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  4. Recombinant Human DNA replication licensing factor MCM7 (MCM7)

Recombinant Human DNA replication licensing factor MCM7 (MCM7)

  • 货号：
    CSB-YP013597HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013597HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013597HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013597HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013597HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CDABP0042; CDC 47; CDC47; CDC47 homolog; Cdc47; S. cerevisiae; homolog of; DNA replication licensing factor MCM7; Homolog of S. cerevisiae Cdc47; MCM 2; MCM 7; MCM2; MCM2; formerly; Mcm7; MCM7 minichromosome maintenance deficient 7; MCM7_HUMAN; Minichromosome Maintainence 7; Minichromosome maintainence; S. cerevisiae; homolog of; Minichromosome maintenance complex component 7; Minichromosome maintenance deficient 7; Minichromosome maintenance protein 7; P1.1 MCM3; P1.1-MCM3; P1CDC47; P85MCM; PNAS 146; PNAS146; PPP1R104; Protein phosphatase 1; regulatory subunit 104
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-719
  • 氨基酸序列
    ALKDYALEK EKVKKFLQEF YQDDELGKKQ FKYGNQLVRL AHREQVALYV DLDDVAEDDP ELVDSICENA RRYAKLFADA VQELLPQYKE REVVNKDVLD VYIEHRLMME QRSRDPGMVR SPQNQYPAEL MRRFELYFQG PSSNKPRVIR EVRADSVGKL VTVRGIVTRV SEVKPKMVVA TYTCDQCGAE TYQPIQSPTF MPLIMCPSQE CQTNRSGGRL YLQTRGSRFI KFQEMKMQEH SDQVPVGNIP RSITVLVEGE NTRIAQPGDH VSVTGIFLPI LRTGFRQVVQ GLLSETYLEA HRIVKMNKSE DDESGAGELT REELRQIAEE DFYEKLAASI APEIYGHEDV KKALLLLLVG GVDQSPRGMK IRGNINICLM GDPGVAKSQL LSYIDRLAPR SQYTTGRGSS GVGLTAAVLR DSVSGELTLE GGALVLADQG VCCIDEFDKM AEADRTAIHE VMEQQTISIA KAGILTTLNA RCSILAAANP AYGRYNPRRS LEQNIQLPAA LLSRFDLLWL IQDRPDRDND LRLAQHITYV HQHSRQPPSQ FEPLDMKLMR RYIAMCREKQ PMVPESLADY ITAAYVEMRR EAWASKDATY TSARTLLAIL RLSTALARLR MVDVVEKEDV NEAIRLMEMS KDSLLGDKGQ TARTQRPADV IFATVRELVS GGRSVRFSEA EQRCVSRGFT PAQFQAALDE YEELNVWQVN ASRTRITFV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
  • 基因功能参考文献：
    1. Our results indicate that MCM7 may exert certain functions on spindle formation to prevent cytokinesis during early mitosis by regulating CDK1 activity. PMID: 28588300
    2. MCM7 is helpful for us to make differential diagnosis in pathological grade, MCM7 combination of Ki67 may serve as more sensitive proliferation markers for evaluation of gastric carcinoma and precancerous lesions PMID: 28540486
    3. CDT1, MCM7, and NUDT1 were shown to be up-regulated in hepatocellular carcinoma tissues and provide a more accurate diagnosis than alpha-fetal protein alone. PMID: 29442275
    4. High expression of MCM-7 was reduced in BVP-treated cancer cells. PMID: 28628830
    5. Data indicate that RACK1 increases interactions between Akt and MCM7 and promotes Akt-dependent MCM7 phosphorylation, which in turn increases MCM7 binding to chromatin and miniature chromosome maintenance (MCM) complex formation. PMID: 28465488
    6. our findings suggested that MCM7 promoted tumor cell proliferation, colony formation and migration of ESCC cells via activating AKT1/mTOR signaling pathway PMID: 28498460
    7. Results proved that MCM7 proliferative index is a better method for identifying patients with risk of recurrence in patients surgically resected for meningiomas compared with the traditional methods used in the current clinical practice. PMID: 27868157
    8. MCM7-cyclin D1 pathway may participate in cancer progression. PMID: 28182015
    9. The MCM7 directly binds to the centrosomal linker protein Cep68 in vitro and complexes with Cep68 and VHL in vivo. PMID: 28578000
    10. The results clearly demonstrate 7q21-22 amplification, MCM7, and its intronic miR-25 are the major molecular switches involved in the complex oncogenic circuits of gastric cancer. PMID: 28059478
    11. High MCM7 expression is associated with non-small cell lung cancer. PMID: 27797825
    12. Results show that the expression of MCM7 gene and its microRNA, miR-106b-25 cluster is reduced under hypoxia. PMID: 28668890
    13. s found that MCM7 highly expressed in K562 cells rather than that in normal neutrophils. Thus, lentivirus-mediated shRNA targeting MCM7 was used to suppress its endogenous expression in K562 cells and develop a novel therapeutic strategy for leukemia. PMID: 28058629
    14. MCM7 polymorphisms were associated with survival rate and prognosis in acute myeloid leukemia patients. PMID: 27837251
    15. The BCL2, MCM7, and CCNE1 genes might play distinctive roles in cisplatin resistance in bladder cancer. PMID: 26966728
    16. High MCM-7 expression correlated with Cutaneous T-cell Lymphomas. PMID: 26504025
    17. KCTD5 is not involved in polyubiquitylation of MCM7 replication factor.Formation of trimeric complexes of KCTD5/cullin3 with MCM7, ZNF711 and FAM193B. PMID: 26188516
    18. MCM7 and G9a may serve as effective prognostic factors and could also be used as biomarkers for predicting various clinical outcomes of oesophageal squamous cell carcinoma PMID: 24805087
    19. MCM7 is essential for RNA splicing of epidermal growth factor receptor, c-Met, and platelet-derived growth factor receptor PMID: 25425645
    20. MCM7 expression may be a useful prognosis indicator in patients with diffuse-type gastric adenocarcinoma and its knockdown by RNA interference quenches its oncogenic property in gastric cancer PMID: 24647462
    21. We observed that lower levels of MCM7 correlate with reduced cell proliferation, lower autophagic capacity, and higher intracellular protein abundance phenotypes of aged and systemic sclerosis cells. PMID: 24496236
    22. Knockdown of MCM7 expression reduces glioblastoma multiforme cell proliferation and inhibits xenograft tumor growth in mice. PMID: 24166506
    23. expression of MCM7 is an independent risk factor for recurrence-free survival in patients with Dukes C colorectal cancer PMID: 25075101
    24. The A to G base change in SNP rs999885 of MCM7 may provide a protective effect on the prognosis of intermediate or advanced hepatocellular carcinoma in the Chinese. PMID: 24416400
    25. MCM 7, geminin and topo IIalpha can be reliable tools for the differential diagnosis of reactive mesothelial cells and malignant mesothelioma cells. PMID: 23860238
    26. MCM7 is a substrate of cyclin E/Cdk2 and can be phosphorylated on Ser-121. PMID: 23720738
    27. EGFR enhances MCM7 phosphorylation and DNA replication through Lyn phosphorylation in human cancer cells. PMID: 23764002
    28. MCM7 interacts with the receptor for activated protein kinase C 1 (RACK1), a protein kinase C (PKC) adaptor, in vivo and in vitro. PMID: 23313748
    29. Protein levels of MCM7 and p63 were associated significantly with high-grade cervical lesion, and aberrant p63 protein level may distinguish different histopathologic types of cervical carcinoma. PMID: 23318911
    30. the expression of cyclin D1, MCM7, TRIM29, and UBE2C was found to be significantly associated with progression to muscle-invasive bladder cancer. PMID: 23201130
    31. MCM7 expression may be a useful predictor of prognosis in patients with hepatocellular carcinoma after resection. PMID: 22784096
    32. MCM7 and Ki67 in tumor tissues may be potential markers of a poor prognosis for non-small cell lung cancer patients PMID: 22456526
    33. elevated expression of MCM7 to be associated with poor prognosis for patients with non-small cell lung cancer PMID: 21619671
    34. low level of MCM7 of proteins expression which may explain commonly known low mitotic activity of desmoid tumour cells PMID: 21478101
    35. MCM7 expression was significantly higher in high-grade serous carcinomas than in serous borderline tumors or other histological subtypes of ovarian cancer. PMID: 21076460
    36. Results demonstrate that interaction with Mcm7 is essential for the function of cyclin A in promoting S-phase entry. PMID: 21078875
    37. distribution pattern is similar to Ki-67;can be used as proliferation marker in experimental neoplasms PMID: 20048674
    38. Transcriptional activation of Mcm7 is mediated by HAT recruitment to the promoter region upon C. sinensis excretory-secretory products treatment. PMID: 20236609
    39. uncovered a proto-oncogenic miRNA-dependent network for PTEN regulation and defined the MCM7 locus as a critical factor in initiating prostate tumorigenesis PMID: 20388916
    40. MCM7 , critical for cell proliferation and maintenance of genome stability, are markedly down-regulated, Data hypothesized that their DNA-related functions could be partially limited in TRAIL-resistant HL-60 cells. PMID: 19834905
    41. Minichromosome maintenance protein MCM7 is a direct target of the MYCN transcription factor in neuroblastoma. PMID: 11861392
    42. Overexpressed MCM7 protein supports efficient DNA replication of Epstein-Barr virus oriP and rapid formation of tumors in nude mice without altering the activity of cellular DNA replication. PMID: 14550576
    43. MCM7 may be a highly informative biomarker for cervical cancer. PMID: 14678972
    44. hMCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells. PMID: 15538388
    45. interaction between endogenous Plk1 and Mcm7 was detected in a soluble chromatin fraction. These findings suggest a new function for Plk1 in coordination of DNA replication and mitotic events. PMID: 15654075
    46. MCM7 was found to be a more reliable and useful marker than Ki67 in assessing tumour proliferation and in the prognosis of patients. PMID: 15720416
    47. MCM7 amplification is responsible for markedly increased DNA synthesis, cell proliferation and an increased cell invasion in prostate cancer. PMID: 16247466
    48. p27Kip1 binds the conserved minichromosome maintenance domain of MCM7 proteins in a growth factor-dependent manner, such that the carboxyl terminal domain of p27Kip1 inhibits DNA replication independent of its function as a cyclin-dependent kinase PMID: 16289477
    49. MCM7, the c-myc-regulated gene is involved in genotype-C-HBV-related HCC, suggesting that c-myc is related to the hepatocarcinogenic activity of genotype-C HBV. PMID: 16703398
    50. Increase of the MCM7 is associated with tumor aggressiveness in astrocytoma PMID: 17094475

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    MCM family
  • 数据库链接：

    HGNC: 6950

    OMIM: 600592

    KEGG: hsa:4176

    STRING: 9606.ENSP00000307288

    UniGene: Hs.438720