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  4. Recombinant Human Ceramide kinase (CERK)

Recombinant Human Ceramide kinase (CERK)

  • 货号：
    CSB-YP005256HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005256HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005256HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005256HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005256HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CERK
  • Uniprot No.：
  • 别名：
    Acylsphingosine kinase; Ceramide kinase; Cerk; CERK1_HUMAN; dA59H18.2; dA59H18.3; hCERK; KIAA1646; Lipid kinase 4; Lipid kinase LK4; LK4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-537
  • 氨基酸序列
    MGATGAAEPL QSVLWVKQQR CAVSLEPARA LLRWWRSPGP GAGAPGADAC SVPVSEIIAV EETDVHGKHQ GSGKWQKMEK PYAFTVHCVK RARRHRWKWA QVTFWCPEEQ LCHLWLQTLR EMLEKLTSRP KHLLVFINPF GGKGQGKRIY ERKVAPLFTL ASITTDIIVT EHANQAKETL YEINIDKYDG IVCVGGDGMF SEVLHGLIGR TQRSAGVDQN HPRAVLVPSS LRIGIIPAGS TDCVCYSTVG TSDAETSALH IVVGDSLAMD VSSVHHNSTL LRYSVSLLGY GFYGDIIKDS EKKRWLGLAR YDFSGLKTFL SHHCYEGTVS FLPAQHTVGS PRDRKPCRAG CFVCRQSKQQ LEEEQKKALY GLEAAEDVEE WQVVCGKFLA INATNMSCAC RRSPRGLSPA AHLGDGSSDL ILIRKCSRFN FLRFLIRHTN QQDQFDFTFV EVYRVKKFQF TSKHMEDEDS DLKEGGKKRF GHICSSHPSC CCTVSNSSWN CDGEVLHSPA IEVRVHCQLV RLFARGIEEN PKPDSHS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Shows a greater preference for D-erythro isomer of ceramides. Binds phosphoinositides.
  • 基因功能参考文献：
    1. our findings identify a functional role for Cerk in breast cancer recurrence and suggest the clinical utility of agents targeted against this prosurvival pathway. PMID: 25164007
    2. Data suggest that inhibition of ceramide kinase (CerK) [by specific gene silencing or pharmacological inhibition] drastically reduced cell proliferation in a neuroblastoma cell line. PMID: 22579669
    3. TNF-alpha exposure of human SH-SY5Y neuroblastoma caused a profound increase in CERK activity. PMID: 22230689
    4. Expression of hCERK enhanced ceramide-1-phosphate formation and release of arachidonic acid in Ca(2+) ionophore-stimulated cells. PMID: 21621503
    5. We have identified a differential role for ceramide kinase in mast-cell activation PMID: 21255156
    6. Molecular cloning and functional characterization PMID: 11956206
    7. ceramide kinase and its product, C-1-P, have roles in arachidonic acid release and production of eicosanoids PMID: 12855693
    8. CERK translocates during activation from the cytosol to a lipid raft fraction PMID: 15899891
    9. Pleckstrin homology domain of CERK is not only indispensable for its activity but also act as a regulator of CERK activity. PMID: 16081073
    10. sphingoid chain was also required for substrate recognition by CERK PMID: 16170208
    11. Recombinant CERK was analyzed with regard to dependence on divalent cations, to substrate delivery, specificity, and stereoselectivity. CERK associated with the plasma membrane in CHO cells, which is mediated by the N-terminal putative pleckstrin domain. PMID: 16269826
    12. CERK-pleckstrin homology domain plays a crucial role in plasma membrane targeting through its binding to phosphatidylinositol 4,5-bisphosphate and subsequent induction of ceramide-1-phosphate production in the vicinity of the membrane. PMID: 16488390
    13. These results suggest that ceramide kinase determines the balance between pro-apoptotic ceramide and anti-apoptotic C1P to regulate cell fate, reminiscent of its function in plants. PMID: 17274985
    14. Results demonstrate that CERK localizes to areas of eicosanoid synthesis and uses a ceramide "pool" transported in an active manner via CERT. PMID: 17392267
    15. HL-60 cells are useful for studying CerK functions in leukocyte differentiation, and they also suggest that CerK might have an important role in such differentiation. PMID: 17957442
    16. higher Ca2+ signal observed in hCERK transfected cells as well as the fact that CERK colocalized with EIgG during phagocytosis support hypothesis that Ca2+ signaling is an important factor for increasing phagocytosis and is regulated by CERK PMID: 18156590
    17. identification of a cluster of cysteines, C(347)XXXC(351)XXC(354), essential for CerK function PMID: 18662741
    18. ceramide kinase, was associated with poor pathohistological grading in breast cancer PMID: 19125296
    19. The Pleckstrin Homology (PH) domain of CERK, which is required for Golgi complex localization, can substitute for the N-terminal region of CERKL and allow for wild-type CERKL localization, which is typified by nucleolar accumulation. PMID: 19501188
    20. JNK and ceramide kinase govern the biogenesis of lipid droplets through activation of group IVA phospholipase A2 PMID: 19778898

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  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein.
  • 组织特异性：
    High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung.
  • 数据库链接：

    HGNC: 19256

    OMIM: 610307

    KEGG: hsa:64781

    STRING: 9606.ENSP00000216264

    UniGene: Hs.200668