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  4. Recombinant Human Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 (NDST1), partial

Recombinant Human Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 (NDST1), partial

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  • 货号：
    CSB-EP015614HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    NDST1
  • Uniprot No.：
  • 别名：
    NDST1; HSST; HSST1; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; EC 2.8.2.8; Glucosaminyl N-deacetylase/N-sulfotransferase 1; NDST-1; N-heparan sulfate sulfotransferase 1; N-HSST 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1; HSNST 1) [Includes: Heparan sulfate N-deacetylase 1; EC 3.-.-.-); Heparan sulfate N-sulfotransferase 1; EC 2.8.2.-)]
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial of Isoform 2
  • 来源：
    E.coli
  • 分子量：
    73.5kDa
  • 表达区域：
    40-556aa
  • 氨基酸序列
    GWKRGLEPSADAPEPDCGDPPPVAPSRLLPLKPVQAATPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALIIYENILKYVNLDAWNRELLDKYCVAYGVGIIGFFKANENSLLSAQLKGFPLFLHSNLGLKDCSINPKSPLLYVTRPSEVEKGVLPGEDWTVFQSNHSTYEPVLLAKTRSSESIPHLGADAGLHAALHATVVQDLGLHDGIQRVLFGNNLNFWLHKLVFVDAVAFLTGKRLSLPLDRYILVDIDDIFVGKEGTRMKVEDVKALFDTQNELRAHIPNFTFNLGYSGKFFHTGTNAEDAGDDLLLSYVKEFWWFPHMWSHMQPHLFHNQSVLAEQMALNKKFAVEHGIPTDMGYAVAPHHSGVYPVHVQLYEAWKQVWSIRVTSTEEYPHLKPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSSELDKIINGGELFLTVLLNPVSAPQPMAAGEKGLLHSLSAADTGFLEPGKGGEA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response. Required for the exosomal release of SDCBP, CD63 and syndecan.
  • 基因功能参考文献：
    1. Compound heterozygous mutations in NDST1 were identified, in the heparan sulfate N deacetylatase domain of one allele and the sulfotransferase domain of the other allele. This report expands the phenotypic spectrum of Ndst1 deficiency in humans. PMID: 28211985
    2. Among the genes enriched in this screening, the s found that TM9SF2 is critical for N-sulfation of heparan sulfate and therefore for chikungunya virus infection because it is involved in the proper localization and stability of NDST1. PMID: 28404855
    3. Our data confirm NDST1 mutations as a cause of autosomal recessive intellectual disability with a distinctive phenotype, and support an important function of NDST1 in human development. PMID: 25125150
    4. demonstrate the essential role of domain cooperation within NDST-1 in producing HS with specific domain structures PMID: 26109066
    5. Upregulation of NDST1 is associated with chemoresistance in breast cancer. PMID: 25156775
    6. These findings establish NDST1 as a target of miR-24 and demonstrate how such NDST1 suppression in endothelial cells results in reduced responsiveness to VEGFA PMID: 23884416
    7. MicroRNA-191 targets N-deacetylase/N-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803 PMID: 21947487
    8. Inhibitory peptides of the sulfotransferase domain of the heparan sulfate enzyme, N-deacetylase-N-sulfotransferase-1. PMID: 20129923
    9. Altered expression of NDST-1 mRNA in glomerular basement membrane in puromycin aminonucleoside nephrosis. PMID: 14966466
    10. effect of targeted inactivation of the Ndst1 gene on the inflammatory response associated with allergic inflammation PMID: 19710461
    11. the expression of the NDST-1 isoform was approximately equal at all stages of mast cell maturation PMID: 19915053

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  • 相关疾病：
    Mental retardation, autosomal recessive 46 (MRT46)
  • 亚细胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Sulfotransferase 1 family, NDST subfamily
  • 组织特异性：
    Widely expressed. Expression is most abundant in heart, liver and pancreas.
  • 数据库链接：

    HGNC: 7680

    OMIM: 600853

    KEGG: hsa:3340

    STRING: 9606.ENSP00000261797

    UniGene: Hs.222055