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  4. Recombinant Human Barrier-to-autointegration factor (BANF1)

Recombinant Human Barrier-to-autointegration factor (BANF1)

  • 货号：
    CSB-YP002550HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002550HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002550HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002550HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002550HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BANF1
  • Uniprot No.：
  • 别名：
    BAF; BAF_HUMAN; BANF 1; BANF1; Barrier to autointegration factor 1; Barrier to autointegration factor; Barrier-to-autointegration factor; BCRG 1; BCRG1; BCRP 1; BCRP1; Breakpoint cluster region protein 1; D14S1460; MGC111161; NGPS
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-89
  • 氨基酸序列
    MTTSQKHRDF VAEPMGEKPV GSLAGIGEVL GKKLEERGFD KAYVVLGQFL VLKKDEDLFR EWLKDTCGAN AKQSRDCFGC LREWCDAFL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging.; (Microbial infection) Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.; (Microbial infection) In case of poxvirus infection, has an antiviral activity by blocking viral DNA replication.
  • 基因功能参考文献：
    1. BAF and BAF-L might contribute to the shaping of the spermatozoon nucleus and, after fertilisation, its transition to the male pronucleus. PMID: 28684548
    2. we demonstrate that BAF is necessary to modulate prelamin A effects on chromatin structure PMID: 26701887
    3. Findings support the value of using BioID to identify unrecognized constituents of distinct subcellular compartments refractory to biochemical isolation and reveal VRK2A as a transmembrane kinase in the NE that regulates BAF. PMID: 28637768
    4. It has been concluded that the LEM domain, responsible for binding to the chromatin protein BAF, undergoes a conformational change during self-assembly of emerin N-terminal region. PMID: 27960036
    5. antiviral capabilities of the barrier to autointegration factor (BAF/BANF1) and how its function and regulation places BAF at the junction of multiple pathways protecting a cell's genetic integrity PMID: 26842478
    6. The findings unveil a unique mechanism where the nuclear periphery proteins lamin-A/C, LAP2alpha and BAF1 are assembled into a protein complex during mitosis in order to regulate assembly and positioning of the mitotic spindle. PMID: 26092935
    7. results demonstrate a novel function of BAF as an epigenetic regulator of HSV lytic infection; hypothesize that BAF facilitates Herpes Simplex Virus IE and E gene expression by recruiting the SETD1A methyltransferase to viral IE and E gene promoters PMID: 26015494
    8. Vaccinia virus B1 kinase is needed for multiple critical junctures in the poxviral life cycle in a manner that is both dependent on and independent of BAF. PMID: 26223647
    9. BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy. PMID: 25991860
    10. These data suggest that VRK3-mediated phosphorylation of BAF may facilitate DNA replication or gene expression by facilitating the dissociation of nuclear envelope proteins and chromatin during interphase. PMID: 25899223
    11. Association of emerin with nuclear BAF in cells required the LEM domain (residues 1-47). PMID: 25052089
    12. The BANF1, alanine 12 threonine (A12T) mutant is impaired in its ability to bind DNA while its interaction with nuclear envelope proteins is unperturbed. PMID: 25495845
    13. Altogether, these data demonstrate that phosphoregulation of BAF by viral and cellular enzymes modulates this protein at multiple molecular levels, thus determining its effectiveness as an antiviral factor and likely other functions as well. PMID: 24600006
    14. Data indicate that the major phosphatase responsible for dephosphorylation of of BAF Ser-4 to be protein phosphatase 4 catalytic subunit. PMID: 24265311
    15. Activation of BANF1 possibly suppresses S100A9 expression and inactivates c-Jun, resulting in suppression of cutaneous inflammation PMID: 23664529
    16. Emerin and BAF associated only in histone- and lamin-B-containing fractions. The S173D mutation specifically and selectively reduced GFP-emerin association with BAF by 58% PMID: 24014020
    17. The decrease in vaccinia virus transcription caused by loss of B1 kinase can be rescued by depletion of BAF. PMID: 23891157
    18. The accumulation of wild-type prelamin A detected in restrictive dermopathy as well as the accumulation of mutated forms identified in familial partial lipodystrophy and mandibuloacral dysplasia affect the nuclear localization of BAF protein. PMID: 22935701
    19. BAF associated in vivo with SET/I2PP2A (protein phosphatase 2A inhibitor; blocks H3 dephosphorylation) and G9a (H3-K9 methyltransferase), but showed no detectable association with HDAC1 or HATs. PMID: 22127260
    20. A single copy of normal BANF1 is sufficient to avoid the development of Nestor-Guillermo progeria syndrome. PMID: 21932319
    21. The absence of direct binding of BAF to MAN1-C eliminates disruption of this interaction as the cause of the premature aging phenotype. PMID: 21966431
    22. Data determined that the knockdown of Banf1 alters the cell cycle distribution of both human and mouse ESCs by causing an uncharacteristic increase in the proportion of cells in the G2-M phase of the cell cycle. PMID: 21750191
    23. The s demonstrate that the DNA binding and dimerization capabilities of BAF are essential for its function as an antipoxviral effector, while the presence of emerin is not required. PMID: 21880762
    24. These nuclear abnormalities are rescued by ectopic expression of wild-type BANF1, providing evidence for the causal role of this mutation. PMID: 21549337
    25. Cooperation of ATP-dependent remodeling, histone methylation, and kinase activation, followed by H1 displacement, is a prerequisite for the subsequent displacement of histone H2A/H2B catalyzed by PCAF and BAF. PMID: 21447625
    26. These findings are supported by coimmunoprecipitation of prelamin A or progerin with BAF in vivo and suggest that BAF could mediate prelamin A-induced chromatin effects. PMID: 20581439
    27. BAF and emerin have dynamic roles in genome integrity and might help couple DNA damage responses to the nuclear lamina network PMID: 19759913
    28. The barrier-to-autointegration factor (BAF)-binding domain of emerin is located at the emerin N-terminus (residues 70-178) and includes the LEM-domain. PMID: 11792821
    29. BAF is required for the assembly of emerin and A-type lamins at the reforming nuclear envelope during telophase and may mediate their stability in the subsequent interphase. PMID: 11792822
    30. BAF may have a role in regulating emerin-GCL repressor complexes PMID: 12493765
    31. report that BAF protein is a component of preintegration complexes isolated from HIV-1-infected cells PMID: 12663813
    32. HB and barrier-to-autointegration factor are the same protein PMID: 14523012
    33. BAF protein was detected in activated but not resting CD4+ T-lymphocytes. BAF bound directly to both p55 Gag and its cleaved product, matrix. PMID: 14645565
    34. Data describe the mobility of barrier-to-autointegration factor to its partners emerin, LAP2 beta, and MAN1 in the nuclear membrane of living HeLa cells. PMID: 15109603
    35. LAP2alpha and BAF transiently localize to telomeres and specific regions on chromatin during nuclear assembly PMID: 15546916
    36. BAF bridges DNA using two pairs of helix-hairpin-helix motifs located on opposite surfaces of the BAF dimer without changing its conformation. PMID: 16155580
    37. phosphorylation at Ser175 regulates the dissociation of emerin from BAF PMID: 16204256
    38. Ser-4 phosphorylation inhibits BAF binding to emerin and lamin A, and thereby weakens emerin-lamin interactions during both mitosis and interphase. PMID: 16371512
    39. These results indicate that barrier-to-autointegration factor is required for the integrity of the nuclear lamina and normal progression of S phase in human cells. PMID: 17519288
    40. We now show that BAF acts as a potent inhibitor of poxvirus replication unless its DNA-binding activity is blocked by B1-mediated phosphorylation. PMID: 18005698
    41. analyses in human cells showed that barrier-to-autointegration factor (BAF), assembled first at the distinct ;core' region of the telophase chromosome and formed an immobile complex by binding with NE proteins, such as lamin A and emerin. PMID: 18628300

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  • 相关疾病：
    Nestor-Guillermo progeria syndrome (NGPS)
  • 亚细胞定位：
    [Barrier-to-autointegration factor]: Nucleus. Cytoplasm. Chromosome. Nucleus envelope.
  • 蛋白家族：
    BAF family
  • 组织特异性：
    Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.
  • 数据库链接：

    HGNC: 17397

    OMIM: 603811

    KEGG: hsa:8815

    STRING: 9606.ENSP00000310275

    UniGene: Hs.433759