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  4. Recombinant Human CUB domain-containing protein 1 (CDCP1), partial

Recombinant Human CUB domain-containing protein 1 (CDCP1), partial

  • 货号：
    CSB-YP884474HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP884474HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP884474HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP884474HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    9030022E12Rik; AA409659; CD 318; CD_antigen=CD318; CD318; CD318 antigen; CDCP 1; Cdcp1; CDCP1_HUMAN; CUB domain containing protein 1; CUB domain-containing protein 1; E030027H19Rik; FLJ13772; FLJ22375; FLJ22969; Membrane glycoprotein gp140; MGC31813; OTTHUMP00000164120; OTTHUMP00000209568; RGD1305578; SIMA 135; SIMA135; Subtractive immunization M plus HEp3 associated 135 kDa protein; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases; TRASK
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
  • 基因功能参考文献：
    1. Expression of CDCP1 was reduced by AHCC treatment of KLM1-R cells, whereas expression of actin was not affected. The ratio of intensities of CDCP1/actin in AHCC-treated KLM1-R cells was significantly suppressed (p<0.05) compared to untreated cells. PMID: 30396925
    2. Co-expression of CDCP1 and AXL is often observed in EGFR-mutation-positive tumors, limiting the efficacy of EGFR TKIs. Co-treatment with EGFR TKI and TPX-0005 warrants testing. PMID: 29433983
    3. CDCP1 knockdown reduced 3D invasion, which can be rescued by ACSL3 co-knockdown. In vivo, inhibiting CDCP1 activity with an engineered blocking fragment (extracellular portion of cleaved CDCP1) lead to increased LD abundance in primary tumors, decreased metastasis, and increased ACSL activity in two animal models of TNBC. PMID: 28739932
    4. These results revealed that ADAM9 down-regulates miR-1 via activating EGFR signaling pathways, which in turn enhances CDCP1 expression to promote lung cancer progression. PMID: 28537886
    5. CDCP1 is a novel marker of the most aggressive N-positive triple-negative breast cancers; CDCP1 expression and gains in CDCP1 copy number synergized with nodal (N) status in determining disease-free and distant disease-free survival PMID: 27626701
    6. High expression level of CDCP1 is associated with recurrence in glioblastoma. PMID: 26956052
    7. These studies have important implications for the development of a therapeutic to block CDCP1 activity and Triple-negative breast cancer (TNBC)metastasis. PMID: 26876198
    8. CDCP1 may facilitate loss of adhesion by promoting activation of EGFR and Src at sites of cell-cell and cell-substratum contact. PMID: 27495374
    9. Stromal expression patterns for both ADAM12 and CDCP1. PMID: 27685922
    10. these data demonstrated that HIF-2alpha could promote hepatocellular carcinoma cell migration by regulating CDCP1 PMID: 26307391
    11. Our results establish that differential glycosylation, cell surface presentation and extracellular expression of CDCP1 are hallmarks of prostate cancer progression. PMID: 26497208
    12. High CDCP1 expression is associated with Colorectal Cancer. PMID: 25820997
    13. ADAM9 enhances CDCP1 protein expression by suppressing miR-218 for lung tumor metastasis PMID: 26553452
    14. Elevated CDCP1 was observed in 77% of HGSC cases. Silencing of CDCP1 reduced migration and non-adherent cell growth in vitro and tumour burden in vivo. PMID: 26882065
    15. CDCP1 protein plays an important role in the progression of ovarian clear cell carcinoma. Elevated CDCP1 levels correlates with poor patient outcome in ovarian clear cell carcinoma patients. PMID: 25893298
    16. CDCP1 overexpression enhances HER2 activity. CDCP1 binds to HER2, promoting SRC-HER2 crosstalk. PMID: 25892239
    17. Multiple tyrosine phosphorylation sites of CDCP1 are important for the functional regulation of SFKs in several tumor types. PMID: 25728678
    18. These data suggest CDCP1 expression can be used to identify a subset of marrow fibroblasts functionally distinct from CD146+ fibroblasts. PMID: 25275584
    19. The aim of this study was to examine whether activation of Trask may be potentially important in brain metastasis of lung cancers, the commonest site of organ spread and producing the deadliest consequences. PMID: 25775948
    20. decreased CDCP1 expression promoted the invasive and migratory abilities of esophageal cancer cell lines. PMID: 24849519
    21. CDCP1 protein induced by oncogenic Ras/Erk signaling is essential for Ras-mediated metastatic potential of cancer cells. PMID: 24939643
    22. CDCP1 modulates cell-substratum adhesion and motility in colon cancer cell lines. PMID: 25301083
    23. EGF increases the lifespan of CDCP1 promoting its availability on the cell surface where the data indicate it is available to mediate procancer phenotypes such as cell migration. PMID: 24681947
    24. CDCP1 is an essential regulator of the trafficking and function of MT1-MMP- and invadopodia-mediated invasion of cancer cells. PMID: 23439492
    25. CDCP1 represses the epithelial phenotype of pancreatic cancer cells. PMID: 24384474
    26. Complexing of beta1 integrin the 70-kDa with CDCP1 fragment induced intracellular phosphorylation signaling, involving focal adhesion kinase-1 (FAK) and PI3 kinase (PI3K)-dependent Akt activation PMID: 23208492
    27. Expression and phosphorylation of exogenous CDCP1 by Fyn kinase reduced the formation of autophagosomes. PMID: 23510015
    28. In migrating cancer stem cells isolated from primary human colorectal cancers, CD110(+) and CDCP1(+) subpopulations mediate organ-specific lung and liver metastasis. PMID: 23747337
    29. strongly expressed in tumors derived from lung, colon, ovary, or kidney. for a full transformation capacity, the intact amino- and carboxy-termini of CDCP1 are essential. PMID: 23300860
    30. These data support a critical role for CDCP1 as a unique HIF-2alpha target gene involved in the regulation of cancer metastasis. PMID: 23378636
    31. Secreted CDCP1 can be a useful genetic marker for the diagnosis of metastatic prostate cancer. PMID: 22457534
    32. a novel role for CDCP1 in EGF/EGFR-induced cell migration and indicate that targeting of CDCP1 may be a rational approach to inhibit progression of cancers driven by EGFR signaling PMID: 22315226
    33. Data show that the signaling events that accompany the CDCP1 tyrosine phosphorylation observed in cell lines and lung tumors may explain how the CDCP1/SFK complex regulates motility and adhesion. PMID: 21725358
    34. Trask as one of several potential candidates for functionally relevant tumor suppressors on the 3p21.3 region of the genome frequently lost in human cancers. PMID: 21706059
    35. analysis of cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734 PMID: 21994943
    36. CDCP1 is selectively expressed in ovarian tumor vasculature PMID: 21617380
    37. CUB domain-containing protein 1 (CDCP1) is a substrate of Src family kinases and has been shown to regulate anoikis resistance, migration and matrix degradation during tumor invasion and metastasis in a tyrosine phosphorylation-dependent manner. Review. PMID: 21812858
    38. analysis of structural features of Trask that mediate its anti-adhesive functions PMID: 21559459
    39. Src-Trask signaling and Src-focal adhesion signaling inactivate each other, constituting two opposing modes of phosphotyrosine signaling that define a switch underlining cell anchorage state. PMID: 21490433
    40. Data provide molecular mechanisms for the metastasis-enhancing functions of CDCP1. PMID: 21220330
    41. Signal transduction from CDCP1 to PKCdelta leads to its activation, increasing migration of CC-RCC. Furthermore, patient survival can be stratified by CDCP1 expression at the cell surface of the tumor PMID: 21233420
    42. Trask signaling and focal adhesion signaling inactivate each other and signal in exclusion with each other, constituting a switch that underlies cell anchorage state. PMID: 21189288
    43. biological role of this protein and, potentially, its function in cancer, may be mediated by both 70-kDa cell retained and 65-kDa shed fragments, as well as the full-length 135-kDa protein. PMID: 20551327
    44. Overexpression of CDCP1 is associated with pancreatic cancers. PMID: 20501830
    45. In endometrioid adenocarcinoma low CDCP1 and advanced stage were independent poor prognostic factors for both overall and disease-free survival. PMID: 20372833
    46. findings indicate a functional role for CDCP1 in cancer and underscore the therapeutic potential of function-blocking anti-CDCP1 antibodies targeting both primary and metastatic carcinoma cells PMID: 19916495
    47. antibodies generated by subtractive immunization used to purify, identify and partially characterize SIMA135/CDCP1; properties indicate it is a multidomain cell surface antigen, highly expressed by certain cancer cells and normal and cancerous colon PMID: 12660814
    48. tyrosine phosphorylation of CDCP1 is regulated by adhesion or plasmin in epithelial cells PMID: 14739293
    49. CDCP1 is not only a novel marker for immature hematopoietic progenitor cell subsets but also unique in its property to recognize cells with phenotypes reminiscent of MSC and NPC. PMID: 15153610
    50. When CDCP1 promoter was transfected exogenously, Jurkat showed comparable promoter activity with K562, suggesting that the factor to enhance transcription was present but interfered to function in Jurkat PMID: 16926850

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  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Single-pass membrane protein. Note=Shedding may also lead to a soluble peptide.; [Isoform 3]: Secreted.
  • 组织特异性：
    Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well a
  • 数据库链接：

    HGNC: 24357

    OMIM: 611735

    KEGG: hsa:64866

    STRING: 9606.ENSP00000296129

    UniGene: Hs.476093