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  4. Recombinant Human E3 ubiquitin/ISG15 ligase TRIM25 (TRIM25)

Recombinant Human E3 ubiquitin/ISG15 ligase TRIM25 (TRIM25)

  • 货号：
    CSB-YP617909HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP617909HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP617909HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP617909HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP617909HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    E3 ubiquitin/ISG15 ligase TRIM25; EFP; Estrogen responsive finger protein; Estrogen-responsive finger protein; RING finger protein 147; RNF 147; RNF147; TRI25; TRI25_HUMAN; TRIM 25; Trim25; Tripartite motif containing 25; Tripartite motif containing protein 25; Tripartite motif protein TRIM25; Tripartite motif-containing protein 25; Tripartite motifcontaining25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Z147; Zinc finger protein 147 (estrogen responsive finger protein); Zinc finger protein 147; Zinc finger protein-147; ZNF 147; ZNF147
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-630
  • 氨基酸序列
    MAELCPLAEE LSCSICLEPF KEPVTTPCGH NFCGSCLNET WAVQGSPYLC PQCRAVYQAR PQLHKNTVLC NVVEQFLQAD LAREPPADVW TPPARASAPS PNAQVACDHC LKEAAVKTCL VCMASFCQEH LQPHFDSPAF QDHPLQPPVR DLLRRKCSQH NRLREFFCPE HSECICHICL VEHKTCSPAS LSQASADLEA TLRHKLTVMY SQINGASRAL DDVRNRQQDV RMTANRKVEQ LQQEYTEMKA LLDASETTST RKIKEEEKRV NSKFDTIYQI LLKKKSEIQT LKEEIEQSLT KRDEFEFLEK ASKLRGISTK PVYIPEVELN HKLIKGIHQS TIDLKNELKQ CIGRLQEPTP SSGDPGEHDP ASTHKSTRPV KKVSKEEKKS KKPPPVPALP SKLPTFGAPE QLVDLKQAGL EAAAKATSSH PNSTSLKAKV LETFLAKSRP ELLEYYIKVI LDYNTAHNKV ALSECYTVAS VAEMPQNYRP HPQRFTYCSQ VLGLHCYKKG IHYWEVELQK NNFCGVGICY GSMNRQGPES RLGRNSASWC VEWFNTKISA WHNNVEKTLP STKATRVGVL LNCDHGFVIF FAVADKVHLM YKFRVDFTEA LYPAFWVFSA GATLSICSPK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3. Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks.
  • 基因功能参考文献：
    1. formation of unanchored K63-linked poly-ubiquitin chains is unchanged by NS1 binding, indicating that RING dimerisation of TRIM25 is not affected by NS1 PMID: 29739942
    2. The RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain, which we postulate to be a novel RNA-binding domain. PMID: 29117863
    3. TRIM25 inhibits hepatocellular carcinoma progression by targeting MTA1. PMID: 28861931
    4. TRIM25 is a key determinant of breast cancer metastasis. PMID: 28813674
    5. V interaction with TRIM25 and RIG-I prevents TRIM25-mediated ubiquitination of RIG-I and disrupts downstream RIG-I signaling to the mitochondrial antiviral signaling protein. PMID: 29321315
    6. HPV E6 oncoprotein antagonizes the activation of the cytoplasmic innate immune sensor RIG-I by targeting its upstream regulatory enzymes TRIM25 and USP15. We further show that the RIG-I signaling cascade is important for an antiviral innate immune response to HPV16 infection PMID: 29263274
    7. this study shows thatTRIM25 targets ERG for degradation in prostate cancer PMID: 27626314
    8. TRIM25 actively participates in higher-order assembly of the RIG-I signalosome. PMID: 27425606
    9. Our results indicate that TRIM25 is associated with cisplatin resistance and 14-3-3sigma-MDM2-p53 signaling pathway is involved in this process, suggesting targeting TRIM25 may be a potential strategy for the reversal of cisplatin resistance. PMID: 28867193
    10. expression of TRIM25 might be critical for lung cancer cell migration, proliferation as well as doxorubicin resistance. PMID: 26113559
    11. Disturbed p53-MDM2 feedback loop contributing to the pathogenesis of thoracic aortic dissection may be linked to TRIM25 overexpression. PMID: 27903487
    12. the data uncover TRIM25 as a bona fide zinc-finger antiviral protein cofactor that leads to increased zinc-finger antiviral protein modification enhancing its translational inhibition activity. PMID: 28060952
    13. Studies showed that 70% of breast cancer patients were positive for Trim25 expression. PMID: 27528750
    14. we provide a detailed characterization of the TRIM ligases TRIM25 and TRIM32 and show how their oligomeric state is linked to catalytic activity PMID: 27154206
    15. The severe acute respiratory syndrome coronavirus N protein was found to bind to the SPRY domain of the tripartite motif protein 25 (TRIM25) E3 ubiquitin ligase, thereby interfering with the association between TRIM25 and retinoic acid-inducible gene I (RIG-I) and inhibiting TRIM25-mediated RIG-I ubiquitination and activation. PMID: 28148787
    16. TRIM25 is required for Zinc finger antiviral protein optimal binding to target mRNA. PMID: 28202764
    17. TRIM25 may exert its function through TGF-beta pathway. PMID: 26754079
    18. TRIM25 plays an additional role in RIG-I/MDA5 signaling other than RIG-I ubiquitination via activation of NF-kappaB. PMID: 26299329
    19. Data show that TRIM25 enhances p53 and Mdm2 abundance by preventing their proteasomal degradation. At the same time, TRIM25 inhibits p53's transcriptional activity and dampens the response to DNA damage. PMID: 25728675
    20. The PR-2B DENV-2 produced increased levels of subgenomic flavivirus RNA (sfRNA) relative to genomic RNA during replication. PR-2B sfRNA showed sequence dependent binding to and prevention of tripartite motif 25 (TRIM25) deubiquitylation, which is critical for sustained and amplified retinoic acid-inducible gene 1 (RIG-I)-induced type I interferon expression. PMID: 26138103
    21. Trim25 binds to the conserved terminal loop (CTL) of pre-let-7 and activates TuT4, allowing for more efficient Lin28a-mediated uridylation. PMID: 25457611
    22. Our data uncovers a previously unknown functional link between gp78 and TRIM25 and provides mechanistic insight into gp78-mediated protein ubiquitylation. PMID: 24810856
    23. Data indicate ubiquitin-specific protease 15 (USP15) as a critical regulator of the tripartite motif protein 25 (TRIM25)- and RNA sensor retinoic acid-inducible gene-I (RIG-I)-mediated antiviral immune response PMID: 24399297
    24. In controls, RNF125 is the highest expressed gene, whereas in HIV infection progressors, RIG-I is either the highest expressed gene or is expressed similarly to RNF125 and TRIM25. PMID: 24131985
    25. Study reports that RLR activation triggers MAVS ubiquitination on lysine 7 and 10 by the E3 ubiquitin ligase TRIM25 and marks it for proteasomal degradation concomitantly with downstream signaling. PMID: 22626058
    26. EFP mediates estrogen-induced ATBF1 protein degradation in breast cancer cells. PMID: 22452784
    27. Studies indicate that the splice variant of RIG-I encodes a protein that lacks the first CARD of RIG-I, and the variant RIG-I protein is not ubiquitinated by TRIM25. PMID: 21890623
    28. RING-IBR-RING E3 ligase domains of HOIL-1L and HOIP bind and induce proteasomal degradation of TRIM25, whereas the NZF domain of HOIL-1L competes with TRIM25 for RIG-I binding PMID: 21292167
    29. Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains. PMID: 21266548
    30. The regulation of Efp and bFGF expression during endometrial carcinogenesis suggests their potential utility as a prognostic biomarker. PMID: 20127022
    31. Efp controls breast cancer growth through ubiquitin-dependent proteolysis of 14-3-3sigma. PMID: 12943693
    32. Results indicate that estrogen-responsive finger protein is widely expressed and may play important roles in various human tissues, possibly through estrogen receptors. PMID: 15130519
    33. Over expression of Estrogen-responsive finger protein is associated with tumor progression of breast carcinoma PMID: 16144914
    34. 14-3-3 sigma expression inversely correlated with estrogen receptor alpha, progesterone receptor and estrogen-responsive finger protein, and positively correlated with myometrial invasion and lymph node metastasis in endometrial adenocarcinoma PMID: 16271083
    35. Efp is an IFN-responsive gene that potentially mediates IFN actions, involved in ISGylation and ubiquitination of proteins including Efp itself. PMID: 17069755
    36. Our results indicate that EFP functions as a cofactor for ERalpha-mediated transcription. PMID: 17418098
    37. role of the intact tandem CARD for TRIM25-mediated RIG-I activation; TRIM25 binds the RIG-I first CARD and subsequently ubiquitinates its second CARD PMID: 18948594
    38. estradiol and tamoxifen may regulate the growth of endometrial carcinoma cells by stimulating VEGF production through Efp. PMID: 19148513
    39. Viral nonstructural protein 1 inhibits activation of RIG-I by binding to TRIM25. PMID: 19454344
    40. NS1 inhibits TRIM25-mediated RIG-I ubiquitination, thereby suppressing RIG-I signal transduction. PMID: 19454348

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, Stress granule. Nucleus.
  • 组织特异性：
    Expressed in breast tumors (at protein level). Ubiquitous.
  • 数据库链接：

    HGNC: 12932

    OMIM: 600453

    KEGG: hsa:7706

    STRING: 9606.ENSP00000323889

    UniGene: Hs.528952