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  4. Recombinant Human Caspase-6 (CASP6), partial

Recombinant Human Caspase-6 (CASP6), partial

  • 货号：
    CSB-YP004551HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004551HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004551HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004551HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004551HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Apoptotic protease Mch-2; Apoptotic protease MCH2; CASP-6; CASP6; CASP6_HUMAN; Caspase 6; Caspase 6 apoptosis related cysteine protease; Caspase 6; apoptosis related cysteine peptidase; Caspase-6; Caspase-6 subunit p11; Mch2; OTTHUMP00000162742; OTTHUMP00000162743
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 表达区域：
    24-179
  • 氨基酸序列
    AFYKREM FDPAEKYKMD HRRRGIALIF NHERFFWHLT LPERRGTCAD RDNLTRRFSD LGFEVKCFND LKAEELLLKI HEVSTVSHAD ADCFVCVFLS HGEGNHIYAY DAKIEIQTLT GLFKGDKCHS LVGKPKIFII QACRGNQHDV PVIPLDVVD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity. During apoptosis, localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation. Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP. Cleaves phospholipid scramblase proteins XKR4 and XKR9. Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis. Regulates B-cell programs both during early development and after antigen stimulation. In addition, promotes the ZBP1-mediated activation of programmed cell death pathways including pyroptosis, apoptosis, and necroptosis (PANoptosis) and plays an essential role in defense against viruses. Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death.
  • 基因功能参考文献：
    1. The prodomain region was found to be intrinsically disordered independent of the activation state of caspase-6; however, its complete removal resulted in the protection of the adjacent 26-32 region, suggesting that this region may play a regulatory role. The molecular details of caspase-6 dynamics in solution provide a comprehensive scaffold for strategic design of therapeutic approaches for neurodegenerative disorders. PMID: 28864531
    2. SMSr is a novel and specific substrate of caspase-6, a non-conventional effector caspase implicated in Huntington's and Alzheimer's diseases. PMID: 28659495
    3. Results support the possibility that the Casp6 activity in the anterior olfactory nucleus of the olfactory bulb reflects degeneration in the entorhinal cortex and suggest that Casp6 activity in the olfactory bulb could represent degeneration associated with cognitive decline and early Alzheimer disease. PMID: 27931265
    4. These data suggest that caspase-6 deactivating mutations may contribute to multifactorial carcinogenic transformations. PMID: 28726391
    5. Caspase-6 undergoes helix-strand transition upon substrate binding. Caspase-6 shows distinctive conformational dynamics in its 130's region Local pKa Values of Key Amino Acid Residues within the 130's Region Vary between the Unliganded (Helical) and the VEID-bound (Strand) States of Caspase-6 . PMID: 28154009
    6. Following specific binding to and internalization into HER2-overexpressing tumor cells, the e23sFv-Fdt-casp6 protein induced tumor cell apoptosis and inhibited the proliferation of HER2-overexpressing A172 and U251MG cells in vitro, but not in U87MG cells with undetectable HER2 PMID: 27633091
    7. Results identified novel members of the CASP6 interactome and demonstrate that a number of them are involved in key signaling pathways observed in neurodegenerative diseases. PMID: 26908611
    8. The ability of sox11 to reduce effector caspase activity was also reflected in its capacity to reduce cell death following toxic insult. Interestingly, other sox proteins also had the ability to reduce caspase-6 activity but to a lesser extent than sox11 PMID: 26505998
    9. Caspase-6 plays a role in activating caspase-3 in Tau truncation. PMID: 24363090
    10. p53 activity is an important upstream regulator of caspase-6 activity in Huntington's disease. PMID: 24070868
    11. In this study, the crystal structure of a full-length CASP6 zymogen mutant, proCASP6H121A, was solved. PMID: 24419379
    12. Caspase-6 is likely important in most tissues during early development but is less involved in adult tissues PMID: 24265764
    13. Significant associations have been found between CpG sites and patient sex, including DNA methylation in CASP6, a gene that may respond to estradiol treatment, and in HSD17B12, which encodes a sex steroid hormone. PMID: 24058506
    14. Caspase 6 activity in entorhinal cortex identifies aged individuals at risk for developing Alzheimer's disease. PMID: 23402898
    15. Results demonstrate that in the absence of caspase 6 activity, intrinsic triggers of apoptosis induce the receptor-interacting-kinase-1-dependent production of pro-inflammatory cytokines. PMID: 22858542
    16. binding of zinc at the exosite is the primary route of inhibition, potentially locking caspase-6 into the inactive helical conformation. PMID: 22891250
    17. peptide binds at a tetramerization interface that is uniquely present in zymogen caspase-6, rather than binding into the active site, and acts via a new allosteric mechanism that promotes caspase tetramerization PMID: 22683611
    18. Results showed the inhibition mechanism of CASP6 phosphorylation and laid the foundation for a new strategy of rational CASP6 drug design. PMID: 22433863
    19. Caspase-6 cleaves human TERT at residues E129 and D637 as part of the apoptosis pathway in cultured cells. PMID: 21936563
    20. New crystal form of apo-caspase-6 is presented in canonical conformation by identifying the previous apostructure as a hydrogen-ion concentration (pH)-inactivated form of caspase-6. PMID: 21621544
    21. These data suggest that caspase-6 undergoes a significant conformational change upon substrate binding, adopting a structure that is more like canonical caspases. PMID: 21111746
    22. CASP6 can be activated and regulated through intramolecular self-cleavage. PMID: 20890311
    23. Intact IRAK-M is strongly expressed in resting alveolar macrophages but is cleaved in patients with pneumonia via neutrophil-mediated induction of CASP-6 activity. PMID: 21098228
    24. These results imply that pro-Casp6b could negatively regulate pro-Casp6a activation in neurons and prevent Casp6a-mediated axonal degeneration. PMID: 20682790
    25. The NPM mutant specifically inhibits the activities of the cell-death proteases, caspase-6 and -8, through direct interaction with their cleaved, active forms, but not the immature procaspases. PMID: 20606168
    26. results show that p97 is cleaved by Casp6 in Alzheimer's disease and suggest p97 cleavage as an important mechanism for ubiquitin proteasome system impairment PMID: 20427671
    27. p53 activation enhances XIAP inhibition-induced cell death by promoting mitochondrial release of second mitochondria-derived activator of caspases (SMAC) and by inducing the expression of caspase-6. PMID: 19897582
    28. identified executioner caspase-6 as a transcriptional target of p53. The mechanism involves DNA binding by p53 to the third intron of the caspase-6 gene and transactivation. PMID: 12089322
    29. Pro-CASP6 was the only proenzyme whose localization was limited to the cytosol in U937 cells during TPA-induced differentiation. PMID: 12145703
    30. ARK5 negatively regulates procaspase-6 by phosphorylation at Ser257, leading to resistance to the FasL/Fas system. PMID: 15273717
    31. programmed cell death was executed by caspase 6 in Streptococcus pneumoniae infected lung epithelium PMID: 15321985
    32. CASP-6 cleaves the N terminus of tau in vitro at D13, a semicanonical and hitherto undescribed caspase cleavage site in tau. This suggests a role for caspase-6 & N-terminal truncation of tau during neurofibrillary tangle & Alzheimer's disease progression. PMID: 15356202
    33. caspase 6 cleaves periplakin at an unconventional recognition site, amino acid sequence TVAD PMID: 15654952
    34. results suggest that splitting of BL41-E95-A cells induces de novo synthesis of a protein involved in the activation of casp-6 and casp-8, which cleaves 5-LO. PMID: 16135563
    35. data indicate that the CASP6 gene is occasionally mutated in gastric and colorectal carcinomas; also, the data suggest the possibility that deficiency of caspase-6 expression might contribute to the pathogenesis of gastric cancers PMID: 16948818
    36. Caspase-6 was overexpressed in 52.9% of the 210 cases studied, showing predominantly cyoplasmic with some nuclear staining. PMID: 16977583
    37. Active caspase-6 could be an early instigator of neuronal dysfunction. PMID: 17392160
    38. Resveratrol displays converse dose-related effects on fluorouracil-evoked colon cancer cell apoptosis: the role of CASP6 is reported. PMID: 18497562
    39. Expression of caspase 6 and caspase 14 genes were different between normal skin of keloid-prone individuals and normal skin of keloid-resistant patients. PMID: 18762957
    40. Caspase 6 cleaves cyclin B1 during mitotic catastrophe. Mitotic & apoptotic functions may be linked by caspase-dependent processing of mitotic activators. PMID: 18820706
    41. During hypoxia in tube-forming endothelial cells, caspase-7 is responsible for chromatin condensation and nuclear fragmentation while caspase-6 is responsible for DNA ladder formation. PMID: 19022247
    42. The crystal structure of caspase-6, a selective effector of axonal degeneration. PMID: 19694615
    43. Casp-6 is activated in familial forms of Alzheimer disease, as previously observed in sporadic forms. PMID: 19915487

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C14A family
  • 数据库链接：

    HGNC: 1507

    OMIM: 601532

    KEGG: hsa:839

    STRING: 9606.ENSP00000265164

    UniGene: Hs.654616