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  4. Recombinant Human Delta (24)-sterol reductase (DHCR24), partial

Recombinant Human Delta (24)-sterol reductase (DHCR24), partial

  • 货号：
    CSB-YP618005HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP618005HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP618005HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP618005HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP618005HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DHCR24
  • Uniprot No.：
  • 别名：
    24 dehydrocholesterol reductase ; 24-dehydrocholesterol reductase; 3 beta hydroxysterol delta 24 reductase; 3-beta-hydroxysterol delta-24-reductase; DCE; Delta(24)-sterol reductase; Desmosterol to cholesterol enzyme; DHC24_HUMAN; Dhcr24; Diminuto dwarf1 homolog ; Diminuto/dwarf1 homolog; KIAA0018; Nbla03646; Seladin-1; SELADIN1; Selective AD indicator 1; Selective Alzheimer's Disease Indicator 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. In addition to its cholesterol-synthesizing activity, can protect cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis.
  • 基因功能参考文献：
    1. The crucial role of the insulin/STAT3/DHCR24/PGR axis in the progression of endometrial carcinoma. PMID: 28112250
    2. DHCR24 might be a therapeutic target for patients with bladder cancer PMID: 30278482
    3. Mutation in DHCR24 gene cause desmosterolosis presenting with multiple congenital anomalies with increased level of the cholesterol precursor desmosterol while disrupting development of cholesterol, impacting embryogenesis. PMID: 29175559
    4. Study observed decreased methylation at the DHCR24 locus in offspring of women with active pregnancy eating disorders (ED) and increased methylation at the LGALS2 locus in offspring of women with past ED compared to controls. PMID: 29093763
    5. the expanding database of post-translational modifications will be a valuable resource for mapping the topology of membrane-associated proteins, such as DHCR24, that is, flagging cytosolic residues accessible to modifying enzymes such as kinases and ubiquitin ligases. PMID: 27919032
    6. DHCR24 auto-antibody represents a potential noninvasive biomarker for hepatitis C virus-related liver disease and may facilitate the diagnosis of PIVKA-II and AFP-negative hepatocellular carcinoma. PMID: 26288822
    7. In vitro experiments showed that DHCR24 overexpression induced tumor proliferation. PMID: 24562935
    8. Data (including data from studies using RNA from post-mortem brain tissue) suggest that expression of DHCR24 is up-regulated in parietal cortex of patients with Huntington's disease as compared to control subjects. PMID: 24916565
    9. Data indicate a mechanism whereby 3beta-hydroxysterol Delta24-reductase (DHCR24) activity is regulated by signaling. PMID: 24363437
    10. DHCR24 expression protects neuronal cells from apoptotic cell death induced by endoplasmic reticulum stress. PMID: 24489783
    11. TR-beta and LXR-alpha competitively up-regulate the human Seladin-1 promoter, sharing the same response element, site A. PMID: 23416078
    12. DHCR24 gene expression is regulation by cholesterol availability. PMID: 23050906
    13. Inflammation is inhibited in coronary artery endothlial cells by increasing 3beta-hydroxysteroid-Delta24 reductase expression. PMID: 23123430
    14. a novel role for 24,25EC in cholesterol homeostasis, through its rapid inhibition of cholesterol synthesis at DHCR24. PMID: 22178193
    15. a gender dependent effect of DHCR24 rs600491 polymorphism on the susceptibility to Alzheimer disease. PMID: 22910610
    16. Activation of Sp1 by oxidative stress is involved in the promotion of expression of DHCR24 by Hepatitis C virus. PMID: 22431021
    17. This study describes Simvastatin modulates the Alzheimer's disease-related gene seladin-1. PMID: 21987590
    18. DHCR24-overexpressed cells were protected from apoptosis in response to oxidative stress, which was accompanied by a decrease in DHCR24 content on the ER and activation of caspase-3. PMID: 22010141
    19. affected individuals have a homozygous missense mutation in DHCR24 leading to dramatically augmented plasma desmosterol levels. We thus establish a clear consistent phenotype of desmosterolosis (MIM 602398). PMID: 21559050
    20. Level of brain amyloid deposition on [(11)C]PiB PET was associated with rs7551288, an intronic SNP in DHCR24, in 103 participants. The minor allele was associated with lower PiB uptake with non-carriers showing higher PiB uptake in frontal regions. PMID: 21901424
    21. DNA methylation and histone acetylation have roles in regulating the promoter region of the human DHCR24 gene PMID: 20568014
    22. Promoter methylation could be involved in the altered pattern of seladin-1 gene expression in adrenal carcinoma. PMID: 20465827
    23. Reduced expression of the enzyme that converts desmosterol into cholesterol, Alzheimer indicator 1 gene (seladin-1/dhcr24), in cortex and cerebellum may underlie increased desmosterol levels in 21 month-old amyloid-beta mutant mice. PMID: 20061631
    24. Seladin-1 involvement in proliferation and secretion suggests that its downregulation may be a major mechanism causing prostate cancer evolution. PMID: 20166102
    25. seladin-1/DHCR24 is modulated by the ACTH/cAMP-driven pathway and its expression is reduced in adrenal cancer PMID: 15001630
    26. unanticipated role for Seladin-1, previously implicated in Alzheimer's disease and cholesterol metabolism, in integrating cellular response to oncogenic and oxidative stress PMID: 15577914
    27. High levels of DHCR24 gene expression is associated with melanoma metastases PMID: 15688385
    28. seladin-1 has been isolated and found to be down-regulated in brain regions affected by Alzheimer disease--REVIEW PMID: 15954227
    29. These original results demonstrate for the first time that seladin-1 is abundantly expressed by stem cells and appear to suggest that reduced expression in AD might be due to an altered pool of multipotent cells. PMID: 16762343
    30. DHCR24 gene may be associated with Alzheimer's disease risk. PMID: 17510943
    31. Preliminary results suggest the absence of an association between DHCR24/seladin-1 genotypes and Alzheimer's disease in the Italian population. PMID: 17579359
    32. ablation of DHCR24/seladin-1 prevented apoptosis of primary neurons in a p53-dependent manner PMID: 17984220
    33. DHCR24 protects neuroblastoma cells against Abeta toxicity by increasing membrane cholesterol content. PMID: 18194465
    34. Seladin-1 may be considered a fundamental mediator of the neuroprotective effects of estrogen. PMID: 18499757
    35. the seladin-1 gene is androgen regulated and has a higher level of expression in prostate cancer tissues compared to the normal prostate PMID: 18762779
    36. Seladin-1/DHCR24 is an LXR target gene and that LXR may regulate lipid raft formation. PMID: 18815215
    37. Results suggest that antiinflammatory effects of HDLs are mediated partly through an upregulation of DHCR24. PMID: 19325144
    38. seladin-1 expression and intracellular localization are correlated with both the intensity and nature of ACTH-induced steroidogenesis and resultant oxidative stress. PMID: 19520779
    39. seladin-1 downregulation increases BACE1 levels and activity through enhanced GGA3 depletion during apoptosis PMID: 19815556
    40. DHCR24 is elevated in response to HCV infection and inhibits the p53 stress response by stimulating the accumulation of the MDM2-p53 complex in the cytoplasm and by inhibiting the acetylation of p53 in the nucleus. PMID: 19861417

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  • 相关疾病：
    Desmosterolosis (DESMOS)
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein.
  • 蛋白家族：
    FAD-binding oxidoreductase/transferase type 4 family
  • 组织特异性：
    Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. In the brain, strongly expressed in cortical regions, substan
  • 数据库链接：

    HGNC: 2859

    OMIM: 602398

    KEGG: hsa:1718

    STRING: 9606.ENSP00000360316

    UniGene: Hs.498727