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  4. Recombinant Human Cyclin-A2 (CCNA2)

Recombinant Human Cyclin-A2 (CCNA2)

  • 货号：
    CSB-YP004805HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004805HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004805HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004805HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CCN1; CCNA; Ccna2; CCNA2_HUMAN; Cyclin A2; Cyclin-A; Cyclin-A2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-432
  • 氨基酸序列
    MLGNSAPGPA TREAGSALLA LQQTALQEDQ ENINPEKAAP VQQPRTRAAL AVLKSGNPRG LAQQQRPKTR RVAPLKDLPV NDEHVTVPPW KANSKQPAFT IHVDEAEKEA QKKPAESQKI EREDALAFNS AISLPGPRKP LVPLDYPMDG SFESPHTMDM SIILEDEKPV SVNEVPDYHE DIHTYLREME VKCKPKVGYM KKQPDITNSM RAILVDWLVE VGEEYKLQNE TLHLAVNYID RFLSSMSVLR GKLQLVGTAA MLLASKFEEI YPPEVAEFVY ITDDTYTKKQ VLRMEHLVLK VLTFDLAAPT VNQFLTQYFL HQQPANCKVE SLAMFLGELS LIDADPYLKY LPSVIAGAAF HLALYTVTGQ SWPESLIRKT GYTLESLKPC LMDLHQTYLK APQHAQQSIR EKYKNSKYHG VSLLNPPETL NL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.
  • 基因功能参考文献：
    1. High CCNA2 expression is associated with hepatocellular carcinoma progression. PMID: 30106440
    2. Study demonstrates that cyclin A2 interacts with actin and RhoA during mitosis, and that cyclin A2 depletion induces a dramatic decrease in active RhoA in mitosis suggesting that cyclin A2 participation in RhoA activation in late mitosis. PMID: 27279564
    3. Bisphosphonate inhibits the expression of cyclin A2 at the transcriptional level in normal human oral keratinocytes. PMID: 28713904
    4. the presented evidence suggested that WA can inhibit HCC cell proliferationand tumorigenesis through miR-22-repressed CCNA2, which was at least partially through FXR regulation PMID: 27738335
    5. Findings suggest that ERK1/2-mediated Cdk2/cyclin A signaling pathway is involved in 7-hydroxy-5,4'-dimethoxy-2-arylbenzofuran (Ary) - induced G1/S-phase arrest. PMID: 27259234
    6. some cancer cells are defective for efficient interaction between p27 and CycA-CDK complex due to qualitative alteration(s) PMID: 29098944
    7. This study shows that cocaine induces significant increases in both the astrocytic expression of cyclin A2 and the proliferation of primary human astrocytes. PMID: 27834787
    8. Low CCNA expression is associated with lung cancer progression. PMID: 27694898
    9. inhibition of PDK4 activity in Hepatocellular carcinoma cells increased cyclin E1, cyclin A2, and E2F1 proteins. PMID: 28003426
    10. An increase of cyclin A2 expression was identified for the first time in a case of splenic diffuse red pulp small B-cell lymphoma. PMID: 27761608
    11. Consistent with these findings, a genome-scale pooled RNA interference screen revealed that toxic doses of MK-1775 are suppressed by CDK2 or Cyclin A2 knockdown. These findings support G2 exit as the more significant effect of Wee1 inhibition in pancreatic cancers. PMID: 26890070
    12. Clioquinol suppressed cell cycle progression in the S-phase in SMMC-7721 hepatoma cells via the p21, p27-cyclin E,A/Cdk2 pathway. PMID: 26677902
    13. On analyzing cyclin A and B1 (CCNA and CCNB1) expression, positive staining in 90% cases of PTC were observed. The study revealed a significant difference in expression of cyclins A and B1 between classic and non-classic variants of PTC. PMID: 26706989
    14. Study describes a positive feedback loop centred on cyclin A2-Cdk2 inhibition of interphase APC/C-Cdc20 to allow further cyclin A2 accumulation and mitotic entry. PMID: 26960431
    15. PIWIL2 has a role in promoting progression of non-small cell lung cancer by inducing CDK2 and Cyclin A expression PMID: 26373553
    16. Cyclin A2-binding function of pUL21a contributes to the maintenance of a cell cycle state conducive for the completion of the human cytomegalovirus (HCMV) replication cycle. PMID: 25393019
    17. HDAC10 regulates cyclin A2 expression by deacetylating histones near the let-7 promoter. PMID: 26240284
    18. Results suggest that cyclin A2 overexpression may drive cancer by promoting AKT-dependent proliferation and survival. PMID: 24795023
    19. for the first time, we demonstrated that CTD induced G2/M phase arrest via the inhibition of Cdc25c and cyclin A and induced apoptosis was through death receptor (extrinsic), intrinsic (mitochondria) and ER stress pathways in A375. S2 cells. PMID: 25340978
    20. CCN2 is a regulator of the transition through cytokinesis during terminal erythropoiesis. PMID: 25615569
    21. CCNA2 is a biomarker for the prognosis of ER+ breast cancer and monitoring of tamoxifen efficacy PMID: 24622579
    22. our data showed that bile acid-activated FXR stimulates miR-22-silenced CCNA2, a novel pathway for FXR to exert its protective effect in the gastrointestinal tract. PMID: 25596928
    23. Expression of cycle marker cyclin A1 differs between benign and malignant papillary breast lesions. PMID: 25501285
    24. Cyclin A2 and its associated kinase (cdk2) activity are required for optimal induction of progesterone receptor target genes in breast cancer cells. PMID: 25220500
    25. It is concluded that non-response to everolimus is characterized by increased cdk2/cyclin A, driving RCC cells into the G2/M-phase. VPA hinders everolimus non-response by diminishing cdk2/cyclin A PMID: 24935000
    26. cyclin A expression predicted 100% of the patients at risk for metastasis of rectal neuroendocrine tumors. PMID: 24824027
    27. The aim of the present study was to evaluate the prognostic value of the proliferation factors mitotic activity index (MAI), phosphohistone H3 (PPH3), cyclin B1, cyclin A and Ki67, alone and in combinations. PMID: 24324728
    28. show that cyclin A is an independent prognostic factor in endometrioid adenocarcinoma PMID: 24519066
    29. level unchanged in endometrial cancer PMID: 23815208
    30. APPOLON to be a novel regulator of mitotic CYCLIN A degradation independent of SAC. PMID: 24302728
    31. Data indicate that the major tegument 150-kDa phosphoprotein (pp150) of human cytomegalovirus (HCMV) binds to cyclin A2 via a functional RXL/Cy motif resulting in its cyclin A2-dependent phosphorylation. PMID: 24101496
    32. HDAC3 regulates cyclin A stability. PMID: 23760262
    33. Cyclin A creates a cellular environment that promotes microtubule detachment from kinetochores in prometaphase to ensure efficient error correction and faithful chromosome segregation PMID: 24013174
    34. We further detected that reduced level of GRK2 induced a small cell cycle arrest at G2/M phase by enhancing the expression of cyclin A PMID: 23460259
    35. Overexpression of cyclin A2 is associated with stage I non-small cell lung cancer. PMID: 23115005
    36. human papillomavirus E4 proteins can interact with cyclin A and cdk2, which may contribute to viral manipulation of the host cell cycle. PMID: 23065011
    37. The expression of cell proliferation markers cyclin A and p27 are independent prognostic factors in patients with esophageal squamous cell carcinoma. PMID: 21762277
    38. The study has revealed that some highly differentiated human tissues express an intron-retaining cyclin A2 mRNA that induced a G1/S block in vitro. PMID: 22745723
    39. Human cytomegalovirus IE1/2 expression was downregulated by cyclin A2, CDK1 and CDK2. PMID: 22718829
    40. cyclin A and cyclin B1 are useful markers in the distinction of benign and malignant thyroid tumors PMID: 21264543
    41. GC box was responsible for the cyclic activity of human CKAP2 promoter through the phosphorylation of Sp1, possibly by Cyclin A/Cdk complex. PMID: 22465120
    42. Data demonstrated that in addition to galectin-3, HK III and cyclin A profiles could be important biomarkers in predicting malignancy in follicular thyroid nodules. PMID: 17868400
    43. Cyclin A2 negatively controls cell motility by promoting RhoA activation, thus demonstrating a novel Cyclin A2 function in cytoskeletal rearrangements and cell migration. PMID: 22232705
    44. NF-Y binds to CCAAT sequences in the Cyclin A promoter, as well as to those in the promoters of cell cycle G2 regulators such as CDC2, Cyclin B and CDC25C. PMID: 21871181
    45. These data demonstrate that B55alpha acts to antagonize Cyclin A/Cdk-dependent activation of FoxM1, to ensure that FoxM1 activity is restricted to the G(2) phase of the cell cycle. PMID: 21813648
    46. The current results suggested that an SNP (rs769236) at the promoter of CCNA2 may be associated significantly with increased risk of colon, liver, and lung cancers. PMID: 21858804
    47. The expression of mRNA and protein of Cyclin A in hypertrophic scar changes from high level to low level as the hypertrophic scar develops, while the expression of P21cip1 changes from low level to high level. PMID: 21046779
    48. Low p16(Ink4a) expression, overexpression of p53, cytoplasmic p27(Kip1), and Cyclin A are predictive markers for shorter overall survival in ovarian carcinomas. PMID: 21464733
    49. cyclin A was strongly associated with the expression of cyclin E in invasive ductal breast carcinoma PMID: 21472690
    50. Cdc20 requires APC3 and APC8 to bind and activate the APC/C when the spindle assembly checkpoint is satisfied, but only APC8 when active, and APC10 is crucial for the destruction of cyclin B1 and securin, but not cyclin A PMID: 21336306

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Cyclin family, Cyclin AB subfamily
  • 数据库链接：

    HGNC: 1578

    OMIM: 123835

    KEGG: hsa:890

    STRING: 9606.ENSP00000274026

    UniGene: Hs.58974