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  4. Recombinant Human DNA (cytosine-5)-methyltransferase 3-like (DNMT3L)

Recombinant Human DNA (cytosine-5)-methyltransferase 3-like (DNMT3L)

  • 中文名称：
    人DNMT3L重组蛋白
  • 货号：
    CSB-YP891717HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人DNMT3L重组蛋白
  • 货号：
    CSB-EP891717HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人DNMT3L重组蛋白
  • 货号：
    CSB-EP891717HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人DNMT3L重组蛋白
  • 货号：
    CSB-BP891717HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人DNMT3L重组蛋白
  • 货号：
    CSB-MP891717HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DNMT3L
  • Uniprot No.：
  • 别名：
    Cytosine 5 methyltransferase 3 like protein; DNA (cytosine 5 ) methyltransferase 3 like; DNA (cytosine-5)-methyltransferase 3-like; DNA cytosine 5 methyltransferase 3 like protein; DNA methyltransferase 3 like protein; DNM3L_HUMAN; Dnmt 3L; Dnmt3l; Human cytosine 5 methyltransferase 3 like protein; MGC1090
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-386
  • 氨基酸序列
    MAAIPALDPE AEPSMDVILV GSSELSSSVS PGTGRDLIAY EVKANQRNIE DICICCGSLQ VHTQHPLFEG GICAPCKDKF LDALFLYDDD GYQSYCSICC SGETLLICGN PDCTRCYCFE CVDSLVGPGT SGKVHAMSNW VCYLCLPSSR SGLLQRRRKW RSQLKAFYDR ESENPLEMFE TVPVWRRQPV RVLSLFEDIK KELTSLGFLE SGSDPGQLKH VVDVTDTVRK DVEEWGPFDL VYGATPPLGH TCDRPPSWYL FQFHRLLQYA RPKPGSPRPF FWMFVDNLVL NKEDLDVASR FLEMEPVTIP DVHGGSLQNA VRVWSNIPAI RSRHWALVSE EELSLLAQNK QSSKLAAKWP TKLVKNCFLP LREYFKYFST ELTSSL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context. Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain. Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. Plays a key role in embryonic stem cells and germ cells. In germ cells, required for the methylation of imprinted loci together with DNMT3A. In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization. Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation. While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes. Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes. Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression.
  • 基因功能参考文献：
    1. DNMT3L overexpression is associated with Down syndrome. PMID: 26911678
    2. the present study has demonstrated that variations in the DNMT3L gene do not contribute to stage I-II endometriosis-associated infertility. PMID: 26647998
    3. DNMT3L rs2070565 (genotype P = 0.007, allele P = 0.0026) confers an increased risk of developing schizophrenia at an early age in individuals with family history. PMID: 24859147
    4. crystal structures of DNMT3A-DNMT3L (autoinhibitory form) and DNMT3A-DNMT3L-H3 (active form) complexes at 3.82 and 2.90 A resolution, respectively PMID: 25383530
    5. DNMT3L can address DNMT3A/B to specific sites by directly interacting with TFs that do not directly interact with DNMT3A/B PMID: 24952347
    6. CpG island encompassing the promoter and first exon of human DNMT3L gene is a PcG/TrX response element PMID: 24743422
    7. DNMT3L is one of the key players in de novo DNA methylation of imprinting control elements and retrotransposons, which occurs after genome-wide epigenetic erasure during germ cell development. (Review) PMID: 22671959
    8. SNP rs2070565, as well as haplotypes AAA and GAA, may be associated with male infertility; DNMT3L may contribute to azoospermia susceptibility in humans PMID: 22116073
    9. Genetic polymorphisms of DNMT3L involved in hypermethylation of chromosomal ends are associated with greater risk of developing ovarian endometriosis. PMID: 22401780
    10. mutation analysis of SYCP3, DNMT3L and MSH4 in patients with maturation arrest of spermatogenesis and couples with recurrent miscarriages. PMID: 21126912
    11. This study offers insights into the manner by which DNA methylation patterns are deposited and reveals a new level of interplay between members of the de novo DNMT family. PMID: 20838592
    12. Processive methylation is enhanced 3-fold in the presence of DNMT3L, an inactive homolog of DNMT3A, therefore providing a mechanism for the previously described DNMT3L activation of DNMT3A. PMID: 20630873
    13. We have identified loss of methylation at the DNMT3L promoter in ocular surface squamous neoplasia cases. PMID: 20670142
    14. DNMT3L is a novel marker and is essential for the growth of human embryonal carcinoma. PMID: 20460473
    15. DNMT3L exerts a major effect on the transcriptional regulation of a specific target gene, such as thymine DNA glycosylase PMID: 20428781
    16. CG dinucleotide recognized by the Dnmt3a and Dnmt3L complex are distinctive at retroelements and imprinted domains. PMID: 19921333
    17. DNMT3L stimulates de novo methylation by Dnmt3a. PMID: 12481029
    18. The carboxyl-terminal half of DNMT3L was found to be responsible for the stimulation of the DNA methylation activity of Dnmt3a and Dnmt3b. PMID: 15105426
    19. Regulates germ cell-specific gene expression and intracisternal A-particle suppression, which are critical for male germ cell proliferation and meiosis. PMID: 16211598
    20. Dnmt3L-Dnmt3b interactions play an important role in the regulation of DNA methylation during mammalian development. PMID: 16543361
    21. The acquisition of DNMT3L by a common ancestor of eutherians and marsupials might have been closely related to the evolution of imprinting. PMID: 16575165
    22. Data show that binding of DNMT3L to DNMT3A2 promotes reorganization of DNMT3A2 subunits and leads to formation of specific complexes with enhanced DNA methyltransferase activity and increased S-adenosyl-L-methionine binding. PMID: 16829525
    23. Crystallographic studies of human DNMT3L showed that the protein has a carboxy-terminal methyltransferase-like domain and an N-terminal cysteine-rich domain PMID: 17687327
    24. the carboxy-terminal domain of human Dnmt3L interacts with the catalytic domain of Dnmt3a, demonstrating that Dnmt3L has dual functions of binding the unmethylated histone tail and activating DNA methyltransferase PMID: 17713477
    25. Importance of DNA methylation profile at DNMT3L promoter not only as a promising biomarker for cervical cancer but also provides insight into the possible role of DNMT3L in cancer development. PMID: 17965599
    26. DNMT3L(R271Q) is impaired in its ability to stimulate de novo DNA methylation by DNMT3A. PMID: 19246518
    27. Overexpression of DNMT3L, which functions by regulating the activity of DNMT3A and DNMT3B, increased cellular proliferation and anchorage-independent growth. PMID: 19625766

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed at low levels in several tissues including testis, ovary, and thymus.
  • 数据库链接：

    HGNC: 2980

    OMIM: 606588

    KEGG: hsa:29947

    STRING: 9606.ENSP00000270172

    UniGene: Hs.592165