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  4. Recombinant Human Chromobox protein homolog 3 (CBX3)

Recombinant Human Chromobox protein homolog 3 (CBX3)

  • 货号：
    CSB-YP613383HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP613383HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP613383HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP613383HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP613383HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CBX3
  • Uniprot No.：
  • 别名：
    CBX 3; CBX3; CBX3_HUMAN; Chromobox homolog 3 (HP1 gamma homolog; Drosophila); Chromobox homolog 3; Chromobox protein homolog 3; GAMMA; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; Heterochromatin protein HP1 gamma; HP1 gamma; HP1 gamma homolog; HP1Hs gamma; Modifier 2 protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-183
  • 氨基酸序列
    MASNKTTLQK MGKKQNGKSK KVEEAEPEEF VVEKVLDRRV VNGKVEYFLK WKGFTDADNT WEPEENLDCP ELIEAFLNSQ KAGKEKDGTK RKSLSDSESD DSKSKKKRDA ADKPRGFARG LDPERIIGAT DSSGELMFLM KWKDSDEADL VLAKEANMKC PQIVIAFYEE RLTWHSCPED EAQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs).
  • 基因功能参考文献：
    1. c-Myc was capable of upregulating HP1gamma by directly binding to the E-box element in the first intron of HP1gamma gene, and the upregulated HP1gamma, in turn, repressed the expression of miR-451a by enhancing H3K9 methylation at the promoter region of miR-451a. PMID: 28967902
    2. CBX3 was overexpressed in pancreatic adenocarcinoma, which was associated with poor prognosis. CDK1 knockdown attenuated the cell cycle transition, proliferation and invasion of CBX3-overexpressing PAAD cells. PMID: 29903985
    3. CBX3 serves as a positive regulator of aerobic glycolysis via suppressing of the FBP1 in pancreatic cancer cells. PMID: 29678579
    4. HP1gamma is present in the nucleus and the cytoplasm interacting with actin, a protein complex that may exert different functions depending on its subcellular localization. PMID: 29208528
    5. CBX3 has a role in promoting tumor proliferation by regulating G1/S phase via p21 downregulation and associates with poor prognosis in tongue squamous cell carcinoma PMID: 29462646
    6. The deletion of CBX3 directly enforces the expression of CDK6 and p21. PMID: 28193906
    7. These data indicate that HP1-gamma is a novel epigenetic repressor of epsilon-globin gene expression. PMID: 28154185
    8. these data underscore the importance of IDRs 1, 2, and 3 in defining the structural and dynamic properties of HP1gamma, discoveries that have both mechanistic and potentially biomedical relevance. PMID: 26680990
    9. In contrast to HP1alpha, HP1gamma could not recognize H3K9me3 in extended nucleosomes. PMID: 26319017
    10. this study identified miR-30a as a tumor-suppressive microRNA that targets HP1gamma in vitro and in vivo to specifically suppress the growth of colorectal cancer in mouse xenograft models. PMID: 26333808
    11. BARD1 interacts with H3K9me2 through HP1 and plays a critical role in retention of a BRCA1/BARD1 complex at the site of DNA damage. PMID: 25634209
    12. CBX3 and CRABP2 expression was markedly increased in lung cancer tissues PMID: 24751108
    13. our data suggested that HP1gamma plays an important role in promoting non-small cell lung cancer PMID: 24981246
    14. compartmentalized and total HP1gamma protein expression is increased in PCa and that expression correlates with clinical outcomes better than Gleason score. PMID: 23522301
    15. HP1gamma affects the spontaneous differentiation potential of P19 cells, and might play major roles in the decision of cell fates in pluripotent stem cells. PMID: 23313480
    16. Identification of CBX3 and ABCA5 as putative biomarkers for tumor stem cells in osteosarcoma. PMID: 22870217
    17. Loss of CBX3 results in global RNA processing defect. PMID: 22684280
    18. The Cbx3 chromodomain binds with comparable affinities to all of the methylated H3K9, H1K26 and G9aK185 peptides. PMID: 22514736
    19. HIV-1 Vpr displaces heterochromatin protein 1-alpha and heterochromatin protein 1-gamma from chromatin, resulting in premature chromatid separation. PMID: 21875947
    20. we show distinct cell-type- and cancer-stage-associated patterns of key heterochromatin marks: heterochromatic adaptor proteins HP1alpha and HP1gamma, compared with the gammaH2AX marker of endogenously activated DNA damage response. PMID: 20695923
    21. HP1gamma localizes to telomeres in S phase, where it is required to establish/maintain cohesion PMID: 21865325
    22. this study reveals that human NIPBL is a novel protein recruited to DSB sites, and the recruitment is controlled by MDC1, RNF168 and HP1gamma. PMID: 21784059
    23. The Ca2+ channel beta4c subunit interacts with heterochromatin protein 1 gama via a PXVXL binding motif. PMID: 21220418
    24. HP1 gamma binds CD44 pre-mRNA inside the variant region of CD44. PMID: 21358630
    25. Under oxidative conditions, HP1gamma dimerized and held TIF1beta in a chromatin component and inhibited its repression ability. PMID: 20675861
    26. Pax3 represses transcription through a novel mechanism involving competition between corepressor KAP1 and the heterochromatin-binding protein HP1gamma PMID: 16945326
    27. Suv39H1, HP1gamma and histone H3Lys9 trimethylation play a major role in chromatin-mediated repression of integrated HIV-1 gene expression. PMID: 17245432
    28. In non-differentiated cells, transcriptional intermediary factor TIF1beta/heterochromatin protein HP1 interaction occurs only within euchromatin and involves selectively HP1beta and HP1gamma, but not HP1alpha. PMID: 17381543
    29. Overexpression of HP1-GAMMA in wing imaginal discs caused abnormal wings. PMID: 17575412
    30. present an investigation of DNA methylation and histone modification marks across the HNRPA2B1-CBX3 locus in primary peripheral blood mononuclear cells (PBMCs) to characterise the chromatin structure that underlies UCOE activity PMID: 18032920
    31. Heterochromatin protein gamma coordinately regulates CCN2/CTGF transcription by interacting with nuclear MMP3. PMID: 18172013
    32. the spatial proximities among a constellation of functionally related sites that are found within euchromatic regions of the cell nucleus including: HP1gamma, RNA polymerase II, matrin 3, and SAF-A sites PMID: 18618731

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    HGNC: 1553

    OMIM: 604477

    KEGG: hsa:11335

    STRING: 9606.ENSP00000336687

    UniGene: Hs.381189