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  4. Recombinant Human E3 ubiquitin-protein ligase LRSAM1 (LRSAM1)

Recombinant Human E3 ubiquitin-protein ligase LRSAM1 (LRSAM1)

  • 货号：
    CSB-YP751010HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP751010HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP751010HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP751010HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP751010HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    LRSAM1
  • Uniprot No.：
  • 别名：
    CMT2P; E3 ubiquitin protein ligase LRSAM1; E3 ubiquitin-protein ligase LRSAM1; EC 6.3.2.-; FLJ31641 ; hTAL; Leucine rich repeat and sterile alpha motif containing 1; Leucine rich repeat and sterile alpha motif containing protein 1; Leucine-rich repeat and sterile alpha motif-containing protein 1; LRSAM1; LRSM1_HUMAN; OTTHUMP00000022174 ; OTTHUMP00000022175 ; RIFLE ; TAL ; Tsg101 associated ligase; Tsg101-associated ligase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-723
  • 氨基酸序列
    MPLFFRKRKP SEEARKRLEY QMCLAKEAGA DDILDISKCE LSEIPFGAFA TCKVLQKKVL IVHTNHLTSL LPKSCSLLSL ATIKVLDLHD NQLTALPDDL GQLTALQVLN VERNQLMQLP RSIGNLTQLQ TLNVKDNKLK ELPDTVGELR SLRTLNISGN EIQRLPQMLA HVRTLEMLSL DASAMVYPPR EVCGAGTAAI LQFLCKESGL EYYPPSQYLL PILEQDGIEN SRDSPDGPTD RFSREELEWQ NRFSDYEKRK EQKMLEKLEF ERRLELGQRE HTQLLQQSSS QKDEILQTVK EEQSRLEQGL SEHQRHLNAE RQRLQEQLKQ TEQNISSRIQ KLLQDNQRQK KSSEILKSLE NERIRMEQLM SITQEETESL RRRDVASAMQ QMLTESCKNR LIQMAYESQR QNLVQQACSS MAEMDERFQQ ILSWQQMDQN KAISQILQES AMQKAAFEAL QVKKDLMHRQ IRSQIKLIET ELLQLTQLEL KRKSLDTESL QEMISEQRWA LSSLLQQLLK EKQQREEELR EILTELEAKS ETRQENYWLI QYQRLLNQKP LSLKLQEEGM ERQLVALLEE LSAEHYLPIF AHHRLSLDLL SQMSPGDLAK VGVSEAGLQH EILRRVQELL DAARIQPELK PPMGEVVTPT APQEPPESVR PSAPPAELEV QASECVVCLE REAQMIFLNC GHVCCCQQCC QPLRTCPLCR QDIAQRLRIY HSS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos. Bacterial recognition protein that defends the cytoplasm from invasive pathogens. Localizes to several intracellular bacterial pathogens and generates the bacteria-associated ubiquitin signal leading to autophagy-mediated intracellular bacteria degradation (xenophagy).
  • 基因功能参考文献：
    1. We identified a novel LRSAM1 missense mutation (c.2120C > T, p.Pro707Leu) mapping to the RING domain. The identified missense mutation, as well as of another recently reported pathogenic missense mutation (c.2081G > A, p.Cys694Tyr), revealed that in vitro ubiquitylation activity was largely abrogated. PMID: 28335037
    2. findings suggest that the mutant LRSAM1 may aberrantly affect the formation of transcription machinery. PMID: 27615052
    3. findings demonstrate that the isolated genetic entity Charcot-Marie-Tooth type 2G is caused by a missense mutation in LRSAM1. PMID: 27686364
    4. LRSAM1 exhibited self-association in vitro and in vivo. The study found the self-association of LRSAM1 promotes intermolecular ubiquitination and proved a potential N-terminal ubiquitination. PMID: 28189685
    5. disruption of the C-terminal RING domain confers dominant negative properties to LRSAM1 PMID: 24894446
    6. Our data further confirms that LRSAM1 mutations are associated with CMT2 of AD inheritance. PMID: 22781092
    7. s identify LRSAM1 as the E3 ligase responsible for anti-Salmonella autophagy-associated ubiquitination. PMID: 23245322
    8. homozygous mutation in LRSAM1 was proposed as a strong candidate for the disease in a family with recessive axonal polyneuropathy PMID: 22012984
    9. LRSAM1 as a component of the antibacterial autophagic response. PMID: 20616063
    10. LRSAM1 is a strong candidate for the causal gene for the Charcot-Marie-Tooth disease. PMID: 20865121
    11. Results suggest that RIFLE represents a novel signaling protein that mediates components of the Wnt/wingless signaling pathway and cell adhesion in PC12 cells [RIFLE protein]. PMID: 14635195
    12. Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane PMID: 15256501
    13. Tal polyubiquitinates lysine residues in the C-terminus of uncomplexed Tsg101, resulting in proteasomal degradation. PMID: 18077552

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  • 相关疾病：
    Charcot-Marie-Tooth disease 2P (CMT2P)
  • 亚细胞定位：
    Cytoplasm.
  • 组织特异性：
    Highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue.
  • 数据库链接：

    HGNC: 25135

    OMIM: 610933

    KEGG: hsa:90678

    STRING: 9606.ENSP00000300417

    UniGene: Hs.495188