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  4. Recombinant Human Cytochrome P450 1A1 (CYP1A1)

Recombinant Human Cytochrome P450 1A1 (CYP1A1)

  • 货号：
    CSB-EP006395HUb0
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    CYP1A1
  • Uniprot No.：
  • 别名：
    AHH; AHRR; Aryl hydrocarbon hydroxylase; CP11; CP1A1_HUMAN; CYP 1; CYP1; cyp1a1; CYPIA1; Cytochrome P1 450 dioxin inducible; Cytochrome P1-450; Cytochrome P450 1A1; Cytochrome P450 family 1 subfamily A polypeptide 1; Cytochrome P450 form 6; Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1; Cytochrome P450-C; Cytochrome P450-P1; Flavoprotein-linked monooxygenase; Microsomal monooxygenase; P1 450; P450 C; P450 form 6; P450 P1; P450DX; Xenobiotic monooxygenase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    61.8 kDa
  • 表达区域：
    1-512aa
  • 氨基酸序列
    MLFPISMSATEFLLASVIFCLVFWVIRASRPQVPKGLKNPPGPWGWPLIGHMLTLGKNPHLALSRMSQQYGDVLQIRIGSTPVVVLSGLDTIRQALVRQGDDFKGRPDLYTFTLISNGQSMSFSPDSGPVWAARRRLAQNGLKSFSIASDPASSTSCYLEEHVSKEAEVLISTLQELMAGPGHFNPYRYVVVSVTNVICAICFGRRYDHNHQELLSLVNLNNNFGEVVGSGNPADFIPILRYLPNPSLNAFKDLNEKFYSFMQKMVKEHYKTFEKGHIRDITDSLIEHCQEKQLDENANVQLSDEKIINIVLDLFGAGFDTVTTAISWSLMYLVMNPRVQRKIQEELDTVIGRSRRPRLSDRSHLPYMEAFILETFRHSSFVPFTIPHSTTRDTSLKGFYIPKGRCVFVNQWQINHDQKLWVNPSEFLPERFLTPDGAIDKVLSEKVIIFGMGKRKCIGETIARWEVFLFLAILLQRVEFSVPLGVKVDMTPIYGLTMKHACCEHFQMQLRS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions. Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system. Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer. May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent).
  • 基因功能参考文献：
    1. Results indicate that the underlying mechanisms whereby etoposide and ellipticine regulate CYP1A1 expression must be different and may not be linked to p53 activation alone. PMID: 29471073
    2. rs2470893 SNP, which maps 196 bp from a CYP1A1 promoter XRE, is associated with variations in 3MC-dependent AHR binding and CYP1A1 expression. PMID: 29980579
    3. study demonstrates the importance of CYP1A1 gene expression as well as organochlorine pesticides levels in the context of urinary bladder cancer risk. PMID: 29027484
    4. The study revealed association of CYP1A1 I462V with Infectious Endocarditis. PMID: 30289676
    5. Differential methylation was observed in the CYP1A1 promoter and AHRR gene body regions between women who smoked throughout pregnancy and non-smokers on the fetal-side of the placenta and in the GFI1 promoter between women who quit smoking while pregnant and non-smokers on the maternal-side of the placenta PMID: 28820654
    6. Our meta-analysis supports that CYP1A1 exon7 polymorphism might contribute to individual susceptibility to esophageal cancer in the Chinese population. Gene-gene interaction analysis found a synergistic interaction between CYP1A1 mutation genotypes and GSTM1 deletion genotype. PMID: 28164573
    7. For CYP1A1, exposure to maternal smoking was not associated with fetal DNA methylation changes whereas mRNA expression increased in placentas and male fetal livers. PMID: 29169084
    8. SNP variants of CYP1A1, rs1048943 (A4889G), rs4646903 (T3801C), were typed in Mexican women with breast cancer and normal controls. There was no significant association. PMID: 29267651
    9. Study shows the effect of dietary flavonoid intake on colorectal cancer risk differs according to flavonoid subclasses and CYP1A1 genetic variants. PMID: 28273931
    10. CYP1A1 mRNA expression in the preputial tissues from patients with hypospadias and phimosis PMID: 29080015
    11. The association between persistent exposure to PM10 and lung function decline in Korean men may be determined in part by several functional variants of the CYP1A1 gene. PMID: 29627601
    12. These observations reveal a regulatory role of CYP1A1 in liver lipid peroxidation and imply CYP1A1 as a potential therapeutic target. PMID: 29738693
    13. genetic association studies in population of postmenopausal Mexican-Mestizo women: Data suggest SNPs in CYP1A1 (rs1799814, rs1048943; but not rs1056836 in CYP1B1) are associated with postmenopausal osteoporosis in the population studied; SNPs in CYP1A1 are associated with low bone mineral density in lumbar spine and femur neck. (CYP1A1 = aryl hydrocarbon hydroxylase CYP1A1; CYP1B1 = aryl hydrocarbon hydroxylase CYP1B1) PMID: 28300467
    14. Meta-analysis showed that there was an interaction between the CYP1A1 MspI and smoking on the risk of lung cancer in the Chinese population. [Meta-Analysis] PMID: 26918783
    15. The SNPs in CYP1A1, GSTP1 and XRCC1 genes did not show significant association with complete remission (CR) rate, overall survival (OS) or event free survival (EFS). However, XRCC1 Arg194Trp SNP was associated with higher drug toxicity; carriers of variant genotypes (CT and TT) had a significantly higher frequency of myelosuppression compared to those with the wild CC genotype PMID: 28844589
    16. Our findings revealed that I3C inhibits the proliferation of THP-1 cells in a dose- and time-dependent manner with minimal toxicity over normal monocytes. The AhR target genes (CYP1A1, IL1beta) were overexpressed upon I3C treatment PMID: 28812970
    17. Data suggest that response of bronchial epithelial cells to environmental carcinogen benzo[a]pyrene includes activation of AhR/Src/ERK signaling, CYP1A1 induction, and formation of stable DNA adducts. (AhR = aryl hydrocarbon receptor; Src = Src proto-oncogene kinase; ERK = extracellular signal-regulated kinases; CYP1A1 = cytochrome P450 family 1 subfamily A member 1) PMID: 29545172
    18. This meta-analysis demonstrated that polymorphisms in MspI and Ile462Val of CYP1A1 were risk factors for developing cervical cancer. PMID: 29595663
    19. Computational molecular modeling tools have been used by several research groups to decipher the specificity and potency factors of the CYP1A1 and CYP1A2 substrates. In this review, we perform a thorough analysis of the computational studies that are ligand-based and protein-ligand complex-based to catalog the various factors that govern the specificity/potency toward these two enzymes PMID: 28698457
    20. Results showed that polymorphism in PPARGrs10865710, PPARGrs1805192 and CYP1A1rs4646903 and interaction between PPARGrs1805192 and CYP1A1rs4646903 were related with increased coronary artery disease susceptibility. PMID: 28415751
    21. The increased risk of prostate cancer was observed among individuals with the CYP1A1 GA + GG genotype. CYP1A1 GA + GG genotypes were associated with increased cancer risk in low (OR, 2.05; 95% CI, 1.19-3.63), moderate (OR, 1.72; 95% CI, 1.07-2.76), and high (OR, 2.86; 95% CI, 1.83-4.47) HAA intake groups. PMID: 29165164
    22. rs4886605 and rs4646903 are associated with increased CAD risk, but rs1048943 is associated with decreased CAD risk; we also found gene-gene interaction between rs4886605 and rs4646903 and gene-environment interaction between rs4886605 and smoking. PMID: 28513235
    23. CYP1A1 has an oncogenic role in prostate cancer via promoter hypomethylation that is influenced by tobacco smoking PMID: 27203547
    24. Utilising a three-dimensional lymph endothelial cell (LEC) monolayer & MDA-MB231 breast cancer cell spheroid co-culture model in combination with knock-down approach allowed elucidation of the molecular/biochemical basis of AHR/CYP1A1-induced tumour breaching through the LEC barrier. PMID: 28171546
    25. This meta-analysis demonstrated CYP1A1 rs1048943 A>G may increase the susceptibility to colorectal cancer (CRC) instead of rs4646903 T> C. This conclusion suggested CYP1A1 may contribute to the pathogenesis of CRC. [meta-analysis] PMID: 27384991
    26. The Rs4646903 polymorphism of CYP1A1 is Associated with Recurrent Pregnancy Loss, especially among South Asians. PMID: 28620991
    27. We demonstrated that infants whose mothers smoked during pregnancy with the combination of AHR, CYP1A1, and XRCC1 polymorphisms had lower birth size. PMID: 27592400
    28. genetic variations in STX1B, DNMT3A and CYP1A1 have roles in influencing warfarin maintenance dose PMID: 27740732
    29. CYP1A1 rs2606345 influences response to anticonvulsants in North Indian women with epilepsy by lowering CYP1A1 expression. PMID: 26951882
    30. This is the first report providing evidence of hyperoxia-mediated transcriptional activation of the human CYP1A1 promoter in newborn mice, and this in conjunction with decreased lung injury, suggests that these phenomena have important implications for bronchopulmonary dysplasia. PMID: 29101037
    31. Review/Meta-analysis: CYP1A1 T3801C and A2455G variants might not be risk alleles for breast cancer susceptibility in Chinese individuals. PMID: 28164497
    32. These findings identify a role for pattern recognition receptor (PRR) signaling in regulating the AHR response through selective down-regulation of Cyp1 expression in human monocytes and macrophages. PMID: 29018148
    33. AHR, CYP1A1, CYP1A2, or CYP1B1 variants associated with head-and-neck squamous cell carcinoma risk in smokers. PMID: 27894333
    34. Our meta-analysis revealed strong association of rs1048943 in CYP1A1, but a suggestive association of deletion polymorphisms in GSTT1 and GSTM1 with lung cancer, which provides a comprehensive insight on the overall effect of the polymorphic variants, reported in various case-control studies on Indian population, on the risk of lung cancer development PMID: 29076184
    35. Increased expression of CYP1A1 is associated with ovarian/peritoneal endometriotic lesions. PMID: 27012269
    36. CYP1A1m2 polymorphism showed an increased risk in the population leading to cervical cancer PMID: 28433806
    37. mtDNA content, methylation of specific loci of mtDNA, and CYP1A1 methylation in placental tissue may serve as molecular signatures for the association between gestational tobacco smoke exposure and low birth weight. PMID: 28052772
    38. Studied diosmetin effect on signal pathway and apoptosis in HepG2 cells. Dios-induced cell apoptosis could be reversed by p53 blockade and the effects of Dios on cellular P53 and CYP1A1/CYP1A2 proteins levels were examined. Found P53, CYP1A1, and CYP1A2 proteins were up-regulated by diosmetin; p53 downregulation by pifithrin caused inhibition of apoptosis. PMID: 28245756
    39. individuals with CYP1A1m13801CC and 3801TC genotypes independently and in the presence of alcohol and nevirapine usage is likely to be associated with increased risk of development of antiretroviral-associated hepatotoxicity, its severity, and advancement of disease PMID: 28370504
    40. the CYP1A1 polymorphisms are potential risk factors for digestive tract cancer. PMID: 27061602
    41. was observed that the variant genotypes of GSTM1, GSTT1, GSTP1 and CYP1A1 did not significantly increase the risk of cervical cancer (CC), however, statistically significant increased risk was observed for women who used wood for cooking and had GSTM1 (null) genotype. PMID: 28361858
    42. CYP1A1*2A was not associated with Colorectal Cancer(CRC)development (OR = 1.566; 95% confidence interval [CI] = 0.90-2.73; p = 0.12); C allele-carrying individuals who had smoked within the past 5 years had a significant risk of CRC (OR = 2.28; 95% CI = 1.07-4.86; p = 0.043). PMID: 27243113
    43. CYP1A1 polymorphisms were not associated with sporadic colorectal neoplasms. PMID: 28018104
    44. Allele frequency of the CYP1A1 3801T>C polymorphism does not correlate with semen production as determined by sperm counts or sperm function as determined by fertilization rates with ICSI. PMID: 27117375
    45. data indicate that CYP1A1 I462V allele confers the least carcinogen-associated genotoxicity, compared to CYP1A1; however, results vary depending on the chemical carcinogen and the genotoxic endpoint PMID: 28283091
    46. By sequencing Apc and Ctnnb1 genes, we found that most PhIP-induced small intestinal tumors in obese mice carried only a single heterozygous mutation in Apc . Our findings demonstrate that PhIP-induced small intestinal carcinogenesis in hCYP1A-db/db mice is promoted by obesity and involves Apc mutation and inactivation by DNA hypermethylation PMID: 27207656
    47. The rapid activation of the Ah receptor and CYP1A1 protein expression by higher chlorinated polychlorinated naphthalene point to their toxicity; however, it is not sufficient for potential carcinogenicity. PMID: 27501765
    48. The polymorphism of CYP1A1*4 is associated with esophageal squamous cell carcinoma risk. PMID: 26455829
    49. Our results showed that 3 single nucleotide polymorphisms of ACE rs4340, IL-6 rs1800795, and NOS3 rs1799983 were significantly associated with the risks of mycoplasma pneumoniae pneumonia (MPP), while no differences were observed in genotype frequencies of GSTM1 and CYP1A1. The combinations of ACE/NOS3/CYP1A1 and ACE /NOS3 contribute to the genetic susceptibility of MPP in Chinese children. PMID: 28403117
    50. the CYP1A1 (rs4646422) polymorphism might be involved in gastric carcinogenesis among the Japanese population. PMID: 27062139

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Cytoplasm.
  • 蛋白家族：
    Cytochrome P450 family
  • 组织特异性：
    Lung, lymphocytes and placenta.
  • 数据库链接：

    HGNC: 2595

    OMIM: 108330

    KEGG: hsa:1543

    STRING: 9606.ENSP00000369050

    UniGene: Hs.72912