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  4. Recombinant Human DNA replication complex GINS protein PSF1 (GINS1)

Recombinant Human DNA replication complex GINS protein PSF1 (GINS1)

  • 货号：
    CSB-YP617926HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP617926HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP617926HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP617926HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP617926HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GINS1
  • Uniprot No.：
  • 别名：
    DNA replication complex GINS protein PSF1; GINS complex subunit 1 (Psf1 homolog); GINS complex subunit 1; GINS1; partner of sld five-1; partner of sld5; PSF1 homolog; PSF1_HUMAN; RP4-691N24.2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-196
  • 氨基酸序列
    MFCEKAMELI RELHRAPEGQ LPAFNEDGLR QVLEEMKALY EQNQSDVNEA KSGGRSDLIP TIKFRHCSLL RNRRCTVAYL YDRLLRIRAL RWEYGSVLPN ALRFHMAAEE MEWFNNYKRS LATYMRSLGG DEGLDITQDM KPPKSLYIEV RCLKDYGEFE VDDGTSVLLK KNSQHFLPRW KCEQLIRQGV LEHILS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.
  • 基因功能参考文献：
    1. The s show that GINS is a compact tetramer in solution as observed in crystal structures, but also forms a double-tetrameric population, detectable by electron microscopy. PMID: 28071757
    2. Autosomal recessive, partial GINS1 deficiency impairs DNA replication and underlies intra-uterine (and postnatal) growth retardation, chronic neutropenia, and NK cell deficiency. PMID: 28414293
    3. PSF1 mRNA expression was an independent factor associated with prognosis of colorectal cancer. PMID: 21713706
    4. These results suggest that PSF1 over-expression is specifically involved in breast cancer cell growth. PMID: 20825491
    5. Findings implicate PSF1 in tumorigenesis and offer initial evidence of its potential as a theranostic target. PMID: 20103637
    6. expression of mammalian GINS is regulated by 17beta-Estradiol-stimulated estrogen receptor alpha, and PSF3 acts as a gene responsive to transcription factor E2F1 PMID: 17127213
    7. the C-terminal domain of Psf1 does not contribute to the stability of the GINS complex but is crucial for chromatin binding and replication activity PMID: 17417653

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    GINS1/PSF1 family
  • 数据库链接：

    HGNC: 28980

    OMIM: 610608

    KEGG: hsa:9837

    STRING: 9606.ENSP00000262460

    UniGene: Hs.658464