responsible for inducing miR-320a expression under ionizing radiation conditions. Also, novel miR-320a target gene, XIAP, was identified. These findings suggest that miR-320a enhances the radiosensitivity of cancer cells. PMID: 30066913
    3. Embelin promoted apoptosis via XIAP and PI3K/Akt signaling pathway. XIAP inhibitor Embelin inducing apoptosis could cause osteosarcoma cell death and inhibit its invasion. PMID: 30249882
    4. prohibitin 1 regulates tumor cell apoptosis via the interaction with X-linked inhibitor of apoptosis protein PMID: 27025967
    5. To expand the computational strategy designed when studying XIAP, we have applied the molecular modeling tools to a list of 140 variants seen in CFTR associated with cystic fibrosis, and a list of undiagnosed variants in 17 different genes. Graphical abstract XIAP in Caspase 3 and NOD2 signaling pathways. PMID: 28204942
    6. A 3'UTR reporter assay confirms miR-124-3p to be a bona fide regulator of XIAP in CHO-K1 cells. This method demonstrates a useful approach to finding miRNA candidates for CHO cell engineering without competing for the cellular translational machinery. PMID: 28976632
    7. The CASP-9 and -3/7 inhibitors XIAP 242Delta and Delta230 were prepared using the pGEX expression system and biochemically characterized. HtrA2 specifically binds to the BIR2 and BIR3 of XIAP at a 1:1 molecular ratio. in vitro cell-free CASP-9 and -3/7 Activation-apoptosis assays have demonstrated that these purified XIAP proteins dramatically inhibit CASP-9 and -3/7 action. PMID: 29631059
    8. that XIAP rs8371 and rs9856 are associated with susceptibility to esophageal squamous cell carcinoma PMID: 29037837
    9. In a systematic analysis of variants in 23 primary immuno-deficiencies-associated genes, study confirmed the association of variants in XIAP with Crohn's disease. PMID: 29501442
    10. These results reveal that RPS3 upregulates XIAP independently of the NF-kappaB pathway in human breast cancer cells PMID: 29048653
    11. downregulation of XIAP expression could enhance the sensitivity of RCC cells to apoptosis, and the basal expression of XIAP during apoptosis is stable. PMID: 29115633
    12. XIAP promotes ubiquitylation and degradation of Bcl-2. PMID: 29020630
    13. XIAP gene silencing would strengthen the radiosensitivity of esophageal cancer cells in vivo and in vitro, which provides a novel target for the treatment of esophageal cancer. PMID: 28821565
    14. Findings of the study confirm that L-THP resulted in p53 independent apoptosis via down-regulating XIAP protein by inhibiting MDM2 associated with proteasome-dependent pathway and increased sensitivity of EU-4 cells against doxorubicin. PMID: 28721806
    15. miR-23a induces trophoblast cell apoptosis by inhibiting XIAP, which may contribute to Preeclampsia. PMID: 28772220
    16. These data suggest that XIAP may be playing an important role in the pathogenesis of breast cancer and can be therapeutically targeted either alone or in combination with PI3-kinase inhibition to induce efficient apoptosis in breast cancer cells. PMID: 28893228
    17. loss of XIAP enhances filopodia formation in a Cdc42-dependent manner and this phenomenon phenocopies EGF stimulation. Further, XIAP depletion promotes lung colonization of tumor cells in mice in a Cdc42-dependent manner. These observations shed molecular insights into ubiquitin-dependent regulation of Cdc42 and that of actin cytoskeleton. PMID: 28661476
    18. XIAP role in the cisplatin resistance in colon cancer PMID: 28667026
    19. Here, the s show that transcriptional regulator interacting with the PHD-bromodomain 1 (TRIP-Br1, Sertad1), a newly identified protein with poorly characterized functions, acts as an adaptor that bridges the interaction of multiple Adenylyl cyclase isoforms with X-linked inhibitor of apoptosis protein (XIAP), a RING-domain E3 ubiquitin ligase. PMID: 28656888
    20. In gastroenteropancreatic neuroendocrine neoplasms survivin and XIAP regulate cell proliferation and apoptosis functioning as possible prognostic biomarkers and therapeutic targets. PMID: 28039474
    21. A temporal succession of transcriptional and post-transcriptional regulatory mechanisms of the miR~23a~24-2~27a cluster, enabling a dynamic stress response and assuring cellular survival after radiation exposure by stabilizing XIAP. PMID: 28851536
    22. the RING domain of XIAP has a role in promoting miR-4295 transcription, which reduces p63alpha translation and enhances urothelial transformation PMID: 27447744
    23. These effects of metformin were accompanied by robust reductions in the protein levels of XIAP, a negative regulator of TRAIL-induced apoptosis. Silencing XIAP in TNBC cells mimicked the TRAIL-sensitizing effects of metformin. Metformin also enhanced the antitumor effects of TRAIL in a metastatic murine TNBC model PMID: 28324269
    24. There were higher expressions of XIAP (p = 0.0032) and Bcl-2 (p = 0.0351) in the glioblastoma samples compared to the control samples of normal brain. These results raise the question of whether Bcl-2 and XIAP genes can be responsible for the inhibition of programmed cell death in glioblastomas. PMID: 29236891
    25. These results show that significantly increased levels of FOXO3, IRF4, and xIAP mRNA in Chinese HIV-1-infected patients. PMID: 27841661
    26. the aim of this review is to emphasize to clinicians that XIAP deficiency should be considered in patients suspected to have Crohn disease with early onset or an aggravated clinical course resistant to conventional therapy, and that appropriate treatment strategies are available. PMID: 27416006
    27. uncovered a novel function of BIR domain of XIAP in regulating the EGFR translation PMID: 28057023
    28. our study revealed a novel microRNA signaling pathway involved in cholangiocarcinoma and suggests that manipulation of the miR-410/XIAP pathway could have a therapeutic potential for cholangiocarcinoma PMID: 27259577
    29. Inhibition of BET bromodomain-dependent XIAP and FLIP expression sensitizes KRAS-mutated non-small cell lung cancer to pro-apoptotic agents. PMID: 27607580
    30. XIAP interacts with FAF1 and blocks FAF1-induced cell death. PMID: 28414080
    31. The study identifies an unanticipated role for the X-linked inhibitor of apoptosis (XIAP) protein as a regulator of Lys63-linked polyubiquitination of HIF1alpha. PMID: 28666324
    32. The s find that primary human aggressive B-cell lymphoma samples exhibit high USP9X expression that correlate with XIAP overexpression. PMID: 27317434
    33. Findings reveal important new insights into how XIAP 3'UTR works, suggesting that the non-coding XIAP 3'UTR serves as a competitor for miRNA binding and subsequently inactivates miRNA functions, by which XIAP 3'UTR frees the target mRNAs from being repressed. PMID: 28186968
    34. XIAP is stable during mitotic arrest, but its function is controlled through phosphorylation by the mitotic kinase CDK1-cyclin-B1 at S40. PMID: 27927753
    35. our findings suggest that X-linked inhibitor of apoptosis protein 3'-untranslated region serves as a competitive endogenous RNA for HMGA2 to activate hepatocellular carcinoma progression by arresting endogenous let-7a-5p. PMID: 28691642
    36. In chronic myeloid leukemia cells, EZH2 modulates epigenetic changes at DNA methylated regions encoding miR-219 and downregulates the level of miR-219. Downregulation of miR-219 results in upregulation of XIAP and supports the survival of leukemia cells. PMID: 27070757
    37. IFNgamma positively affects NOD2-mediated signaling in human conventional dendritic cells, in a manner considerably dependent on XIAP and partially dependent on mTOR. PMID: 26521691
    38. miR-137 expression is inversely correlated with the level of XIAP protein in both ovarian cancer tissues and cell lines. these data suggest that multiple miRNAs can regulate XIAP via its 3'UTR. miR-137 can sensitise ovarian cancer cells to cisplatin-induced apoptosis, providing new insight into overcoming drug resistance in ovarian cancer PMID: 27875524
    39. results of this work support the role of XIAP in mediating NOD2 signaling while reconciling the role of X-linked lymphoproliferative syndrome 2 and very-early-onset inflammatory bowel disease XIAP mutations in inflammatory cell death and provide a set of tools and framework to rapidly test newly discovered XIAP variants. PMID: 28404814
    40. Using recombinant proteins, this study investigated the influence of survivin on the inhibition of caspase-9 by XIAP in vitro. With a fluorescence-based assay for the apoptosome-stimulated activity of caspase-9, the study shows that survivin has no effect on the inhibition of caspase-9 by XIAP, neither in the presence nor in the absence of Smac. PMID: 27865841
    41. Study found a negative correlation between Smac and XIAP at the level of protein but not mRNA in non-small cell lung carcinoma (NSCLC) patients. Overexpressed XIAP could degrade through ubiquitination, the mature Smac inhibiting NSCLC apoptosis. PMID: 27498621
    42. Combined triple actions of the TRAIL, the IAPs inhibitor, AT406, and the c-FLIP expression inhibitor, rocaglamide (ART), markedly improve TRAIL-induced apoptotic effects in most solid cancer cell lines through the activation of an extrinsic apoptosis pathway PMID: 27210546
    43. we identified X-linked inhibitor of apoptosis protein (XIAP) as a direct target of miR-509-3p. PMID: 27697095
    44. MiR-874 inhibits growth, increases apoptosis and enhances chemosensitivity in CRC cells by targeting XIAP. PMID: 27221209
    45. Data show that X-linked inhibitor of apoptosis protein (XIAP) mRNA and protein expression increased, whereas XIAP-associated factor 1 (XAF1) mRNA and protein expression decreased with the growth of radiation dose and exposure time. PMID: 27048285
    46. We demonstrated that, while depletion of XIAP attenuates cell transformation, elevated USP11 significantly promotes the tumor colony formation through stabilization of XIAP. PMID: 28040451
    47. High XIAP expression is associated with ovarian cancer. PMID: 26779627
    48. miR-24 plays an important role in regulating the cell survival of periodontal ligament cells through targeting XIAP. PMID: 27188727
    49. Herein, CEUS imaging during cancer treatment played an important role in evaluating the therapeutic effects of XIAP siRNA ultrasound-responsive microbubble are a promising theranostic system for cancer gene therapy. PMID: 26733262
    50. herefore targeting XIAP and NF-kappaB pathways simultaneously can be investigated in more detail to make use of embelin and celastrol as a combination therapy of cancer PMID: 27072230

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  • 相关疾病：
    Lymphoproliferative syndrome, X-linked, 2 (XLP2)
  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=TLE3 promotes its nuclear localization.
  • 蛋白家族：
    IAP family
  • 组织特异性：
    Ubiquitous, except peripheral blood leukocytes.
  • 数据库链接：

    HGNC: 592

    OMIM: 300079

    KEGG: hsa:331

    STRING: 9606.ENSP00000347858

    UniGene: Hs.356076