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  4. Recombinant Human Core histone macro-H2A.1 (H2AFY)

Recombinant Human Core histone macro-H2A.1 (H2AFY)

  • 货号：
    CSB-YP010098HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010098HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010098HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010098HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010098HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Core histone macro h2a.1; Core histone macro-H2A.1; H2A histone family member Y ; H2A.y; H2A/y; H2AF12M; H2AFJ; H2afy; H2AY_HUMAN; Histone H2A.Y; Histone macroH2A1; Histone macroH2A1.1; Histone macroH2A1.2; Macroh2a1; MACROH2A1.1; MacroH2A1.2; Medulloblastoma antigen MU MB 50.205 ; Medulloblastoma antigen MU-MB-50.205; mH2a; mH2A1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-372
  • 氨基酸序列
    MSSRGGKKKS TKTSRSAKAG VIFPVGRMLR YIKKGHPKYR IGVGAPVYMA AVLEYLTAEI LELAGNAARD NKKGRVTPRH ILLAVANDEE LNQLLKGVTI ASGGVLPNIH PELLAKKRGS KGKLEAIITP PPAKKAKSPS QKKPVSKKAG GKKGARKSKK KQGEVSKAAS ADSTTEGTPA DGFTVLSTKS LFLGQKLNLI HSEISNLAGF EVEAIINPTN ADIDLKDDLG NTLEKKGGKE FVEAVLELRK KNGPLEVAGA AVSAGHGLPA KFVIHCNSPV WGADKCEELL EKTVKNCLAL ADDKKLKSIA FPSIGSGRNG FPKQTAAQLI LKAISSYFVS TMSSSIKTVY FVLFDSESIG IYVQEMAKLD AN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin.; Binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. Increases the expression of genes involved in redox metabolism, including SOD3.; Represses SOD3 gene expression.
  • 基因功能参考文献：
    1. This article points to a splicing-regulated, proto-oncogenic role for the macroH2A1.2 variant and suggests manipulation of macroH2A1.2 expression as a potential therapeutic means to interfere with tumorigenesis. PMID: 29249653
    2. The Skp2-mH2A1-CDK8 axis has a critical role in breast cancer development via dysregulation of the G2/M transition, polyploidy, cell growth dysregulation, and loss of tumor suppression. PMID: 25818643
    3. We show that composite nucleosomes containing mH2A and NRF-1 are stably positioned on gene regulatory regions and can buffer transcriptional noise associated with antiviral responses PMID: 25959814
    4. High MacroH2A1 expression is associated with epigenetic markers for activation of lipogenic genes in fat-induced steatosis. PMID: 25526730
    5. macroH2A1.1 expression correlates with poor survival of triple-negative breast cancer patients PMID: 24911873
    6. MacroH2A1.1 and PARP-1 cooperate to regulate transcription by promoting CBP-mediated H2B acetylation. PMID: 25306110
    7. MacroH2A1 specifically recruits PELP1 to the promoters of macroH2A1 target genes, but macroH2A1 occupancy occurs independent of PELP1. This recruitment allows macroH2A1 and PELP1 to cooperatively regulate gene expression outcomes. PMID: 24752897
    8. The results demonstrate that macroH2A1 is a new factor involved in the regulation of rDNA transcription. PMID: 24071584
    9. macro histone variants (macroH2A) are expressed at low levels in stem cells and are up-regulated during differentiation PMID: 23595991
    10. Both macroH2A1 isoforms may play a role in hepatocellular carcinoma pathogenesis and may be considered as novel diagnostic markers for human hepatocellular carcinoma. PMID: 23372727
    11. MacroH2A1 splicing isoforms differentially regulate the transcription of a set of genes involved in redox metabolism. PMID: 23022728
    12. The histone variant macroH2A1.1 is recruited to DNA double-strand breaks through a mechanism involving PARP1. PMID: 23031826
    13. Within the macro domain of mH2A1.2, a trinucleotide insertion (-EIS-) sequence not found in mH2A1.1 was essential for the interaction between HER-2 and mH2A1.2 as well as mH2A1.2-induced HER-2 expression and cell proliferation. PMID: 22589551
    14. ATRX (alpha-thalassemia/MR, X-linked) is a novel macroH2A-interacting protein PMID: 22391447
    15. H2AFY is specifically overexpressed in the blood and frontal cortex of patients with Huntington disease compared with controls PMID: 21969577
    16. macroH2A can play either a positive or negative role in transcriptional regulation in a context-dependent manner. Additionally, macroH2A has been linked to the control of the cell cycle and cell proliferation. PMID: 20543561
    17. significant inverse correlation between mH2A and CDK8 expression levels exists in melanoma patient samples PMID: 21179167
    18. distributed during the maintainance phase of x inactivation throughout cell cycle PMID: 12082075
    19. MACROH2A1 deposition is regulated by the CULLIN3/SPOP ligase complex and is actively involved in stable X inactivation. PMID: 15897469
    20. A specific interaction between mH2A1.1 and PARP-1 was demonstrated and found to be associated with inactivation of PARP-1 enzymatic activity. PMID: 17158748
    21. multiple short sequences dispersed along the macroH2A1 histone domain individually supported enrichment on the inactive X chromosome when introduced into H2A PMID: 17570398
    22. A phosphorylation site, S137ph, was identified in mH2A1;S137ph is enriched in mitosis. PMID: 18227505
    23. expression of histone macroH2A1.1 and macroH2A2 predicts lung cancer recurrence. PMID: 19648962
    24. show that distinct macrodomains, including those of histone macroH2A1.1, are recruited to sites of PARP1 activation induced by laser-generated DNA damage. PMID: 19680243
    25. an unexpected role for macroH2A1 in the escape from heterochromatin-associated silencing and the enhancement of autosomal gene transcription PMID: 20008927

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 4740

    OMIM: 610054

    KEGG: hsa:9555

    STRING: 9606.ENSP00000423563

    UniGene: Hs.420272