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  4. Recombinant Human Dual specificity protein phosphatase 5 (DUSP5)

Recombinant Human Dual specificity protein phosphatase 5 (DUSP5)

  • 货号：
    CSB-YP620976HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP620976HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP620976HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP620976HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP620976HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dual specificity phosphatase 5; Dual specificity protein phosphatase 5; Dual specificity protein phosphatase hVH 3; Dual specificity protein phosphatase hVH3; DUS5_HUMAN; DUSP 5; DUSP; DUSP5; HVH 3; HVH3; Serine/threonine specific protein phosphatase; VH 3; VH1 like phosphatase 3; VH3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-384
  • 氨基酸序列
    MKVTSLDGRQ LRKMLRKEAA ARCVVLDCRP YLAFAASNVR GSLNVNLNSV VLRRARGGAV SARYVLPDEA ARARLLQEGG GGVAAVVVLD QGSRHWQKLR EESAARVVLT SLLACLPAGP RVYFLKGGYE TFYSEYPECC VDVKPISQEK IESERALISQ CGKPVVNVSY RPAYDQGGPV EILPFLYLGS AYHASKCEFL ANLHITALLN VSRRTSEACA THLHYKWIPV EDSHTADISS HFQEAIDFID CVREKGGKVL VHCEAGISRS PTICMAYLMK TKQFRLKEAF DYIKQRRSMV SPNFGFMGQL LQYESEILPS TPNPQPPSCQ GEAAGSSLIG HLQTLSPDMQ GAYCTFPASV LAPVPTHSTV SELSRSPVAT ATSC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dual specificity protein phosphatase; active with phosphotyrosine, phosphoserine and phosphothreonine residues. The highest relative activity is toward ERK1.
  • 基因功能参考文献：
    1. Methylation the DUSP5 gene promoter can serve as an additional means of identifying CIMP-high colorectal cancers. PMID: 29379130
    2. DUSP5 expression in skeletal muscle was ninefold higher immediately after exercise and returned to pre-exercise level within 2 h. PMID: 28989118
    3. Findings indicate that zinc-finger protein X-linked (ZFX) promotes colorectal cancer (CRC) progression by suppressing dual specificity phosphatase 5 (DUSP5) expression and suggest that ZFX is a prognostic biomarker and potentially useful therapeutic target in stage II/III CRC patients. PMID: 26967242
    4. Results show that DUSP5 and DUSP6 mRNA are overexpressed in human PTCs, especially in BRAFV600E mutated papillary thyroid carcinomas (PTCs), and positively control cell migration and invasion. PMID: 28910386
    5. role of the secondary binding site in assembling the DUSP5-pERK pre-reactive complex was further demonstrated by molecular dynamics simulations that showed that the remote C197-C219 disulfide linkage controls the structure of the secondary binding pocket based on its redox state (i.e., disulfide/dithiol) and, in turn, the enzymatic activity of DUSP5 PMID: 27739308
    6. The DUSP5 S147P protein is hypoactive compared to the DUSP5 wild-type protein. PMID: 25519881
    7. Data indicate that cells from Burkitt's lymphoma, leukemia, neuroblastoma and Ewing sarcoma showed a higher dual-specificity phosphatase 5 pseudogene 1 (DUSP5P1)/dual specificity phosphatase 5 (DUSP5 ratio than normal cells. PMID: 24651368
    8. DUSP5 methylation may serve as a prognostic marker for gastric cancer (GC), but this requires validation in a larger set of GC samples. PMID: 23402999
    9. Studies indicate that dual-specificity MAP kinase phosphatases (MKPs) DUSP6/MKP-3 and DUSP5 are localized in the cytoplasm and nuclear compartments. PMID: 22812510
    10. DUSP5 and DUSP6 selectively control ERK pathway activity and proliferation. PMID: 20806045
    11. the differential up-regulation of MKP3 by Ets2 and of DUSP5 by c-Jun may converge in similar functional roles for these MAP kinase phosphatases in the growth arrest versus proliferation decisions of breast cancer cells PMID: 20554528
    12. In FD-Fms cells, overexpression of human DUSP5 increased M-CSF-dependent proliferation & decreased differentiation. Overexpression in multipotent EGER-Fms cells increased M-CSF-induced proliferation & caused granulocytic, not macrophage, differentiation. PMID: 19801501
    13. DUSP5 is a direct target of p53. PMID: 12944906
    14. DUSP5 inactivates ERK2 and causes both nuclear translocation and sequestration of inactive ERK2. PMID: 15713638
    15. the crystal structure of the catalytic domain of DUSP5 in an active conformation. PMID: 17078075
    16. proper regulation of DUSP5 activity is critical for normal immune system development, IL-2 actions, and tolerance PMID: 18430737
    17. mutations in dusp-5 and snrk-1 have been identified in affected tissues of patients with vascular anomalies, implicating the Snrk-1-Dusp-5 signaling pathway in human disease. PMID: 18927432

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
  • 数据库链接：

    HGNC: 3071

    OMIM: 603069

    KEGG: hsa:1847

    STRING: 9606.ENSP00000358596

    UniGene: Hs.2128