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  4. Recombinant Human Cytochrome P450 2W1 (CYP2W1)

Recombinant Human Cytochrome P450 2W1 (CYP2W1)

  • 货号：
    CSB-YP823444HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP823444HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP823444HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP823444HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP823444HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CYP2W1
  • Uniprot No.：
  • 别名：
    CP2W1_HUMAN; CYP2W 1; CYP2W1; CYPIIW1; Cytochrome P450 2W1; Cytochrome P450; family 2; subfamily W; polypeptide 1; MGC34287
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-490
  • 氨基酸序列
    MALLLLLFLG LLGLWGLLCA CAQDPSPAAR WPPGPRPLPL VGNLHLLRLS QQDRSLMELS ERYGPVFTVH LGRQKTVVLT GFEAVKEALA GPGQELADRP PIAIFQLIQR GGGIFFSSGA RWRAARQFTV RALHSLGVGR EPVADKILQE LKCLSGQLDG YRGRPFPLAL LGWAPSNITF ALLFGRRFDY RDPVFVSLLG LIDEVMVLLG SPGLQLFNVY PWLGALLQLH RPVLRKIEEV RAILRTLLEA RRPHVCPGDP VCSYVDALIQ QGQGDDPEGL FAEANAVACT LDMVMAGTET TSATLQWAAL LMGRHPDVQG RVQEELDRVL GPGRTPRLED QQALPYTSAV LHEVQRFITL LPHVPRCTAA DTQLGGFLLP KGTPVIPLLT SVLLDETQWQ TPGQFNPGHF LDANGHFVKR EAFLPFSAGR RVCVGERLAR TELFLLFAGL LQRYRLLPPP GVSPASLDTT PARAFTMRPR AQALCAVPRP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase that may play a role in retinoid and phospholipid metabolism. Catalyzes the hydroxylation of saturated carbon hydrogen bonds. Hydroxylates all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may regulate atRA clearance. Other retinoids such as all-trans retinol and all-trans retinal are potential endogenous substrates. Catalyzes both epoxidation of double bonds and hydroxylation of carbon hydrogen bonds of the fatty acyl chain of 1-acylphospholipids/2-lysophospholipids. Can metabolize various lysophospholipids classes including lysophosphatidylcholines (LPCs), lysophosphatidylinositols (LPIs), lysophosphatidylserines (LPSs), lysophosphatidylglycerols (LPGs), lysophosphatidylethanolamines (LPEs) and lysophosphatidic acids (LPAs). Has low or no activity toward 2-acylphospholipids/1-lysophospholipids, diacylphospholipids and free fatty acids. May play a role in tumorigenesis by activating procarcinogens such as aflatoxin B1, polycyclic aromatic hydrocarbon dihydrodiols and aromatic amines. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
  • 基因功能参考文献：
    1. showed that the genetic pattern of CYP2W1 is interethnically different among the three Chinese populations, and this finding can extend our understanding of population genetics of CYP2W1 in the Chinese population PMID: 27307299
    2. summarizes the history, distribution, polymorphism, membrane topology, function and possible role in cancer therapy of CYP2W1. PMID: 27257736
    3. Normal adrenal tissue lacks CYP2W1 enzyme expression; adrenocortical carcinomas generally do not express the enzyme as well. PMID: 27598485
    4. Report novel SNPs of CYP2W1 gene in Chinese Uygur and Han populations. PMID: 26683388
    5. Data suggest that canonical cytochrome P-450 reductants (cytochrome P450 reductase; cytochrome b5) cannot serve as electron donors for CYP2W1, a colon tumor-specific enzyme; glycosylation of CYP2W1 at Asn177 is required for full catalytic activity. PMID: 26787547
    6. Results show the developmental expression of CYP2W1 in the GI tract mediated by epigenetic modifications, its specific location, and regulation by anticancer drugs which can be considered as an adjuvant therapy of colon and hepatic cancers. PMID: 25844926
    7. Low KRT13 mRNA expression is associated with oral squamous cell carcinoma. PMID: 25735388
    8. CYP2W1 is highly expressed in both normal and neoplastic adrenal glands making it a promising tool for targeted therapy in ACC PMID: 25144458
    9. High CYP2W1 expression is seen in 26% of primary colorectal tumors and in 48% of corresponding liver metastases. PMID: 24625228
    10. CYP2W1 could play an important role in hepatocellular carcinoma (HCC) and might serve as a valuable prognostic marker and potential target for gene therapy in the treatment of HCC. PMID: 24801906
    11. No significant differences in the distribution of CYP2W1*1, CYP2W1*2 and CYP2W1*6 alleles were found between CRC patients and controls. PMID: 24088132
    12. Results of the current study were in agreement with those of the previous pilot study and show that higher expression of CYP2W1 seems to be of prognostic value in colon cancer. PMID: 22993331
    13. study did not detect an association between the CYP2W1, 4F11 and 8A1 genes polymorphisms and breast cancer risk in a Mexican population; however, some clinico-pathological risk factors interact with CYP2W1 genotypes and modifies susceptibility to breast cancer PMID: 22631658
    14. a role for human cytochrome P450 2W1 in selective oxidation of lysophospholipids PMID: 22591743
    15. CYP2W1 variant alleles are common among Caucasian individuals and, of these, the CYP2W1 G541A (Ala181Thr) polymorphism is associated with increased colorectal cancer risk. PMID: 20602611
    16. CYP2W1 is catalytically active in the transformation of aflatoxin B1 to cytotoxic products and in the metabolism of indolines, indicating functional intracellular electron transfer. PMID: 20805301
    17. CYP2W1 is expressed human colonic and adrenal gland neoplasms, but not normal tissue, and is expressed in rat fetal and adult colon PMID: 16426568
    18. The expression of CYP2W1 is colon tumor-specific and is associated with methylation status of the CYP2W1 gene, suggesting a potential causal link between the gene hypomethylation and its enhanced expression. PMID: 17979506
    19. Six single nucleotide polymorphisms of CYP2W1 were identified in a Japanese population. PMID: 17998294

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  • 亚细胞定位：
    Endoplasmic reticulum lumen. Cell membrane. Microsome membrane.
  • 蛋白家族：
    Cytochrome P450 family
  • 组织特异性：
    Very low levels are detected in fetal and adult tissues. Highly expressed in several tumor samples, in particular colon and adrenal tumors.
  • 数据库链接：

    HGNC: 20243

    OMIM: 615967

    KEGG: hsa:54905

    STRING: 9606.ENSP00000310149

    UniGene: Hs.272795