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  4. Recombinant Human DnaJ homolog subfamily C member 3 (DNAJC3)

Recombinant Human DnaJ homolog subfamily C member 3 (DNAJC3)

  • 货号：
    CSB-YP615670HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP615670HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP615670HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP615670HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP615670HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DnaJ (Hsp40) homolog subfamily C member 3; DnaJ homolog subfamily C member 3; DNAJC3; DNJC3_HUMAN; double-stranded RNA-activated protein kinase inhibitor; Endoplasmic reticulum DnaJ protein 6; ERdj6; HP58; Interferon induced; double stranded RNA activated protein kinase inhibitor; Interferon-induced; P58; p58 ipk; P58IPK; PRKRI; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58; Protein kinase interferon inducible double stranded RNA dependent inhibitor
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    32-504
  • 氨基酸序列
    GVNADVEKH LELGKKLLAA GQLADALSQF HAAVDGDPDN YIAYYRRATV FLAMGKSKAA LPDLTKVIQL KMDFTAARLQ RGHLLLKQGK LDEAEDDFKK VLKSNPSENE EKEAQSQLIK SDEMQRLRSQ ALNAFGSGDY TAAIAFLDKI LEVCVWDAEL RELRAECFIK EGEPRKAISD LKAASKLKND NTEAFYKIST LYYQLGDHEL SLSEVRECLK LDQDHKRCFA HYKQVKKLNK LIESAEELIR DGRYTDATSK YESVMKTEPS IAEYTVRSKE RICHCFSKDE KPVEAIRVCS EVLQMEPDNV NALKDRAEAY LIEEMYDEAI QDYETAQEHN ENDQQIREGL EKAQRLLKQS QKRDYYKILG VKRNAKKQEI IKAYRKLALQ WHPDNFQNEE EKKKAEKKFI DIAAAKEVLS DPEMRKKFDD GEDPLDAESQ QGGGGNPFHR SWNSWQGFNP FSSGGPFRFK FHFN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
  • 基因功能参考文献：
    1. DNAJC3 p.H238N is likely to be a variant causing diabetes. PMID: 29767246
    2. p58IPK is a general inhibitor of the eIF2alpha kinases in that it also interacts with GCN2. Thus forced overexpression of cytoplasmic p58 delays eIF2alpha phosphorylation, suppresses GCN2 phosphorylation and prolongs protein synthesis PMID: 25329545
    3. Loss-of-function DNAJC3 mutations lead to a monogenic, recessive form of diabetes mellitus in humans. PMID: 25466870
    4. p58(IPK) suppresses coxsackievirus B3-induced apoptosis through selective activation of PI3K/Akt pathway that requires activation of ATF6a and subsequently upregulates mitofusin 2. PMID: 24134518
    5. interplay between p38 phosphorylation and p58(IPK) upregulation has key roles in modulating ERp29-induced cell-growth arrest and survival PMID: 22064321
    6. the crystal structure of human P58(IPK) PMID: 21799829
    7. Coxsackievirus B3 infection induces apoptosis through downregulation of p58IPK and activation of CHOP and SREBP1. PMID: 20554776
    8. Spleen cells from transgenic mice overexpressing (Hu)PKR were studied and compared to those from wild-type mice after drug treatment. Dact-treated spleen cells were resistant to apoptosis; ConA-treated cells had decreased cell division. PMID: 12034040
    9. P58(IPK) is an important component of a negative feedback loop used by the cell to inhibit eIF2alpha signaling, and thus attenuate the unfolded protein response. PMID: 12601012
    10. The study demonstrates that P58(IPK) inhibits endoplasmic reticulum (ER) stress and plays an important role in maintaining balance and stability of the ER in human retinal capillary endothelial cells. PMID: 18568130

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  • 相关疾病：
    Ataxia, combined cerebellar and peripheral, with hearing loss and diabetes mellitus (ACPHD)
  • 亚细胞定位：
    Endoplasmic reticulum.
  • 组织特异性：
    Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
  • 数据库链接：

    HGNC: 9439

    OMIM: 601184

    KEGG: hsa:5611

    STRING: 9606.ENSP00000365991

    UniGene: Hs.59214