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  4. Recombinant Human COUP transcription factor 2 (NR2F2)

Recombinant Human COUP transcription factor 2 (NR2F2)

  • 货号：
    CSB-YP016057HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016057HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016057HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016057HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016057HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NR2F2
  • Uniprot No.：
  • 别名：
    Apolipoprotein A-I regulatory protein 1; Apolipoprotein AI regulatory protein 1; ARP-1; ARP1; COT2_HUMAN; COUP TF2; COUP transcription factor 2; COUP transcription factor II; COUP-TF II; COUP-TF2; NR2F2; Nuclear receptor subfamily 2 group F member 2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-414
  • 氨基酸序列
    MAMVVSTWRD PQDEVPGSQG SQASQAPPVP GPPPGAPHTP QTPGQGGPAS TPAQTAAGGQ GGPGGPGSDK QQQQQHIECV VCGDKSSGKH YGQFTCEGCK SFFKRSVRRN LSYTCRANRN CPIDQHHRNQ CQYCRLKKCL KVGMRREAVQ RGRMPPTQPT HGQFALTNGD PLNCHSYLSG YISLLLRAEP YPTSRFGSQC MQPNNIMGIE NICELAARML FSAVEWARNI PFFPDLQITD QVALLRLTWS ELFVLNAAQC SMPLHVAPLL AAAGLHASPM SADRVVAFMD HIRIFQEQVE KLKALHVDSA EYSCLKAIVL FTSDACGLSD VAHVESLQEK SQCALEEYVR SQYPNQPTRF GKLLLRLPSL RTVSSSVIEQ LFFVRLVGKT PIETLIRDML LSGSSFNWPY MAIQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. May be required to establish ovary identity during early gonad development.
  • 基因功能参考文献：
    1. heterozygous frameshift mutations in NR2F2, encoding COUP-TF2, were identified in three children. PMID: 29478779
    2. High COUP-TFII expression is associated with increased lymphangiogenesis and lymph node metastasis in prostate adenocarcinoma. PMID: 29544697
    3. NR2F2 loss-of-function mutation is associated with increased susceptibility to double outlet right ventricle and ventricular septal defect . PMID: 29222010
    4. The results of the present study suggest that COUPTFII functions as a significant regulatory suppressor of gastric cancer growth and metastasis, and suggests that COUPTFII may serve as a novel diagnostic and prognostic biomarker for gastric cancer metastasis. PMID: 29207189
    5. COUP-TFII is a critical factor controlling metastatic gene networks to promote prostate cancer metastasis. PMID: 27108958
    6. Dividing COUP-TFII+ progenitor cells were localized to ventral CGE PMID: 27905023
    7. We showed that the orphan receptor COUP-TFII is an important player in hepatic neoangiogenesis. COUP-TFII expression in hepatic stellate cells (HSC) controls the crosstalk between hepatic HSC and endothelial cells coordinating vascular remodelling during liver injury. PMID: 27866920
    8. Low COUPTFII expression is associated with prostate cancer. PMID: 26895100
    9. We conclude that COUP-TFII mutations can cause diaphragmatic hernias, and should be included in the differential diagnosis of CDH patients, particularly those with comorbid congenital heart defects. PMID: 27363585
    10. Studied the role of Nuclear receptor subfamily 2 group F member 2 (NR2F2) in MSC chondrogenesis in bioprinted cartilage. NR2F2 over-expressed MSCs showed significantly enhanced chondrogenesis while NR2F2 knockdown cells demonstrated the exactly opposite behavior. PMID: 27345768
    11. NR2F2 is a Direct Target Gene of miR-382 in colorectal cancer. PMID: 26800338
    12. Results indicate that COUP-TFII may play an oncogenic role in RCC, and COUP-TFII may promote tumor progression through inhibiting BRCA1. PMID: 27193872
    13. MicroRNA-27b was targeted and down-regulated by NR2F2 in human gastric cancer tissues and cells. PMID: 27844448
    14. NR2F2 could promote TGF-beta-induced epithelial-mesenchymal transition of colorectal carcinoma cells and inhibit Smad7 expression via transactivation of miR-21. PMID: 28192117
    15. NR2F2 mediates BMMSCinduced proliferation of Reh cells, partially via regulation of VEGFA. Disrupting microenvironmental support by targeting NR2F2 may be a potential therapeutic strategy for ALL. PMID: 27314877
    16. miR-382 inhibits prostate cancer cell proliferation and metastasis through inhibiting COUPTFII. PMID: 27748848
    17. Positive COUP-TF II expression levels has significant value in determining CRC stage and metastasis and cooperates with negative Smad4 expression contributing to assess prognosis in patients with colorectal cancer. PMID: 26261604
    18. COUP-TFII (NR2F2) induces down-regulation of cadherin-6 and up-regulation of cadherin-11 in cultured cell lines.high expression of COUP-TFII in some gastric and oesophageal adenocarcinomas, correlated with abnormal cadherin-11 expression. PMID: 25687954
    19. These data demonstrate that Nr2f2 is a direct target of POU4F3 in vitro and that this regulatory relationship may be relevant to hair cell development and survival. PMID: 25372459
    20. TGF-beta inhibited the expression of NR2F2. PMID: 25129343
    21. Overexpression of COUP-TFII was required for cancer cells to metastasise in vivo. PMID: 25032732
    22. These data indicate a novel role for COUP-TFII in suppression of NFkappaB activity. PMID: 24141032
    23. COUP-TFII plays a role in controlling the expression of inflammatory cytokines. PMID: 24176914
    24. Using several human red opsin enhancer/promoter-luciferase reporter constructs, the study found that COUPTFII suppressed intron 3-4 region-dependent luciferase activities. PMID: 24058409
    25. showed that all six coding sequence variants observed in individuals significantly alter the activity of NR2F2 on target promoters PMID: 24702954
    26. In vitro silencing of COUP-TFII reduces the cell growth. PMID: 24122412
    27. Data suggest that NR2F2 and CTNNB1 (catenin beta-1) are aberrantly expressed in uterine fibroid tissue compared with matched myometrium, with strong blood vessel-specific localization. PMID: 23704310
    28. Hey2 and COUP-TFII have an important role in arteriovenous differentiation of human endothelial cells. PMID: 23744056
    29. COUP-TFII orchestrates venous and lymphatic endothelial identity by homo- or hetero-dimerisation with PROX1 PMID: 23345397
    30. Data suggest that NR2F2 is expressed in stromal cells of healthy ovary with little/no expression in epithelial cells; this pattern is markedly disrupted in ovarian cancers with decreased NR2F2 in stroma and ectopic NR2F2 expression in epithelium. PMID: 23690307
    31. Interaction of NSD1 with the NR2E/F subfamily including COUP-TFI, COUP-TFII, EAR2 and TLX requires a F/YSXXLXXL/Y motif. NSD1 interaction with liganded NRs is mediated by an overlapping LXXLL motif. PMID: 23975195
    32. COUP-TFII and related NRs such as the COUPTFs and PNR can selectively associate with the developmental corepressor BCL11A via a conserved motif F/YSXXLXXL/Y within the RID1 and RID2 domains. The interaction with BCL11A facilitates COUP-TFII-mediated repression of the RARb2 gene. PMID: 23975195
    33. MiR-302 inhibits NR2F2 and promotes pluripotency through indirect positive regulation of OCT4. PMID: 23136034
    34. COUP-TFII-V2 negatively regulates the function of COUP-TFII by inhibiting its binding to DNA, decreasing Cyp7a1 expression. PMID: 23458092
    35. In summary, COUP-TFII is expressed in the human fetal forebrain in GABAergic cells, according to its possible role in migration of cortical interneurons. PMID: 22178710
    36. findings reveal that the destruction of the TGF-beta-dependent barrier by COUP-TFII is crucial for the progression of PTEN-mutant prostate cancer into a life-threatening disease PMID: 23201680
    37. COUP-TFII regulates vein identity by suppressing the Notch signal pathway. PMID: 22734039
    38. nucleolin plays a coregulatory role in transcriptional regulation of the tumor suppressor RARB2 by COUP-TFII PMID: 22693611
    39. The C-allele at rs3743462 was associated with increased NR2F2 binding and decreased NR2F2 gene expression. PMID: 22606236
    40. COUP-TFII negatively regulates osteoblast differentiation via interaction with Runx2, and during the differentiation state, BMP2-induced Runx2 represses COUP-TFII expression and promotes osteoblast differentiation. PMID: 22493443
    41. role for the TGFbeta pathway and COUP-TFII in mediating the endothelial transdifferentiating properties of retinoic acid PMID: 20386594
    42. NR2F2 plays a crucial role in the activation of neural genes during early differentiation in humans. PMID: 21151097
    43. Results strongly suggest that NR2F2 is involved in villous CTB cell differentiation and that NR2F2 acts, at least in part, by directly activating TFAP2A gene expression and by potentiating the transactivation of TFAP2A by RARA and RXRA. PMID: 20195529
    44. Data suggest a novel model that the endothelial cell fate regulators, Notch, COUP-TFII, and Prox1, are under an exquisite feedback control mechanism and dynamically regulate each other in LECs. PMID: 20351309
    45. Novel and distinct cell-intrinsic mechanisms mediated by COUP-TF genes to direct the specification and differentiation of progenitor cells; COUP-TFs are crucial for dorsalization of the eye. PMID: 20147377
    46. COUP-TFII represses the alpha-subunit of mitochondrial F1F0 ATP synthase (ATPA) complex and inhibits the USF2-dependent transactivation of the ATPA promoter through competition for an overlapping bindng site in the ATPA cis-acting regulatory element 1. PMID: 12614160
    47. DAX-1 and COUP-TFII may play a role in the modulation of Ad4BP/SF-1-dependent transcription of steroidogenic enzymes in different cell types and follicular stages in normal cycling human ovaries. PMID: 12843196
    48. the DR motif site of the renin gene functions as a negative regulatory element involved in a twofold repression of transcription; nucleic receptors bind the site and are important in renin gene expression; one of the binding proteins may be COUP-TFII. PMID: 17455195
    49. COUP-TFII antagonizes the repression of the PED/PEA-15 gene by HNF-4alpha. PMID: 18765665
    50. Data demonstrate that COUP-TFII is a ligand-regulated nuclear receptor, in which ligands activate the receptor by releasing it from the autorepressed conformation. PMID: 18798693

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  • 相关疾病：
    Congenital heart defects, multiple types, 4 (CHTD4)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR2 subfamily
  • 组织特异性：
    Ubiquitous. Expressed in the stromal cells of developing fetal ovaries.
  • 数据库链接：

    HGNC: 7976

    OMIM: 107773

    KEGG: hsa:7026

    STRING: 9606.ENSP00000377721

    UniGene: Hs.347991