Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Human Cystatin-M (CST6)

Recombinant Human Cystatin-M (CST6)

  • 货号：
    CSB-YP623015HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP623015HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP623015HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP623015HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP623015HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CST6
  • Uniprot No.：
  • 别名：
    CST 6; CST6; Cystatin 6; Cystatin E; Cystatin E/M; Cystatin M; Cystatin M/E; Cystatin-6; Cystatin-E; Cystatin-M; Cystatin6; CystatinE; CystatinM; Cysteine proteinase inhibitor; CYTM_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    29-149
  • 氨基酸序列
    RP QERMVGELRD LSPDDPQVQK AAQAAVASYN MGSNSIYYFR DTHIIKAQSQ LVAGIKYFLT MEMGSTDCRK TRVTGDHVDL TTCPLAAGAQ QEKLRCDFEV LVVPWQNSSQ LLKHNCVQM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    High affinity inhibitor for cathepsin L, cathepsin L2 (cathepsin V), and legumain. Involved in the regulation of epidermal cornification, and hair follicle morphogenesis and maintenance.
  • 基因功能参考文献：
    1. High CST6 expression is associated with lymph-node metastasis in Triple-Negative Breast Cancer. PMID: 29530995
    2. Results provide evidence that cystatin E/M is a tumor suppressor gene which plays an important role in the regulation of NF-kappaB. PMID: 27090639
    3. Low CST6 expression is associated with breast cancer. PMID: 24742492
    4. Developed a Methylation-Sensitive High Resolution Melting Analysis (MS-HRMA) assay for the investigation of CST6 promoter methylation in breast cancer patients.CST6 promoter was methylated in 39/80 of FFPEs. PMID: 23088560
    5. The CST6 promoter is highly methylated in circulating cell-free DNA of breast cancer patients, but not in healthy individuals. PMID: 23006792
    6. Data show a regulatory role of cystatin E/M in controlling both intra- and extracellular legumain activity. PMID: 22902879
    7. The ectopic expression of CST6 in cancer cells rescued mice from overt osteolytic metastasis and deaths in the animal study, while CST6 knockdown markedly enhanced cancer cell bone metastasis and shortened animal survival. PMID: 22688893
    8. An association between the quantity of CST6 methylation and the expression statuses of estrogen receptor, progesterone receptor, and HER4 in tumor tissues was found PMID: 21092257
    9. the level of cystatin E/M regulates legumain activity and hence the invasive potential of human melanoma cells PMID: 20074384
    10. CST6 is not a major gene contributing to type 1 and 2 harlequin ichthyosis PMID: 12839564
    11. Cystatin M suppresses the malignant phenotype of MDA-MB-435S cells. PMID: 14676833
    12. Complete loss of expression of cystatin M was observed in two of three stage IV breast cancer patients. Immunohistochemical studies confirmed that expression of cystatin M in IDCs was partially or completely lost. PMID: 15466187
    13. This study thus provides the first evidence that CST6 plays a role in the modulation of genes, particularly, genes that are highly relevant to breast cancer progression. PMID: 16356477
    14. Identification of CTSV & CTSL as targets for cystatin M/E, their (co)-expression in the stratum granulosum of skin, and activity of CTSL toward transglutaminase 3 strongly imply an important role for them in the differentiation process of human epidermis. PMID: 16565075
    15. Tumors displaying CST6 gene methylation, display methylation in both ductal carcinoma in situ and invasive breast carcinoma lesions and reduced expression of cystatin M in these tumors was confirmed by immunohistochemistry PMID: 16912163
    16. strong link between CST6 promoter hypermethylation and loss of CST6 expression in breast cancer cell lines PMID: 17043665
    17. Cystatin M may encode a novel epigenetically inactivated candidate tumor suppressor gene. PMID: 17099723
    18. Methylation-dependent epigenetic silencing of CST6 represents an important mechanism for loss of CST6 during breast tumorigenesis and/or progression to metastasis. PMID: 17540367
    19. cystatin E/M is a cervical cancer suppressor gene and that the gene is inactivated by somatic mutations and promoter hypermethylation. PMID: 18506750
    20. results demonstrate that epigenetic silencing of CST6 is frequent in adult and pediatric brain tumors and occurs in TICs, which are thought to give rise to the tumor PMID: 18607344
    21. CST6, CXCL14, DHRS3, and SPP1 are regulated by BRAF signaling and may play a role in papillary thyroid carcinoma pathogenesis PMID: 18676742
    22. These findings imply that CST6 is likely to involve in the proliferation and survival of pancreatic cancer probably through its proteinase inhibitory activity, and it is a promising molecular target for development of new therapeutic strategies for PDAC PMID: 18754876
    23. The cysteine protease inhibitor cystatin M/E is a key regulator of a biochemical pathway that leads to epidermal terminal differentiation. PMID: 19005484
    24. Cystatin M (CST6) tumor suppressor gene is concurrently down-regulated with other loci in breast epithelial cells cocultured with cancer-associated fibroblasts. PMID: 19074894
    25. cystatin M/E has a role in skin barrier formation and a potential role as a tumor suppressor gene [review] PMID: 19262604
    26. Results suggest that the downregulation of the CST6 gene is associated with promoter histone modifications and that this association plays an important role in prostate cancer progression during the invasive and metastatic stages of the disease. PMID: 19503093
    27. Methylation of cystatin M promoter is associated with breast cancer. PMID: 19551853

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Cystatin family
  • 组织特异性：
    Restricted to the stratum granulosum of normal skin, the stratum granulosum/spinosum of psoriatic skin, and the secretory coils of eccrine sweat glands. Low expression levels are found in the nasal cavity.
  • 数据库链接：

    HGNC: 2478

    OMIM: 601891

    KEGG: hsa:1474

    STRING: 9606.ENSP00000311313

    UniGene: Hs.139389