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  4. Recombinant Human Calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2)

Recombinant Human Calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2)

  • 货号：
    CSB-YP822299HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP822299HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP822299HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP822299HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP822299HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Calcium/calmodulin dependent protein kinase beta ; Calcium/calmodulin dependent protein kinase kinase 2; Calcium/calmodulin dependent protein kinase kinase 2 beta; Calcium/calmodulin dependent protein kinase kinase beta ; Calcium/calmodulin-dependent protein kinase kinase 2; Calcium/calmodulin-dependent protein kinase kinase beta; CaM kinase kinase beta; CaM KK beta; CaM-kinase kinase 2; CaM-kinase kinase beta; CaM-KK 2; CaM-KK beta; CaMKK 2; CAMKK; CaMKK beta; CAMKK beta protein; Camkk2; CAMKKB; KKCC2_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-588
  • 氨基酸序列
    SSCVSSQPS SNRAAPQDEL GGRGSSSSES QKPCEALRGL SSLSIHLGME SFIVVTECEP GCAVDLGLAR DRPLEADGQE VPLDTSGSQA RPHLSGRKLS LQERSQGGLA AGGSLDMNGR CICPSLPYSP VSSPQSSPRL PRRPTVESHH VSITGMQDCV QLNQYTLKDE IGKGSYGVVK LAYNENDNTY YAMKVLSKKK LIRQAGFPRR PPPRGTRPAP GGCIQPRGPI EQVYQEIAIL KKLDHPNVVK LVEVLDDPNE DHLYMVFELV NQGPVMEVPT LKPLSEDQAR FYFQDLIKGI EYLHYQKIIH RDIKPSNLLV GEDGHIKIAD FGVSNEFKGS DALLSNTVGT PAFMAPESLS ETRKIFSGKA LDVWAMGVTL YCFVFGQCPF MDERIMCLHS KIKSQALEFP DQPDIAEDLK DLITRMLDKN PESRIVVPEI KLHPWVTRHG AEPLPSEDEN CTLVEVTEEE VENSVKHIPS LATVILVKTM IRKRSFGNPF EGSRREERSL SAPGNLLTKK PTRECESLSE LKEARQRRQP PGHRPAPRGG GGSALVRGSP CVESCWAPAP GSPARMHPLR PEEAMEPE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals. Seems to be involved in hippocampal activation of CREB1. May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.
  • 基因功能参考文献：
    1. Silencing of TRPC5 and inhibition of autophagy reverses adriamycin drug resistance in breast carcinoma via CaMKKbeta/AMPKalpha/mTOR pathway. PMID: 28600513
    2. results demonstrated that SSd induces autophagy through the CaMKKbeta-AMPK-mTOR signalling pathway in Autosomal dominant polycystic kidney disease (ADPKD) cells, indicating that SSd might be a potential therapy for ADPKD and that SERCA might be a new target for ADPKD treatment. PMID: 29675630
    3. Three single nucleotide polymorphisms (SNPs) within P2X4R and two SNPs within CAMKK2 influenced concentrations of TNFalpha in peripheral blood mononuclear cells, but these SNP did not associate with risk for HIV-associated sensory neuropathy in South Africans. PMID: 29428485
    4. Serum CAMKK2 was downregulated in female schizophrenic patients compared with female healthy individuals. PMID: 29992499
    5. This study provides insight into functionally disruptive, rare-variant mutations in human CaMKK2, which have the potential to influence risk and burden of disease associated with aberrant CaMKK2 activity in human populations carrying these variants. PMID: 28230171
    6. 14-3-3gamma protein directly interacts with the kinase domain of CaMKK2 and the region containing the inhibitory phosphorylation site Thr(145) within the N-terminal extension. CaMKK isoforms differ in their 14-3-3-mediated regulations and the interaction between 14-3-3 protein and the N-terminal 14-3-3-binding motif of CaMKK2 might be stabilized by small-molecule compounds. PMID: 29649512
    7. CAMKK2 exhibited the strongest associations with HIV-associated sensory neuropathy (HIV-SN), with two SNPs and six haplotypes predicting SN status in black Southern Africans. PMID: 26785644
    8. Study used three cognitive tasks and fMRI to provide convergent evidence of a link between the rs1063843 SNP of CAMKK2 and the function of the dorsolateral prefrontal cortex. In addition, this polymorphism was associated with the function of the striatum during a working memory task. PMID: 27004598
    9. Data suggest that CAMKK2 is highly expressed in high-grade ovarian cancer and ovarian cancer cell lines; CAMKK2 directly activates Akt1 by phosphorylation at Thr-308 in a Ca2+/calmodulin-dependent manner; CAMKK2 knockdown or inhibition decreases Akt1 phosphorylation at Thr-308 and Ser-473. (CAMKK2 = calcium/calmodulin dependent protein kinase kinase 2; AKT1 = AKT serine/threonine kinase 1) PMID: 28634229
    10. Single nucleotide polymorphism in CAMKK2 gene is associated with pulmonary non-tuberculous mycobacterial disease. PMID: 28233049
    11. This study showed that the expression level of CAMKK2 could be regulated by promoter methylation. CAMKK2 serves as a prognostic marker in gliomas and could be a potential therapeutic target in gliomas. PMID: 27012733
    12. For the first time, we showed that rs1063843, a single nucleotide polymorphism located in the CAMKK2 gene, is highly associated with bipolar disorder PMID: 26354101
    13. Site-directed mutagenesis analysis revealed that Leu(358) in CaMKKbeta/Ile(322) in CaMKKalpha confer, at least in part, a distinct recognition of AMPK but not of CaMKIalpha. PMID: 27151216
    14. Clopidogrel diminishes TNFalpha-stimulated VCAM-1 expression at least in part via HO-1 induction and CaMKKbeta/AMPK/Nrf2 pathway in endothelial cells. PMID: 26824050
    15. CaMKK2 (and Nup62) are required for optimal androgen receptor transcriptional activity in castrate resistant prostate cancer cells. PMID: 26552607
    16. Silencing of CAMKK2 using siRNA significantly reduced cell proliferation, colony formation and invasion of gastric cancer cells. PMID: 25756516
    17. CaMKKbeta-AMPKalpha2 signaling contributes to mitotic Golgi fragmentation and the G2/M transition in mammalian cells. PMID: 25590814
    18. CAMKK2 protein is highly up regulated in hepatocellular carcinoma. PMID: 25847065
    19. CaMKK2 plays a pivotal role in the calcium signaling cascade regulating adrenal aldosterone production. PMID: 25679868
    20. PCa patients with miR-224-low/CAMKK2-high expression more frequently had shorter overall survival. PMID: 25394900
    21. evidence supports that CAMKK2 is a novel schizophrenia susceptibility gene. PMID: 23958956
    22. CaMKIIalpha phosphorylation was enhanced by S-Allyl cysteine treatment in a concentration- and time-dependent manner, which paralleled AMPK activation. PMID: 23465592
    23. Pulsatile shear stress mimicking atheroprotective flow increases the level of sirtuin (SIRT)1 in cultured endothelial cells by enhancing its stability, an effect abolished by inhibition or knockdown of CaMKKbeta. PMID: 23754392
    24. CaMKKbeta is involved in AMP-activated protein kinase activation by baicalin in LKB1 deficient cell lines. PMID: 23110126
    25. amino acid starvation regulates autophagy in part through an increase in cellular Ca(2+) that activates a CaMKK-beta-AMPK pathway and inhibits mTORC1, which results in ULK1 stimulation PMID: 23027865
    26. Calcium/calmodulin-dependent protein kinase kinase 2 has roles in signaling and pathophysiology [review] PMID: 22778263
    27. Results suggest that in PCa progression, CaMKK2 and the AR are in a feedback loop in which CaMKK2 is induced by the AR to maintain AR activity, AR-dependent cell cycle control, and continued cell proliferation. PMID: 22654108
    28. Data show that protein kinase A (PKA) regulates the alternative splicing of Ca(2)/calmodulin-dependent protein kinase kinase 2 (CaMKK2) to produce variants that differentially modulate neuronal differentiation. PMID: 21957496
    29. phosphorylation of CaMKKbeta regulates its half-life. PMID: 21669867
    30. Our results demonstrate that CaMKKbeta and AMP-activated protein kinase form a unique signaling complex PMID: 21807092
    31. Findings reveal that hypoxia can trigger AMPK activation in the apparent absence of increased [AMP] through ROS-dependent CRAC channel activation, leading to increases in cytosolic calcium that activate the AMPK upstream kinase CaMKKbeta. PMID: 21670147
    32. These results suggest that CaMKK is an important factor for human cytomegalovirus replication and human cytomegalovirus-mediated glycolytic activation. PMID: 21084482
    33. CaMKK is involved in both S1P receptor- and SR-BI-mediated phosphorylation of AMPK, Akt, and eNOS. PMID: 20018878
    34. ERK activation and cell growth require CaM kinases in MCF-7 breast cancer cells PMID: 19763792
    35. Calmodulin-dependent protein kinase kinase-beta activates AMPK without forming a stable complex. There is a synergistic effects of Ca2+ and AMP. PMID: 19958286
    36. There is a significant basal activity and phosphorylation of AMPK in LKB1-deficient cells that can be stimulated by Ca2+ ionophores, and studies using the CaMKK inhibitor STO-609 and isoform-specific siRNAs show that CaMKKbeta is required for this effect PMID: 16054095
    37. Overexpression of CaMKKbeta in mammalian cells increases AMPK activity, whereas pharmacological inhibition of CaMKK, or downregulation of CaMKKbeta using RNA interference, almost completely abolishes AMPK activation PMID: 16054096
    38. Endothelial cells possess two pathways to activate AMPK, one Ca2+/CaMKKbeta dependent and one AMP/LKB1 dependent. PMID: 16880506
    39. a prominent association found between severity of panic- and agoraphobia symptoms and an exonic SNP (rs3817190) in the CaMKKb gene and a trend for association with an exonic SNP in P2RX7 (rs1718119) with severity scores in the panic- and agoraphobia scale PMID: 17197037
    40. modulating basal AMPK and CAMKKB activity in the hypothalamus is essential for maintaining tight regulation of pathways contributing to food intake PMID: 18436530
    41. Growth of cervical cancer cells was inhibited through activation of CAMKK2 and LBK1. PMID: 19407487
    42. Data show that that the prototypical CaM target sequence skMLCK, a fragment from skeletal muscle myosin light chain kinase, binds to CaM in a highly cooperative way, while only a lower degree of interdomain binding cooperativity emerges for CaMKK. PMID: 19667195

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cell projection, neuron projection.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 组织特异性：
    Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
  • 数据库链接：

    HGNC: 1470

    OMIM: 615002

    KEGG: hsa:10645

    STRING: 9606.ENSP00000312741

    UniGene: Hs.297343