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  4. Recombinant Human Dickkopf-related protein 3 (DKK3)

Recombinant Human Dickkopf-related protein 3 (DKK3)

  • 中文名称：
    人DKK3重组蛋白
  • 货号：
    CSB-YP890660HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人DKK3重组蛋白
  • 货号：
    CSB-EP890660HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人DKK3重组蛋白
  • 货号：
    CSB-EP890660HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人DKK3重组蛋白
  • 货号：
    CSB-BP890660HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人DKK3重组蛋白
  • 货号：
    CSB-MP890660HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DKK3
  • Uniprot No.：
  • 别名：
    Dickkopf 3; Dickkopf 3 homolog (Xenopus laevis); Dickkopf 3 homolog; Dickkopf homolog 3; Dickkopf related protein 3; Dickkopf related protein 3 Precursor; Dickkopf WNT signaling pathway inhibitor 3; Dickkopf; Xenopus; homolog of; 3; Dickkopf-3; Dickkopf-related protein 3; Dickkopf3; DKK 3; Dkk-3; DKK3; DKK3_HUMAN; hDkk 3; hDkk-3; hDkk3; Regulated in glioma; REIC; RIG; RIG-like 5-6; RIG-like 7-1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-350
  • 氨基酸序列
    APAPTATSA PVKPGPALSY PQEEATLNEM FREVEELMED TQHKLRSAVE EMEAEEAAAK ASSEVNLANL PPSYHNETNT DTKVGNNTIH VHREIHKITN NQTGQMVFSE TVITSVGDEE GRRSHECIID EDCGPSMYCQ FASFQYTCQP CRGQRMLCTR DSECCGDQLC VWGHCTKMAT RGSNGTICDN QRDCQPGLCC AFQRGLLFPV CTPLPVEGEL CHDPASRLLD LITWELEPDG ALDRCPCASG LLCQPHSHSL VYVCKPTFVG SRDQDGEILL PREVPDEYEV GSFMEEVRQE LEDLERSLTE EMALREPAAA AAALLGGEEI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • 基因功能参考文献：
    1. These findings suggested that the down-regulated REIC expression might be involved in lung carcinogenesis due to its promoter methylation PMID: 29341059
    2. the results indicate that TNFalpha downregulates REIC/Dkk3 expression in normal skin keratinocytes. PMID: 29512745
    3. These results show that miR-25-3p has intracellular and extracellular oncogenic functions as well as clinicopathological relevance in osteosarcoma, indicating its potential as a novel diagnostic and therapeutic tool for the clinical management of this disease. PMID: 29674765
    4. DKK3 might contribute to cellular proliferation, invasion, migration, and tumor cell survival in head and neck/oral squamous cell carcinoma cells through a mechanism other than the canonical Wnt signaling pathway, which might be attributed to PI3K-Akt signaling PMID: 28470144
    5. this study demonstrated the anti-glioma effect of Ad-SGE-REIC, which may represent a promising strategy for the treatment of malignant glioma. PMID: 27625116
    6. DKK3 is a novel and promising molecular target for keloid treatment. PMID: 28458155
    7. This report demonstrates that GATA4 promotes oncogenesis by inhibiting miR125b-dependent suppression of DKK3 expression. This GATA4/miR125b/DKK3 axis may be a major regulator of growth, migration, invasion, and survival in hepatoma cells. PMID: 27788486
    8. dickkopf-3 (Dkk-3) is a tumor suppressor gene that is downregulated in various cancers. PMID: 28168749
    9. Study shows a reduced expression of DKK3 in adrenocortical carcinoma potentially contributing to adrenal dedifferentiation and/or progression of malignancy. PMID: 28249601
    10. Low DKK3 expression is associated with gastric cancer. PMID: 28218426
    11. studies decoding the underlying molecular mechanisms of DKK3-mediated effects may help to identify novel targeted therapies for this clinically highly relevant breast cancer subtype PMID: 27467270
    12. REIC/Dkk-3 could be involved early in squamous cell carcinoma development and have inhibitory effect on the development of SCC PMID: 27354306
    13. Administration of DKK3 protein and peripheral blood mononuclear cell transplantation synergistically enhanced anticancer immunological effects against pancreatic cancer cells. PMID: 26643412
    14. Study showed that DKK3 promotes cell survival during oxidative stress by suppressing XDH expression, thereby abrogating excess reactive oxygen species accumulation and subsequent apoptosis. PMID: 28299863
    15. High DKK3 expression is associated with malignant progression of oral submucous fibrosis. PMID: 27959428
    16. The data indicated that Dkk3 has a novel role in modulating macropinocytotic and autophagic pathways, a distinct function other than a Wnt antagonist. PMID: 27827955
    17. we observed Dickkopf-related protein 3 (DKK3) as a direct target of miR-25 in vitro. Upregulation of DKK3 partially attenuated the oncogenic effect of miR-25 on melanoma cells. Ectopic expression of miR-25 in melanoma cells induced beta-catenin accumulation in nuclear and inhibited TCF4 (T cell factor 4) activity, as well as the expression of c-Myc and Cyclin D1. PMID: 27801786
    18. in situ Ad-REIC((REIC)/Dkk-3 gene) gene therapy is expected to realize a new-generation cancer vaccine via anticancer immune activation with DC and tumor antigen-specific CTL expansion. PMID: 26836118
    19. Loss of DKK3 expression in dysplastic/advanced stage of oral submucous fibrosis may imply a high risk of progression to oral cancer. PMID: 27349317
    20. Dkk3 is upregulated in osteoarthritis and may have a protective effect on cartilage integrity by preventing proteoglycan loss and helping to restore OA-relevant signaling pathway activity. PMID: 26687825
    21. A decreased risk of lung cancer was found for the genotype combination of DKK3 and sFRP4. PMID: 26457595
    22. Data indicate that Dickkopf-related protein 3 (DKK3) improved gemcitabine therapeutic effect through inducing apoptosis and regulating beta-catenin/epithelial-mesenchymal transition (EMT) signalling in pancreatic cancer cell. PMID: 26395974
    23. these data suggest that downregulation of tubular beta-catenin signaling induced by Dkk-3 has a detrimental role in chronic proteinuria, partially through the increase in apoptosis. PMID: 27010856
    24. Down-regulation of Dkk3 is associated with recurrence in Endometrioid Adenocarcinoma. PMID: 26397159
    25. beta2M-mediated VEGFR-2/Akt/mTOR phosphorylation and tumor angiogenesis was significantly suppressed by over-expression of DKK-3. PMID: 26278164
    26. Observations highlight a role for Dkk-3 in modulating TGF-beta/MMP signals in the prostate and prostate cancer. PMID: 26503968
    27. first report documenting that DKK3 polymorphisms are not only associated with prostate cancer but also related to prostate cancer-related factors. PMID: 26689513
    28. REIC/Dkk-3 is a naturally circulating serum protein, the upregulation REIC/Dkk-3 protein expression could be a promising option for cancer therapy PMID: 25823913
    29. we propose that Dickkopf-related protein-3 (Dkk-3) might be a novel Amyloid-beta (Abeta) associated protein PMID: 26119087
    30. Dkk-3 has a tumor-suppressive function and a proapoptotic effect, inducing apoptosis through mitochondrial and Fas death receptor pathways in human mucinous ovarian cancer MCAS cells. PMID: 25514350
    31. Epigenetic downregulation of DKK3 leads to docetaxel resistance in human nonsmall cell lung cancer (NSCLC) cells. PMID: 25760729
    32. reactivation of Dkk3 enhances the chemosensitivity to cisplatin in cisplatinresistant lung adenocarcinoma cell lines PMID: 25573172
    33. Suggest that DKK3 may be important in mediating invasion in esophageal adenocarcinoma. PMID: 26093488
    34. DKK3 downregulation across all MB subgroups may be due to epigenetic mechanisms, in particular, through chromatin condensation. PMID: 23567267
    35. Loss of DKK3 protein expression was significantly associated with poor survival in patients with gastric cancer and was strongly correlated with the TNM stage. PMID: 25604390
    36. Loss of DKK3 expression is associated with glioblastoma. PMID: 25301448
    37. The results suggested that the lack of affinity between human DKK3 and human LRP5/6 results from: i) insertion of the 7 amino acids, and ii) P258 in human DKK3. PMID: 24743782
    38. Loss of REIC expression is associated withnon-small cell lung cancer. PMID: 24498395
    39. data indicates that miR-92b directly regulate cell proliferation and apoptosis by targeting DKK3 and act as prognostic factors for glioma patients PMID: 24325785
    40. Dkk3 levels corrected to platelet count showed higher levels in polycythemia vera patients than normal controls. Elevated Dkk3c level could possibly correlate to platelet activation in polycythemia vera patients and increased Dkk3 release. PMID: 24309205
    41. DKK3 gene is a potential tumor suppressor gene in thyroid cancer, and aberrant promoter methylation is an important mechanism for its downregulation, which may play a role in the tumorigenesis and aggressiveness of papillary thyroid carcinoma. PMID: 23702469
    42. elevated Dkk-3 in the stroma of the diseased prostate presumably regulates stromal remodeling by enhancing proliferation and differentiation of stromal cells and contributing to the angiogenic switch observed in BPH and prostate cancer. PMID: 23765731
    43. These data demonstrated that Dkk-3 expression in OSCC was different than that in adenocarcinomas. Dkk-3 may possess an oncogenic function that is independent of Wnt signaling. PMID: 23354949
    44. We developed a protocol to generate smooth muscle cells from pluripotent stem cells through a dickkopf 3 signaling pathway, useful for generating tissue-engineered vessels. PMID: 23529184
    45. Data identifies Dkk-3 and SMAD4 as potential target genes of miR-183 in prostate cancer. PMID: 23538390
    46. Decreased DKK3 expression was associated with advanced International Federation of Gynecology and Obstetrics clinical stages and was predictive of lower disease-free survival in patients with cervical squamous cell carcinoma. PMID: 23154266
    47. aberrant expression of REIC might play some role in growth and lymph node metastasis of gastric carcinoma by suppressing growth, migration and invasion and/or by inducing apoptosis PMID: 22421143
    48. Dkk3 gene expression is frequently downregulated in endometrial cancer, and is associated with poor prognostic clinicopathologic markers. Results identify a role for Dkk3 as a tumor suppressor in EC, affecting both proliferation and invasiveness. PMID: 22555103
    49. Dkk-3 overexpression induced apoptosis in human colon cancer possibly through the mitochondrial pathway. Dkk-3 may be involved in the Wnt/beta-catenin signaling pathways in colon cancer. PMID: 22529687
    50. Five bladder cancer cell lines among six cell lines examined were resistant to adenovirus-Dkk-3. PMID: 22076283

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Dickkopf family
  • 组织特异性：
    Highest expression in heart, brain, and spinal cord.
  • 数据库链接：

    HGNC: 2893

    OMIM: 605416

    KEGG: hsa:27122

    STRING: 9606.ENSP00000314910

    UniGene: Hs.292156