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  4. Recombinant Human DNA polymerase beta (POLB)

Recombinant Human DNA polymerase beta (POLB)

  • 货号：
    CSB-YP018302HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018302HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018302HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018302HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018302HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DNA directed DNA polymerase beta; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; DPOLB_HUMAN; MGC125976; Pol B; Pol beta; POLB; Polymerase (DNA directed) beta
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-335
  • 氨基酸序列
    MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.
  • 基因功能参考文献：
    1. point mutation (C-->G) in position 725 in exon 12, which shifts proline to arginine (P242R).This is a newly reported somatic mutation of pol beta in ovarian carcinoma patients from India PMID: 29524322
    2. Presently, we describe the preparation of three Pol beta enzymes modified at position 72 with aminooxy or hydrazinyl analogues of lysine. These enzymes form transient covalent bonds with the 5'-dRP moiety of the damaged DNA, in the form of an oxime or hydrazone, respectively PMID: 29625008
    3. MSH2-MSH3 not only stimulates pol beta to copy through the repeats but also enhances formation of the flap precursor for expansion. PMID: 27546332
    4. Bond formation and cleavage reactions catalyzed by base excision repair DNA polymerases beta and lambda has been described. PMID: 27992186
    5. POLB, is located in the mitochondria and plays a significant role in mitochondrial BER, mtDNA integrity and mitochondrial function. [REVIEW] PMID: 29129598
    6. In the mitochondrial extracts from APTX-/- cells, the strong pol beta lyase and FEN1 activities appear able to repair the blocked BER intermediates with 5'-AMP-dRP groups.Neither pol gamma lyase nor FEN1 alone is able to complement APTX deficiency in the absence of pol beta. PMID: 28973450
    7. human polymerase beta, accommodates 8-oxoG at the primer terminus opposite cytosine and adenine. PMID: 28449123
    8. POLB functions in base excision repair to fill in single-nucleotide gaps and exhibits mutation E288K associated with colon cancer. Data suggest that E288K mutant POLB exhibits faster rate of closing of fingers domain combined with slower rate of nucleotide release compared to wild-type POLB; loss of fidelity appears due to defect in interaction of E288K mutant POLB with DNA, resulting in stable, closed enzyme structure. PMID: 28945359
    9. Unencumbered polymerase beta lyase activity in nucleosome core particles during base excision repair has been reported. PMID: 28911106
    10. pol beta and BRCA1 appeared to have a functional interaction, observed in both DT40 and human cell lines, in protecting cells against alkylating MMS-induced cytotoxicity; but pol beta had no protective effect in the absence of BRCA1. In vivo and in vitro assays for a base excision repair role of BRCA1 were negative. PMID: 23826138
    11. hPolbeta discriminates against L-stereochemistry through accumulation of several active site rearrangements that lead to a decreased nucleotide binding affinity and incorporation rate. The two NRTIs escape some of the active site selection through the base and sugar modifications but are selected against through the inability of hPolbeta to complete thumb domain closure. PMID: 28402499
    12. pol beta contains a specific NLS sequence in the N-terminal lyase domain that promotes transport of the protein independent of its interaction partners. Active nuclear uptake allows development of a nuclear/cytosolic concentration gradient against a background of passive diffusion. PMID: 27956495
    13. Data suggest that POLB activity is not essential for HIV-1 integration into human macrophages and other cells; limited cellular dNTP pools, but not POLB expression, are primary factor for HIV-1 DNA gap repair, particularly in non-dividing cells. PMID: 28684413
    14. High DNA polymerase beta mRNA expression is associated with Graves' thyroid tissue. PMID: 27848228
    15. Polbeta was shown to inhibit the 3'->5' exonuclease activity of APE1 when both enzymes were added simultaneously and to insert the correct nucleotide into the gap arising after hydrolysis of AP site clustered with the benzo[a]pyrene adducts. PMID: 28065385
    16. Remote mutations induce functional changes in active site residues of human DNA polymerase beta. PMID: 28402631
    17. It has been concluded based on calculations and the experimental pH profile that the most likely path for the wild-type (WT) and the D256E and D256A mutants is a proton transfer (PT) to the bulk, although the WT may also use a PT to Asp 256. PMID: 27488241
    18. The homozygous/heterozygous genotypes containing the African allele showed higher POLB expression than the homozygous white allele genotype (P < .001). A replication study using a GEO data set validated all 5 eQTLs and also showed a statistically significant difference in POLB expression based on genetic ancestry (P = .002). PMID: 27906459
    19. HMGB1 stimulates activity of DNA polymerase beta on nucleosome substrates in the process of base excision repair. PMID: 28098985
    20. The results help to define the tolerance of DNA Pol beta to subtle local structural and functional alterations derived from lysine 72. PMID: 28005340
    21. PolB is ubiquitylated on amino-acid residues K206 and K244 when not in a complex with XRCC1. PMID: 25423885
    22. a substitution mutation, R152C, impairs Pol beta polymerase activity and DNA base excision repair efficiency. PMID: 26760506
    23. These results suggest that polbeta gene with G648C mutation in surgically resected esophagus may be clinically useful for predicting responsiveness to chemotherapy in patients with esophageal cancer PMID: 26334617
    24. Data show that esophageal carcinoma (EC) cells expressing the DNA polymerase beta K167I variant to be sensitive to DNA damaging agents. PMID: 26527528
    25. Pol lambda can incorporate rNMPs opposite normal bases or 8-oxo-G, and with a different fidelity. Further, the incorporation of rNMPs opposite 8-oxo-G delays repair by DNA glycosylases. PMID: 26917111
    26. POLB was pinpointed as a target gene of miR- 499. Additionally, we identified that miR-499 can enhance cisplatin's function of inhibiting proliferation and of promoting apoptosis in EC9706 and KYSE30 cell lines. PMID: 26159783
    27. Since APE1, XRCC1 and POLB proteins operate downstream the same DNA repair pathway to eliminate DNA oxidative damage, their expression above basal levels found in our study could reveal excessive oxidative stress in high-grade bladder tumors. PMID: 26238022
    28. findings indicate that the IKBKB and POLB SNPs confer no genetic predisposition to SLE risk in this Chinese Han population PMID: 26167925
    29. The structure of pol beta in binary complex with template 8-oxoG in a base excision repair substrate, is reported.[Dna polymerase beta] PMID: 22178760
    30. Fen1 significantly stimulated trinucleotide repeats expansion by Pol beta, but not by the related enzyme Pol lambda. PMID: 25687118
    31. Data show that tongue cancer resistance-associated protein 1 (TCRP1) contributes to cisplatin (DDP) resistance via the increased expression of DNA polymerase beta subunit (Pol beta) in lung cancer cells. PMID: 25260657
    32. DNA polymerase beta catalyzes lesion bypass across benzo[a]pyrene-derived DNA adduct in an error prone manner. PMID: 25460917
    33. HSP90 regulates DNA repair by interaction between XRCC1 and DNA polymerase beta. PMID: 25423885
    34. The results suggest that POLB gene with T889C mutation in surgically resected primary gastric tissues may be clinically useful for predicting responsiveness to chemotherapy in patients with GC. PMID: 25561897
    35. A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells. PMID: 25879709
    36. The study provides new insights into the spontaneous replication error and the replication fidelity mechanisms of DNA polymerase beta. PMID: 25200079
    37. structural analysis of the mechanism of polbeta-mediated cisplatin resistance PMID: 25237188
    38. analysis of DNA polymerase beta activation PMID: 25261471
    39. Upregulation of DNA polymerase beta is associated with chemoresistance in esophageal carcinoma. PMID: 24460306
    40. that polbeta silencing increased the sensitivity of esophageal carcinoma cells to radiation therapy PMID: 25015190
    41. POLB deficiency is associated with aggressive breast cancer and poor prognosis. PMID: 24462520
    42. Pols beta from human cells to act on DNA containing an isolated 2-deoxyribonolactone, as well as when the lesion comprises the 5'-component of a tandem lesion. PMID: 23101935
    43. S229L colon tumor-associated variant of DNA polymerase beta induces cellular transformation as a result of decreased polymerization efficiency PMID: 24668809
    44. variant metal-ion routes in DNA polymerase beta's conformational pathways PMID: 24511902
    45. This study reveals that DNA polymerase beta catalysis across N7-methylguanine is slow, yet highly accurate. PMID: 24966350
    46. shown that germline and tumor-associated variants of DNA polymerase beta (Pol ss) catalyze aberrant base excision repair (BER) that leads to genomic instability and cellular transformation. PMID: 24348210
    47. results identified nine types of mutations within polbeta cDNA in esophageal squamous cell carcinoma patients with four mutations related to patient survival PMID: 23959474
    48. We conclude that distinct mutations of the triple pol b mutant are responsible for the loss of activity, lower fidelity, and instability observed in vitro. PMID: 23877444
    49. Comparison with published structures provides insight into the structural rearrangements within pol b that occur during the process of nucleotide discrimination. PMID: 24133209
    50. Upregulation of transcriptional activity induced by mutations in the DNA polbeta promoter may be one of the molecular mechanisms mediating abnormal overexpression of DNA polbeta in esophageal squamous cell carcinoma. PMID: 23749489

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.
  • 蛋白家族：
    DNA polymerase type-X family
  • 数据库链接：

    HGNC: 9174

    OMIM: 174760

    KEGG: hsa:5423

    STRING: 9606.ENSP00000265421

    UniGene: Hs.654484