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  4. Recombinant Human DNA fragmentation factor subunit beta (DFFB)

Recombinant Human DNA fragmentation factor subunit beta (DFFB)

  • 货号：
    CSB-YP006738HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006738HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006738HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006738HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CAD; Caspase activated deoxyribonuclease ; Caspase activated DNase; Caspase activated nuclease; Caspase-activated deoxyribonuclease; Caspase-activated DNase; Caspase-activated nuclease; CPAN; Deoxyribonuclease III, caspase activated; DFF 40; DFF-40; DFF2; DFF40; Dffb; DFFB_HUMAN; Didff; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase); DNA fragmentation factor, 40 kD beta subunit; DNA fragmentation factor, 40 kD, beta polypeptide; DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase); OTTHUMP00000003633
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-338
  • 氨基酸序列
    MLQKPKSVKL RALRSPRKFG VAGRSCQEVL RKGCLRFQLP ERGSRLCLYE DGTELTEDYF PSVPDNAELV LLTLGQAWQG YVSDIRRFLS AFHEPQVGLI QAAQQLLCDE QAPQRQRLLA DLLHNVSQNI AAETRAEDPP WFEGLESRFQ SKSGYLRYSC ESRIRSYLRE VSSYPSTVGA EAQEEFLRVL GSMCQRLRSM QYNGSYFDRG AKGGSRLCTP EGWFSCQGPF DMDSCLSRHS INPYSNRESR ILFSTWNLDH IIEKKRTIIP TLVEAIKEQD GREVDWEYFY GLLFTSENLK LVHIVCHKKT THKLNCDPSR IYKPQTRLKR KQPVRKRQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
  • 基因功能参考文献：
    1. we show that executioner caspase activation of the apoptotic nuclease CAD/DFF40 is essential for TRAIL-induced mutations in surviving cells. As exposure to chemotherapy drugs also activates apoptotic caspases and presumably CAD, we hypothesized that these pathways may also contribute to the mutagenesis induced by conventional chemotherapy drugs, perhaps augmenting the mutations that arise from direct DNA damage PMID: 28981092
    2. Dff40 expression is upregulated in atherosclerotic plaque. PMID: 28007744
    3. the low expression levels of DFF40/CAD and the absence of DNA laddering as common molecular traits in glioblastoma PMID: 26755073
    4. Data suggest DFF40 expression in breast cancer cell line is involved in drug sensitivity/resistance to doxorubicin; apoptotic cell death due to doxorubicin (a topoisomerase II inhibitor) is enhanced by DFF40 overexpression in breast cancer cell line. PMID: 26529233
    5. Combinatorial use of some sulfonamides such as acetazolamide along with increased expression of DFF40 can potently kill tumor cells via apoptosis. PMID: 25086620
    6. DFF40/CAD-independent mechanism driving conformational nuclear changes during caspase-dependent cell death PMID: 24838313
    7. the highest order of chromatin compaction observed in the later steps of caspase-dependent apoptosis relies on DFF40/CAD-mediated DNA damage by generating 3'-OH ends in single-strand rather than double-strand DNA nicks/breaks PMID: 23430749
    8. These results suggest a cooperative activity between CAD and DNAS1L3 to accomplish internucleosomal DNA fragmentation . PMID: 23229555
    9. Human papillomavirus type 16 E6 protein inhibits DNA fragmentation via interaction with DNA fragmentation factor 40 PMID: 22609799
    10. During apoptotic rearrangement of interchromatin granule clusters, the nuclear matrix (NuMa rearrangement) and chromatin are closely associated. This process occurs in defined stages and depends on the activity of protein phosphatases, caspases and CAD. PMID: 22023725
    11. the cytosolic levels of DFF40/CAD are determinants in achieving a complete apoptotic phenotype, including oligonucleosomal DNA degradation. PMID: 22253444
    12. Data show that among the 13 SNPs in the 3 genes, only 3 were found to be polymorphic: R196K and K277R in the DFFB gene, and S12L in the EndoG gene, and all 6 SNPs in the FEN-1 gene were entirely monoallelic. PMID: 22011247
    13. These data suggest that DFF 40 mediated apoptosis plays a significant role in mediating sepsis induced cellular dysfunction. PMID: 21820410
    14. Mutations and aberrantly spliced transcripts for the CAD gene are frequently associated with hepatocellular carcinoma. PMID: 12610505
    15. Hsp70 binds free CAD in TCR-stimulated T cells to stabilize and augment its activity. PMID: 12738667
    16. CAD involves unrestricted accessibility of chromosomal DNA at the initial phase of apoptosis, followed by its nuclear immobilization that may prevent the release of the active nuclease into the extracellular environment. PMID: 15569712
    17. PARP-1 poly(ADP-ribosyl)ation is a terminal event in the apoptotic response that occurs in response to DNA fragmentation and directly influences DFF40 activity PMID: 15703174
    18. Interactions identified here between human placenta histone H1 carboxyl-terminal domain and DFF40/CAD target and activate linker DNA cleavage during the terminal stages of apoptosis. PMID: 15910001
    19. Our findings of high frequency of Alu-mediated hCAD deletion in human hepatoma underscore the implication of hCAD in hepatocarcinogenesis PMID: 16007181
    20. AIF is responsible for stage I nuclear morphology and HMW DNA degradation is a caspase-activated DNase and AIF-independent process PMID: 16049016
    21. levels of CAD were significantly higher in the nuclear fraction of temporal lobe epilepsy samples PMID: 16121124
    22. DFFB haploinsufficiency from 1p allelic loss is a contributing factor in oligodendroglioma development PMID: 16156899
    23. in apoptotic cells, endogenous and exogenous CAD forms limited oligomers, representing the active nuclease PMID: 16204257
    24. the N-terminal region was found to be responsible for the requirement of salt for fibril formation PMID: 16428311
    25. CAD is downregulated at the mRNA and protein level during the erythroid differentiation in TF-1 cells. PMID: 16529748
    26. poly-glutamic acid and heparin inhibit DFF40/CAD, the latter one being highly effective at nanomolar concentrations. The inhibitory poly-anions bind to the nuclease and impair its ability to bind double-stranded DNA. PMID: 16699957
    27. These results suggest that CAD is the endogenous endonuclease that mediates internucleosomal DNA degradation in rotenone-induced apoptosis. PMID: 17239993
    28. Data suggest that erythroblast chromatin degradation may involve caspase activated DNase and apoptosis inducing factor, enzymes distinct from those active in apoptotic cells. PMID: 17492772
    29. changes induced in DNA conformation upon HMG-box binding makes the substrate more accessible to cleavage by DFF40/CAD nuclease and thus may contribute to preferential linker DNA cleavage during apoptosis PMID: 18239742
    30. We have found that neither single-stranded DNA, single-stranded RNA, double-stranded RNA nor RNA-DNA heteroduplexes are cleaved by the DFF40/CAD nuclease. PMID: 18283539

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 数据库链接：

    HGNC: 2773

    OMIM: 601883

    KEGG: hsa:1677

    STRING: 9606.ENSP00000367454

    UniGene: Hs.133089