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  4. Recombinant Human Double-strand-break repair protein rad21 homolog (RAD21)

Recombinant Human Double-strand-break repair protein rad21 homolog (RAD21)

  • 货号：
    CSB-YP019257HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019257HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019257HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019257HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019257HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CDLS4; Double-strand-break repair protein rad21 homolog; hHR21; HR21; HRAD21; KIAA0078; MCD1; Nuclear matrix protein 1; NXP-1; NXP1; Protein involved in DNA double-strand break repair; RAD21; RAD21 homolog (S. pombe); RAD21 homolog; RAD21_HUMAN; Scc1; SCC1 homolog
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-631
  • 氨基酸序列
    MFYAHFVLSK RGPLAKIWLA AHWDKKLTKA HVFECNLESS VESIISPKVK MALRTSGHLL LGVVRIYHRK AKYLLADCNE AFIKIKMAFR PGVVDLPEEN REAAYNAITL PEEFHDFDQP LPDLDDIDVA QQFSLNQSRV EEITMREEVG NISILQENDF GDFGMDDREI MREGSAFEDD DMLVSTTTSN LLLESEQSTS NLNEKINHLE YEDQYKDDNF GEGNDGGILD DKLISNNDGG IFDDPPALSE AGVMLPEQPA HDDMDEDDNV SMGGPDSPDS VDPVEPMPTM TDQTTLVPNE EEAFALEPID ITVKETKAKR KRKLIVDSVK ELDSKTIRAQ LSDYSDIVTT LDLAPPTKKL MMWKETGGVE KLFSLPAQPL WNNRLLKLFT RCLTPLVPED LRKRRKGGEA DNLDEFLKEF ENPEVPREDQ QQQHQQRDVI DEPIIEEPSR LQESVMEASR TNIDESAMPP PPPQGVKRKA GQIDPEPVMP PQQVEQMEIP PVELPPEEPP NICQLIPELE LLPEKEKEKE KEKEDDEEEE DEDASGGDQD QEERRWNKRT QQMLHGLQRA LAKTGAESIS LLELCRNTNR KQAAAKFYSF LVLKKQQAIE LTQEEPYSDI IATPGPRFHI I
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions. The cohesin complex may also play a role in spindle pole assembly during mitosis. In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome. May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter. May play a role in embryonic gut development, possibly through the regulation of enteric neuron development.; May promote apoptosis.
  • 基因功能参考文献：
    1. evidence for a possible pathogenetic role of RAD21 promoter methylation in the development of chronic lymphocytic leukemia. PMID: 29587287
    2. With tissue microarrays of hepatocellular carcinoma (HCC) patients, we determined the prognostic values of the core genes in the network and found that RAD21, CDK1, and HDAC2 expression levels were negatively associated with overall survival for HCC patients. PMID: 28434945
    3. This study together with previous reports suggests incomplete penetrance associated with RAD21 variants and these individuals may therefore be underdiagnosed. PMID: 27882533
    4. Data indicate that RAD21 plays an important role in cellular senescence breast cancer cells, mainly through RB1 pathway activation via c-Myc downregulation. PMID: 26529363
    5. Reduced RAD21 destabilizes high-level gene amplification by disrupting pre-replication complex bindings in human cancers with chromosomal instability. PMID: 26420833
    6. found well-positioned CTCF and RAD21 peaks approximately 60-80 bp upstream of the TSS in the unidirectional genes. The peak heights were related to the amount of antisense transcription. Results provide insights into the distribution of histone modifications at promoters and suggest a novel role of CTCF and cohesin as regulators of transcriptional direction. PMID: 25881024
    7. Studied the role of looping in large-scale (supra Mb) folding of human chromosomes by knocking down the gene that codes for CTCF and the one coding for Rad21, an essential subunit of cohesin. PMID: 25299688
    8. Elevated RAD21 expression tracks with reactivation of L1 expression in human sporadic colorectal cancer. PMID: 25464844
    9. Some patients with chronic intestinal pseudo-obstruction carry mutations in RAD21 that disrupt the ability of its product to regulate genes such as RUNX1 and APOB. PMID: 25575569
    10. CTCF and Rad21 act as host cell restriction factors for Kaposi's sarcoma-associated herpesvirus (KSHV) lytic replication by modulating viral gene transcription. PMID: 24415941
    11. cohesin's proposed DNA exit gate is formed by interactions between Scc1 and the coiled-coil region of Smc3. PMID: 25414306
    12. Cross-sectional deep-sequencing analysis for clonal hierarchy demonstrated STAG2, SMC3, and RAD21 mutations to be ancestral in 18%, 18%, and 47% of cases, respectively, and each expanded to clonal dominance concordant with disease transformation PMID: 25006131
    13. RAD21 expression in CRC is associated with aggressive disease especially in KRAS mutant tumours and resistance to chemoradiotherapy. PMID: 24548858
    14. This study reports the first intragenic deletion and frameshift mutations identified in RAD21 in two patients presenting with atypical Cornelia de Lange syndrome. PMID: 24378232
    15. -RAD21 and EIF3H, both on chromosome 8q23, CHRAC1 on chromosome 8q24.3 and TANC2 on chromosome 17q23-were confirmed to be driver genes regulating the proliferation/survival of clonogenic breast cancer cells PMID: 24148822
    16. RAD21 expression confers poor prognosis and resistance to chemotherapy in high grade luminal, basal and HER2 breast cancers. PMID: 21255398
    17. Rad21 binds to SA proteins through two SA-binding motifs on Rad21. PMID: 23874961
    18. ASURA specifically binds to chromatin when Scc1 is associated with chromatin. PMID: 23548868
    19. RAD21 expression is associated with a poorer prognosis in BRCA2, but not in BRCA1 breast cancers. PMID: 22537934
    20. show that the Smc5/6 subunit Mms21 sumoylates multiple lysines of the cohesin subunit Scc1 PMID: 22751501
    21. RAD21 mutations cause a human cohesinopathy PMID: 22633399
    22. Rad21 cleavage by calpain-1 promotes separation of chromosome arms, which coincides with a calcium-induced partial loss of cohesin at several chromosomal loci. PMID: 21876002
    23. hRAD21 was significantly differently expressed in cells maintaining telomere length through a telomerase-independent mechanism, termed the alternative lengthening of telomeres (ALT). PMID: 20364118
    24. Rad21 protein depletion causes centrosome defects in interphase and prophase of mitosis. PMID: 20404533
    25. Rad21 protein are cleaved by separase are required for the localization of Rad21 to the centrosome. PMID: 20404544
    26. As compared to the telomerase group, the alternative lengthening of telomeres group presents higher expression of hRad21, thinner tumor invasion depth, and higher survival rate. PMID: 18197499
    27. Caspase proteolysis of the cohesin component RAD21 promotes apoptosis PMID: 11875078
    28. RAD21 subunit of the cohesin complex directly interacts with ATPase subunit SNF2; mapping of RAD21, SNF2 and Mi2 binding sites reveals specific association of these three proteins with DNA elements containing Alu sequences PMID: 12198550
    29. Scc1 is essential for the association of kinetochores with microtubules. PMID: 12200439
    30. plays a role in apoptosis, and its cleavage during apoptosis may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway PMID: 12417729
    31. RAD21 shows increased expression in clinical prostate carcinomas and is also amplified in 30-40% of xenografts and hormone-refractory tumors. PMID: 14603436
    32. Phosphorylation of SA2 is essential for cohesin dissociation during prophase and prometaphase, but is not required for cohesin cleavage by separase. PMID: 15737063
    33. RAD21 suppression enhances the antitumor activity of chemotherapeutic agents acting via induction of DNA damage PMID: 15767545
    34. The RAD21 gene was closely related to the invasion and metastasis of cancer cells. PMID: 16416296
    35. These data argue that the regulation of the IL-3 and the GM-CSF promoters depends on the positions of their enhancers relative to the conserved CTCF/cohesin-binding sites. PMID: 19158269
    36. The depletion of either CTCF or RAD21 disrupts the chromatin loop structure, together with significant changes in the APO expression. PMID: 19322193
    37. Ectopic expression of Rad21 repressed CTCF-regulated transcription of KSHV lytic genes. PMID: 19369356

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  • 相关疾病：
    Cornelia de Lange syndrome 4 (CDLS4)
  • 亚细胞定位：
    [Double-strand-break repair protein rad21 homolog]: Nucleus. Nucleus matrix. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole.; [64-kDa C-terminal product]: Cytoplasm, cytosol. Nucleus.
  • 蛋白家族：
    Rad21 family
  • 组织特异性：
    Expressed in the gut (at protein level).
  • 数据库链接：

    HGNC: 9811

    OMIM: 606462

    KEGG: hsa:5885

    STRING: 9606.ENSP00000297338

    UniGene: Hs.81848