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  4. Recombinant Human Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A)

Recombinant Human Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A)

  • 货号：
    CSB-YP892176HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP892176HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP892176HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP892176HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP892176HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Calcium/calmodulin dependent protein kinase II alpha; Calcium/calmodulin dependent protein kinase II beta; Calcium/calmodulin dependent protein kinase II delta; Calcium/calmodulin dependent protein kinase II gamma; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II alpha; CaM kinase II; CaM kinase II beta; CaM kinase II delta; CaM kinase II gamma; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2; Camk2a; CAMK2B; CAMK2D; CAMK2G; CAMKA; KCC2A_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-478
  • 氨基酸序列
    MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK GAILTTMLAT RNFSGGKSGG NKKSDGVKES SESTNTTIED EDTKVRKQEI IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE ETRVWHRRDG KWQIVHFHRS GAPSVLPH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission. Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity. Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity.
  • 基因功能参考文献：
    1. SK current is increased via the enhanced activation of CaMKII in patients with atrial fibrillation. PMID: 29737974
    2. Study first demonstrated that CaMKII-alpha was over-expressed in human colon cancers and was associated with cancer differentiation. PMID: 28970726
    3. oxidative stress activates the TRPM2-Ca(2+)-CAMK2 cascade to phosphorylate BECN1 resulting in autophagy inhibition PMID: 27245989
    4. two peptides (SIAPNV(-COOH) and SIVMNV(-COOH)) were identified to have considerably improved affinity with K d increase by ~tenfold relative to wild type peptide. Thus, the two peptides are considered as promising lead entities to develop therapeutic molecular agents with high efficacy and specificity to target CaMKIIalpha-MUPP1 interaction. PMID: 26984442
    5. The importance of CAMK2A and CAMK2B and their auto-phosphorylation in human brain function. PMID: 29100089
    6. This study demonstrate that this ASD-linked de novo CAMK2A mutation disrupts multiple CaMKII functions, induces synaptic deficits, and causes ASD-related behavioral alterations. PMID: 28130356
    7. CaMKII-mediated recruitment and upregulation of CYLD is expected to remove K63-linked polyubiquitins and facilitate proteasomal degradation at the postsynaptic density. PMID: 24614225
    8. CAMK2A SNPs were associated with Alzheimer disease and mild cognitive impairment. AG genotype at the CAMK2A-rs3822606 was associated with AD risk. PMID: 24384746
    9. CaMKII phosphorylates SCN5A in vitro on 23 novel serine sites as was identified by mass spectrometry; reduced S516 phosphorylation has been found in human heart failure. PMID: 25815641
    10. Ca2+/calmodulin-dependent protein kinase-II (CaMKII) has a key role in the plasticity of glutamatergic synapses of the brain. PMID: 25290264
    11. we describe for the first time, two patients with MFD and ID and for whom a deletion encompassing TCOF1 and CAMK2A has been identified PMID: 23695276
    12. a novel regulation of CaMKII by another second messenger system and indicate its involvement in excitotoxic neuronal cell death. PMID: 24855644
    13. Overexpression of a T253D phosphomimic form of calcium/calmodulin-dependent protein kinase type II subunit alpha significantly decreases proliferation, and cells accumulate in mitosis, specifically in metaphase. PMID: 24407174
    14. results suggest that the CAMK2A gene may influence spatial and non-SWM performance in humans without any corresponding gross changes in frontal cortex or hippocampal anatomy. PMID: 22824813
    15. found seven significant associations between CAMK2A SNPs and alcohol dependence, one of which in an autophosphorylation-related area of the gene. PMID: 23459588
    16. These results suggest that Osterix is a novel target of CaMKII and the activity of Osterix can be modulated by a novel mechanism involving CaMKII during osteoblast differentiation. PMID: 23402759
    17. Rem2 has a role in neuronal plasticity hrough co-trafficking with CaMKIIa PMID: 22815963
    18. The decrease in CaMKII signaling in the absence of CAPN3 is associated with a reduction of muscle adaptation response. PMID: 22505582
    19. The role of CaMKII in regulating GLUT4 expression in skeletal muscle. PMID: 22496345
    20. Findigs show that the F-actin-binding protein alpha-actinin-2 targets CaMKIIalpha to F-actin in cells by binding to the CaMKII regulatory domain. PMID: 22427672
    21. Inactivating the alphaCamKII transgene eliminates both the IA-type potassium current-mediated firing decrease and the elevated behavioral response to cocaine. PMID: 22573680
    22. Kv4.3 K channels contribute to cell apoptosis and necrosis through activating CaMKII. PMID: 22023388
    23. Characterization of a central Ca2+/calmodulin-dependent protein kinase IIalpha/beta binding domain in densin that selectively modulates glutamate receptor subunit phosphorylation. PMID: 21610080
    24. 20 single nucleotide polymorphisms had suggestive associations with conduct disorder, nine of which were located in known genes, including CAMK2A PMID: 21611732
    25. Mice heterozygous for a null mutation of the alpha-isoform of calcium/calmodulin-dependent protein kinase II (alpha-CaMKII+/-) have profoundly dysregulated behaviours and impaired neuronal development in the dentate gyrus PMID: 18803808
    26. Knockdown of spinal CaMKIIalpha attentuates opioid-induced hyperalgesia. PMID: 20053885
    27. Four distinct isoforms of CAMKII were isolated. Two of them were characterized as CaMKII alpha and beta subunits.CaMKII expression is developmentally regulated in human fetal and adult brain PMID: 11710563
    28. the NPY Y(1) receptor induces the expression of CRE containing target genes through the CaM kinase-CREB pathway PMID: 11814622
    29. role in cell communication PMID: 11889801
    30. CaMKII alpha mRNA expression is significantly reduced in the prefrontal cortex of patients with bipolar illness. PMID: 11930170
    31. Calcium/calmodulin-dependent protein kinase II binds to Raf-1 and modulates integrin-stimulated ERK activation PMID: 12954639
    32. measured differences in CaMKII binding affinities for CaM play a minor role in the autophosphorylation of the enzyme, largely dictated by autophosphorylation rate for alpha, beta, gamma and delta isoforms PMID: 14722083
    33. CaMKII-alpha may be related with beta-amyloid more closely than being involved in tau hyperphosphorylation in Alzheimer's disease PMID: 15621017
    34. novel mechanism for Ca2+-dependent negative-feedback regulation of NR2B-containing NMDARs in a CaMKII activity- and autophosphorylation-dependent manner that may modulate NMDAR-mediated synaptic plasticity PMID: 15866054
    35. activation of the IKK/NFkappaB signaling cascade by SSTR2 requires a complicated network consisting of Galpha(14), protein kinase C, CamkII, ERK, and c-Src PMID: 16115892
    36. EGF has the ability to abrogate the PP2A function in the maintenance of beta1 integrin-mediated cell adhesion by dissociation of PP2A-IQGAP1-CaMKII from beta1 integrin-Rac through activation of CaMKII PMID: 16557530
    37. CD44 interaction with LARG and EGFR plays a pivotal role in Rho/Ras co-activation, PLC epsilon-Ca2+ signaling, and Raf/ERK up-regulation required for CaMKII-mediated cytoskeleton function and in head and neck squamous cell carcinoma progression PMID: 16565089
    38. To characterize the human alphaCaMKII promoter, a promoter-reporter gene assay using different cell lines, was developed. PMID: 17221287
    39. Skeletal muscle CaMKII kinase isoform expression and serum response factor phosphorylation is higher with endurance-type exercise training, adaptations that are restricted to active muscle. PMID: 17627985
    40. alpha-CaMKII controls the growth of human osteosarcoma by regulating cell cycle progression PMID: 17632540
    41. These findings revealed that TNF-alpha induced VCAM-1 expression via multiple signaling pathways. PMID: 18227124
    42. hCaMKIINalpha suppresses tumor growth by inducing cell cycle arrest via p27 stabilization PMID: 18305109
    43. Phosphorylation or a phosphorylation mimicking mutation on NR2B (NR2B-S1303D) abolishes the Ca(2+)/calmodulin-independent binding whereas it allows the Ca(2+)/calmodulin-dependent binding of alpha-CaMKII in vitro. PMID: 19453375
    44. Data show that data provide a novel mechanism whereby CaMKII may regulate the proteasome in neurons to facilitate remodeling of synaptic connections through protein degradation. PMID: 19638347
    45. Amphetamine sensitization in rats, an animal model of schizophrenia, results in significant increase in CaMKII beta and a nonsignificant increase in CaMKII alpha mRNA. PMID: 17603807
    46. The expression of CaMKIIalpha is significantly elevated in depression (29%), but not in schizophrenia or bipolar disorder, compared to healthy controls. PMID: 16247765
    47. The expression of CaMKII alpha was significantly elevated in depression. PMID: 16247765

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  • 亚细胞定位：
    Cell junction, synapse. Cell junction, synapse, postsynaptic density. Cell projection, dendritic spine. Cell projection, dendrite.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
  • 数据库链接：

    HGNC: 1460

    OMIM: 114078

    KEGG: hsa:815

    STRING: 9606.ENSP00000381412

    UniGene: Hs.743976