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  4. Recombinant Human Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (MGAT5), partial

Recombinant Human Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (MGAT5), partial

  • 货号：
    CSB-YP600992HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP600992HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP600992HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP600992HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP600992HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MGAT5
  • Uniprot No.：
  • 别名：
    6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; 6-N-acetylglucosaminyltransferase; Alpha 1,3(6) mannosylglycoprotein; Alpha 1,6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase; Alpha mannoside beta 1,6 N acetylglucosaminyltransferase; Alpha-1; Alpha-mannoside beta-1; Beta 1,6 N acetyl glucosaminyltransferase; GGNT5; GlcNAc T V; GlcNAc-T V; GNT V; GNT VA; GNT-V; GNTV; GNTVA; Mannoside acetylglucosaminyltransferase 5; Mannosyl (alpha 1,6) glycoprotein beta 1,6 N acetyl glucosaminyltransferase ; Mgat5; MGT5A_HUMAN; N acetylglucosaminyl transferase V; N acetylglucosaminyltransferase V mannosyl (alpha 1,6) glycoprotein; N-acetylglucosaminyl-transferase V
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. Catalyzes an important step in the biosynthesis of branched, complex-type N-glycans, such as those found on EGFR, TGFR (TGF-beta receptor) and CDH2. Via its role in the biosynthesis of complex N-glycans, plays an important role in the activation of cellular signaling pathways, reorganization of the actin cytoskeleton, cell-cell adhesion and cell migration. MGAT5-dependent EGFR N-glycosylation enhances the interaction between EGFR and LGALS3 and thereby prevents rapid EGFR endocytosis and prolongs EGFR signaling. Required for efficient interaction between TGFB1 and its receptor. Enhances activation of intracellular signaling pathways by several types of growth factors, including FGF2, PDGF, IGF, TGFB1 and EGF. MGAT5-dependent CDH2 N-glycosylation inhibits CDH2-mediated homotypic cell-cell adhesion and contributes to the regulation of downstream signaling pathways. Promotes cell migration. Contributes to the regulation of the inflammatory response. MGAT5-dependent TCR N-glycosylation enhances the interaction between TCR and LGALS3, limits agonist-induced TCR clustering, and thereby dampens TCR-mediated responses to antigens. Required for normal leukocyte evasation and accumulation at sites of inflammation. Inhibits attachment of monocytes to the vascular endothelium and subsequent monocyte diapedesis.; Promotes proliferation of umbilical vein endothelial cells and angiogenesis, at least in part by promoting the release of the growth factor FGF2 from the extracellular matrix.
  • 基因功能参考文献：
    1. The best homology model is consistent with available experimental data. The three-dimensional model, the structure of the enzyme catalytic site and binding information obtained for the donor and acceptor can be useful in studies of the catalytic mechanism and design of inhibitors of GnT-V. PMID: 26821880
    2. Our data suggest that oxidative stress induces the overexpression of MGAT5 via the regulation of the focal adhesion kinase-extracellular signal-regulated kinase signaling pathway, which, in turn, affects the function of endothelial cells, which then participates in the pathogenesis of preeclampsia. PMID: 27334383
    3. PTPalpha is identified as a novel substrate of N-Acetylglucosaminyltransferase V (GnT-V) and could be a factor regulating promotion of migration in breast cancer cells PMID: 27965091
    4. Tunicamycin, an inhibitor of N-glycan biosynthesis, was also able to enhance the radiosensitivity of U251 cells. Thus, our results suggest that development of therapeutic approaches targeting N-linked beta1,6-GlcNAc branches which are encoded by N-acetylglucosaminyltransferase V may be a promising strategy in glioblastoma treatment PMID: 26526581
    5. the knockdown of GnTV significantly suppressed the proliferation, migration and invasion (P<0.05) of the SMMC7721/R cells. PMID: 26531171
    6. role in the inhibition of trophoblast cell invasion and migration during early pregnancy by direct or indirect regulation of MMP2/9 activity PMID: 26349781
    7. the level of TGFBR1 and early osteogenic differentiation were abolished in the DPSCs transfected with siRNA for GnT-V knockdown...GnT-V plays a critical role in the hexosamine-induced activation of TGF-b signaling and osteogenic differentiation PMID: 26583147
    8. Mgat5 plays an important role in early spontaneous miscarriage in humans. PMID: 26109616
    9. Gnt-V caused tumour growth more quickly PMID: 26293457
    10. High expression of GnT-V was observed in infiltrating cells in skin section samples from systemic and localized patients with scleroderma. PMID: 25876794
    11. MGAT5 protein and gene expression in in uveal and cutaneous melanoma cells PMID: 26098720
    12. Human Mgat5 increases amino acid uptake, intracellular levels of glycolytic and TCA intermediates, as well as HEK293 cell growth. PMID: 25395405
    13. Study disclose that a deficiency in branched N-glycosylation on TCR due to a reduced MGAT5 gene expression is a new molecular mechanism underlying UC pathogenesis. PMID: 24334766
    14. The results identified Mgat5-mediated beta-1-6-GlcNAc branched N-glycosylation and following activation of EGFR as a potential novel upstream molecular event for PAK1-induced anoikis resistance in hepatoma cells. PMID: 23811795
    15. These results contribute to new insight into the underlying molecular mechanisms of GnT-V regulation in gastric cancer with potential translational clinical applications. PMID: 24399258
    16. GnT-V plays a significant role in metastasis and invasion in gastric cancer cells. PMID: 23563846
    17. The combination of intratumoral MGAT5 expression and TNM or Kiel staging systems had a better predictive power for overall survival. PMID: 23107376
    18. GnT-V directs cancer progression by modulating MMPs in cancer. PMID: 23357422
    19. The results of this study have shown that the MGAT5 intronic variants rs4953911 and rs3814022 correlate with lower N-glycan branching, reduced surface CTLA-4 in human CD4 + T cell blasts, and associate with multiple sclerosis. PMID: 23351704
    20. Data show that knockdown of CD147 inhibited MMP-2 activity of GnT-V-overexpressing cells, indicating that aberrant beta1,6-branches on CD147 is crucial for the induction of MMPs (matrix metalloproteinases) in SMMC-7721 cells. PMID: 23005037
    21. The results suggest that GnT-V may be a potential target for predicting nasopharyngeal carcinoma response to radiotherapy. PMID: 22780953
    22. These data suggested that GnT-V expression was positively related with malignancy in human hepatocellular carcinoma (HCC) and GnT-V may be both a differentiation marker and a potential target for the treatment of HCC. PMID: 22537550
    23. 33 directly measured and 13 derived glycosylation traits in 3533 individuals were identified and three novel gene association (MGAT5, B3GAT1 and SLC9A9) were identified using an additional European cohort. PMID: 21908519
    24. Inhibiting expression of N-acetylglucosaminyltransferase V inhibits the proliferation of PC-3 cells. PMID: 20584650
    25. GnT-V expression is positively correlated with malignancy in nasopharyngeal carcinoma cells PMID: 21676538
    26. Findings suggest Mgat5 may play an important role during oncogenesis, identifying a potential therapeutic target for pulmonary adenocarcinoma. PMID: 21631992
    27. The rs1257169(G) allele of MGAT5 is associated with lower disease severity in multiple sclerosis PMID: 21115203
    28. The GnT-V was fully active without exogenous cation and in the presence of EDTA, and pH optimum for GnT-V was in the range of 6.5-7.0. PMID: 19846580
    29. Our results suggest that GnT-V could decrease human hepatoma SMMC-7721 cell adhesion and promote cell proliferation partially through RPTPkappa. PMID: 19911372
    30. Prometastatic effect of N-acetylglucosaminyltransferase V is due to modification and stabilization of active matriptase by adding beta 1-6 GlcNAc branching PMID: 11864986
    31. A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V distinct from the original glycosyltransferase activity. PMID: 11872751
    32. Low GnT-V expression is associated with shorter survival and poor prognosis in pStage I overall Non-small cell lung cancer. PMID: 15014031
    33. Data describe the effect of N-acetylglucosaminyltransferase V on the expressions of other glycosyltransferases involved in the synthesis of surface sialyl Lewis X antigen. PMID: 15044007
    34. GlcNAc-transferase-V activity is up-regulated in RA and Vit-D(3)-treated hepatoma cell lines. PMID: 15313475
    35. GnT-V would contribute to placentation in the early phase of pregnancy, possibly regulating the process of invasion of trophoblast cells PMID: 15809094
    36. These results supported the mechanism that blocking of GnT-V expression impaired functions of chaperones and N-glycan-synthesizing enzymes, which caused UPR in vivo. PMID: 16467879
    37. GnT-V is closely related to low malignant potential and good prognosis of the patients with bladder cancer. PMID: 16638859
    38. Glycosylation caused by GnT-V directs integrin beta1 stability and more delivery to plasma membrane, subsequently promotes Fn-based cell migration and invasion. PMID: 16924681
    39. glycosylation change & decrease of transport activity of GLUT1 may be 1 possible mechanism of ER stress induced by down-regulating GnT-V, & GnT-V may contribute to the regulation of glucose uptake by modifying glycosylation of GLUT1 in some tumor cells. PMID: 17451637
    40. We investigated mRNA levels of glycosyltransferases, namely GnT-V, and found that it's expression was decreased in HLE-cells resistant to Epirubicin as well as in HLE-cells resistant to Mitoxantrone. PMID: 17488527
    41. Tissue inhibitor of metalloproteinase-1 (TIMP-1), was identified as a target protein for GnT-V in human colon cancer cell WiDr. PMID: 17878270
    42. Results suggested that high GnT-V expression was correlated with an unfavourable clinical outcome. PMID: 17971775
    43. Knock-down of MGAT5 in PC-3 cells attenuated the metastatic ability of prostate cancer cells, as determined by the in vitro invasion assay and the xenograft animal studies. PMID: 18649738
    44. GnT-V expression is correlated with a poor prognosis in gastric cancer patients due to metastases. PMID: 18931531
    45. decreased GnT-Va activity due to siRNA expression in human carcinoma cells inhibits ligand-induced EGFR internalization, consequently resulting in delayed downstream signal transduction and inhibition of the EGF-induced, invasiveness-related phenotypes. PMID: 19225046
    46. overexpression of GnT-V in a hepatoma cell line not only induced the addition of beta1,6 GlcNAc branch to N-glycan of RPTPkappa but also decreased the protein level of RPTPkappa PMID: 19236842
    47. High expression of N-acetylglucosaminyltransferase V is associated with mucinous tumors of the ovary. PMID: 19787216

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  • 亚细胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein.; [Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A]: Secreted.
  • 蛋白家族：
    Glycosyltransferase 18 family
  • 数据库链接：

    HGNC: 7049

    OMIM: 601774

    KEGG: hsa:4249

    STRING: 9606.ENSP00000281923

    UniGene: Hs.4988