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  4. Recombinant Human Cysteine protease ATG4A (ATG4A)

Recombinant Human Cysteine protease ATG4A (ATG4A)

  • 货号：
    CSB-YP845174HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP845174HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP845174HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP845174HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP845174HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    AI627006; APG4 autophagy 4 homolog A; Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4 autophagy related 4 homolog A; ATG4A; ATG4A_HUMAN; Atg4al; AUT like 2 cysteine endopeptidase; AUT-like 2 cysteine endopeptidase; Autl2; Autophagin 2; Autophagin-2; Autophagy related 4A cysteine peptidase; Autophagy related cysteine endopeptidase 2; Autophagy related protein 4 homolog A; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; AV169859; Cysteine protease ATG4A; hAPG4A; MGC107179
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-398
  • 氨基酸序列
    MESVLSKYED QITIFTDYLE EYPDTDELVW ILGKQHLLKT EKSKLLSDIS ARLWFTYRRK FSPIGGTGPS SDAGWGCMLR CGQMMLAQAL ICRHLGRDWS WEKQKEQPKE YQRILQCFLD RKDCCYSIHQ MAQMGVGEGK SIGEWFGPNT VAQVLKKLAL FDEWNSLAVY VSMDNTVVIE DIKKMCRVLP LSADTAGDRP PDSLTASNQS KGTSAYCSAW KPLLLIVPLR LGINQINPVY VDAFKECFKM PQSLGALGGK PNNAYYFIGF LGDELIFLDP HTTQTFVDTE ENGTVNDQTF HCLQSPQRMN ILNLDPSVAL GFFCKEEKDF DNWCSLVQKE ILKENLRMFE LVQKHPSHWP PFVPPAKPEV TTTGAEFIDS TEQLEEFDLE EDFEILSV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
  • 基因功能参考文献：
    1. An intron SNP rs807185 in ATG4A might be a protective factor for lung cancer. PMID: 26061994
    2. ATG4A promotes the metastasis of gastric cancer cells via the Notch signaling pathway, which is an autophagy-independent mechanism PMID: 27276686
    3. The structure and regulatory machinery of Atg4a [review] PMID: 28253956
    4. We conclude that miR-24-3p regulates autophagy by targeting ATG4A PMID: 25426560
    5. The autophagy regulator ATG4A was found to be essential for the maintenance of a sub-population with cancer stem cell properties and to regulate breast cancer cell tumourigenicity. PMID: 24229464
    6. The demonstration that expression of ATG4A in cells increased stem properties provided an indication of its biological function. Hypomethylation of ATG4A and HIST1H2BN in ovarian tumor-initiating cells predicts poor prognosis for ovarian cancer patients. PMID: 24256813
    7. no association between SNP rs807185 and the presence of granulomas in Crohn's disease PMID: 21122541
    8. statistical analysis of APG4A gene confirmed the characterisation of each protein-coding gene present in human genome Xq22.1 PMID: 21297227
    9. Atg4B possessed the broadest spectrum against all substrates, followed by Atg4A for ATG8 substrates PMID: 21177865
    10. HsApg4A cleaves the COOH terminus of GATE-16 PMID: 12473658
    11. The cysteine protease HsAtg4 is identified as a direct target for oxidation by hydrogen peroxide, and a cysteine residue located near the HsAtg4 catalytic site as critical for this regulation. PMID: 17347651

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C54 family
  • 数据库链接：

    HGNC: 16489

    OMIM: 300663

    KEGG: hsa:115201

    STRING: 9606.ENSP00000361306

    UniGene: Hs.8763