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  4. Recombinant Human Cis-aconitate decarboxylase (ACOD1)

Recombinant Human Cis-aconitate decarboxylase (ACOD1)

  • 货号：
    CSB-YP011825HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011825HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011825HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011825HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ACOD1
  • Uniprot No.：
  • 别名：
    ACOD1; Aconitate decarboxylase 1; Aconitate decarboxylase; CAD; Cis aconitate decarboxylase; Immune responsive gene 1 protein homolog; Immune-responsive gene 1 protein homolog; Immunoresponsive 1 homolog (mouse); Irg1; IRG1_HUMAN; OC116; OPTB1; TCTIRC7
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-481
  • 氨基酸序列
    MMLKSITESF ATAIHGLKVG HLTDRVIQRS KRMILDTLGA GFLGTTTEVF HIASQYSKIY SSNISSTVWG QPDIRLPPTY AAFVNGVAIH SMDFDDTWHP ATHPSGAVLP VLTALAEALP RSPKFSGLDL LLAFNVGIEV QGRLLHFAKE ANDMPKRFHP PSVVGTLGSA AAASKFLGLS STKCREALAI AVSHAGAPMA NAATQTKPLH IGNAAKHGIE AAFLAMLGLQ GNKQVLDLEA GFGAFYANYS PKVLPSIASY SWLLDQQDVA FKRFPAHLST HWVADAAASV RKHLVAERAL LPTDYIKRIV LRIPNVQYVN RPFPVSEHEA RHSFQYVACA MLLDGGITVP SFHECQINRP QVRELLSKVE LEYPPDNLPS FNILYCEISV TLKDGATFTD RSDTFYGHWR KPLSQEDLEE KFRANASKML SWDTVESLIK IVKNLEDLED CSVLTTLLKG PSPPEVASNS PACNNSITNL S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages. Involved in antiviral response following infection by flavivirus in neurons: ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes. Plays a role in the embryo implantation.
  • 基因功能参考文献：
    1. In conclusion, we found that IRG1 promoted MHC I level in macrophages membrane via ROS-STAT1/3-TAP1 pathway. Besides, IRG1 could activate the pentose phosphate pathway to promote production of ROS depending on NADPH oxidases. PMID: 28477473
    2. findings suggest that polymorphisms in the immunoresponsive gene 1 (IRG1) gene are associated with the immune response to hepatitis B vaccination; observed that IRG1 inhibited the HBV life cycle and that IRG1 rs17385627 allele 'A' was more effective than rs17385627 allele 'T' at eliminating HBV in HepG2.2.15 cell PMID: 28004399
    3. Respiratory syncytial virus infection induced IRG1 expression in human A549 cells and in the lung tissues of respiratory syncytial virus -infected mice and respiratory syncytial virus infection or IRG1 overexpression promoted reactive oxygen species production. PMID: 27252532
    4. IRF1 is a transcriptional regulator of IRG1 in human macrophages. PMID: 26872335
    5. We identified IRF1 as a transcriptional regulator of IRG1 expression in both human and mouse macrophages under inflammatory conditions. PMID: 26872335
    6. IRG1/CAD associates with mitochondria in human macrophages under inflammatory conditions such as LPS and/or IFNg. PMID: 26872335
    7. Taken together, the results show that IRG1 is expressed by human monocytes, macrophages and dendritic cells and that it is rapidly induced in both human and murine macrophages under inflammatory conditions such as LPS, TNFa and IFNg. PMID: 26872335
    8. Findings revealed that IRG1 is a candidate oncogene that is amplified in glioma and is involved in novel mechanisms that influence glioma pathogenesis. PMID: 25216059
    9. inducible IRG1 promotes endotoxin tolerance by increasing A20 expression through ROS, indicating a new molecular mechanism regulating hypoinflammation of sepsis and endotoxin tolerance. PMID: 23609450
    10. IRG1 links cellular metabolism with immune defense by catalyzing itaconic acid production PMID: 23610393
    11. Irg1 gene encodes an enzyme synthesizing itaconic acid from the TCA cycle intermediate cisaconitate. Furthermore, our data also show that IRG1 mediated itaconic acid production contributes to the antimicrobial activity of macrophages. PMID: 23610393
    12. Results indicated that the IRG1 gene is differentially expressed in human fetal PBMCs and LPS-stimulated adult PBMCs. PMID: 21424586

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  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    PrpD family
  • 组织特异性：
    Expressed in LPS-tolerized macrophages (at protein level). Expressed in peripheral blood mononuclear cells (PBMCs), microglia and macrophage cells.
  • 数据库链接：

    HGNC: 33904

    OMIM: 615275

    KEGG: hsa:730249

    STRING: 9606.ENSP00000366682

    UniGene: Hs.160789