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  4. Recombinant Human Abl interactor 1 (ABI1)

Recombinant Human Abl interactor 1 (ABI1)

  • 货号：
    CSB-YP812910HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP812910HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP812910HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP812910HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP812910HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ABI1
  • Uniprot No.：
  • 别名：
    Abelson interactor 1; Abi-1; Abi1; ABI1_HUMAN; Abl binding protein 4; Abl interactor 1; Abl interactor protein 1; Abl-binding protein 4; AblBP4; e3B1; Eps8 binding protein; Eps8 SH3 domain binding protein; Eps8 SH3 domain-binding protein; Eps8-binding protein; Hssh3bp1; Interactor protein AblBP4; NAP1; Nap1 binding protein; Nap1-binding protein; Nap1BP; Spectrin SH3 domain binding protein 1; Spectrin SH3 domain-binding protein 1; SSH3BP
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-508
  • 氨基酸序列
    AELQMLLEE EIPSGKRALI ESYQNLTRVA DYCENNYIQA TDKRKALEET KAYTTQSLAS VAYQINALAN NVLQLLDIQA SQLRRMESSI NHISQTVDIH KEKVARREIG ILTTNKNTSR THKIIAPANM ERPVRYIRKP IDYTVLDDVG HGVKWLKAKH GNNQPARTGT LSRTNPPTQK PPSPPMSGRG TLGRNTPYKT LEPVKPPTVP NDYMTSPARL GSQHSPGRTA SLNQRPRTHS GSSGGSGSRE NSGSSSIGIP IAVPTPSPPT IGPENISVPP PSGAPPAPPL APLLPVSTVI AAPGSAPGSQ YGTMTRQISR HNSTTSSTSS GGYRRTPSVT AQFSAQPHVN GGPLYSQNSI SIAPPPPPMP QLTPQIPLTG FVARVQENIA DSPTPPPPPP PDDIPMFDDS PPPPPPPPVD YEDEEAAVVQ YNDPYADGDP AWAPKNYIEK VVAIYDYTKD KDDELSFMEG AIIYVIKKND DGWYEGVCNR VTGLFPGNYV ESIMHYTD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.
  • 基因功能参考文献：
    1. our findings indicated that ABI1 contributes to the development and progression of hepatocellular carcinoma (HCC) as an oncogene and may serve as a valuable prognostic marker for HCC patients. PMID: 28339046
    2. these data indicate that HCV exploits host Abi1 protein via NS5A to modulate MEK/ERK signaling pathway for its own propagation. PMID: 27551040
    3. Suggest that Abi1 acts as a tumor-promoting gene in epithelial ovarian cancer progression, which may lead to unfavorable prognosis. PMID: 26193797
    4. These results indicate that the alpha4-Abi-1 signaling pathway may mediate acquisition of the drug-resistant phenotype of leukemic cells. PMID: 24699303
    5. Our data indicate that phosphorylated Abi1 contributes to the invasive properties of colorectal cancer. PMID: 24913355
    6. Abi1 is expressed at the invasive front of colorectal carcinomas and localizes to the leading edge of lamellipodia in cultured colorectal carcinoma cells. PMID: 24196611
    7. Data indicate that Abi1 is activated by the c-Abl-Crk-associated substrate (CAS) pathway, and Abi1 reciprocally controls the activation of its upstream regulator c-Abl. PMID: 23740246
    8. a possible role for Abi1 as a marker for early KRAS mutation in hyperplastic polyps PMID: 22808230
    9. Breast tumors expressing high levels of Abi1 are significantly associated with early recurrence and worse survival on multivariate analysis. PMID: 21046228
    10. Down-regulation of E3B1/ABI-1 expression in human carcinomas may play a critical role in tumor progression and in determining disease prognosis. PMID: 22430194
    11. The phosphorylation and dephosphorylation cycle of VASP by the Abi-1-bridged mechanism regulates association of VASP with focal adhesions, which may regulate adhesion of Bcr-Abl-transformed leukaemic cells. PMID: 22014333
    12. CDK1-mediated phosphorylation of Abi1 attenuates Bcr-Abl-induced F-actin assembly and tyrosine phosphorylation of WAVE complex during mitosis PMID: 21900237
    13. Expression of Abi1 is up-regulated in hepatocellular carcinoma tissues compared with corresponding para carcinomatous liver tissue, and is significantly correlated with tumor number, capsular formation, venous invasion, grade and prognosis. PMID: 20137729
    14. Data show that disruption of phosphorylation sits Y398 and Y213 significantly weakens the binding of Abi-1 to c-Abl. PMID: 21320496
    15. Study implicates that the integrity of SOS1/EPS8/ABI1 tri-complex is a determinant of ovarian cancer metastasis. PMID: 21118970
    16. Downregulation of ABI1 expression in human gastric carcinoma may play a critical role in tumor progression and in determining patient prognosis. PMID: 19554484
    17. Macropinocytosis is regulated by interactions between Abi1 pY213 and the C-terminal SH2 domain of p85-thereby linking Abl kinase signaling to p85-dependent regulation of macropinocytosis. PMID: 20598684
    18. Abi1 isoforms differentially regulate macropinocytosis as a consequence of their different relative affinities for activated Rac1 in Wave 2 complex. PMID: 20479892
    19. The ABI1 gene is down-regulated in gastric cancer cells. ABI1 overexpression effectively inhibits cell proliferation. PMID: 20193272
    20. Combination of experimental and computational interactome research was used for the analysis of protein-protein interactions between Abi-1 and VASP in human platelets. PMID: 20110575
    21. t(10;11)(p11.2;q23) involving MLL and this gene is associated with congenital acute monocytic leukemia PMID: 12547160
    22. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins PMID: 12672821
    23. Abi1 interacts directly with the WHD domain of WAVE2, increases WAVE2 actin polymerization activity and mediates the assembly of a WAVE2-Abi1-Nap1-PIR121 complex. PMID: 15048123
    24. Abi enhances Abl-mediated downregulation and phosphorylation of Cdc2 kinase PMID: 15591787
    25. Abi-1-mediated coupling of Abl to WAVE2 promotes Abl-evoked WAVE2 tyrosine phosphorylation required to link WAVE2 with activated Rac and with actin polymerization and remodeling at the cell periphery. PMID: 15657136
    26. We demonstrate a cell-type-dependent requirement for various WASP-related proteins in Listeria entry and InlB-induced membrane ruffling; Abi1, a key component of WAVE complexes, is recruited at the entry site PMID: 15769844
    27. Abi-1 promotes Abl-mediated BCAP phosphorylation and suggest that Abi-1 in general coordinates kinase-substrate interactions PMID: 15893754
    28. Overexpression of e3B1 in NIH3T3/EGFR cells sensitized EGF-induced activation of Rac1, whereas it had no impact on EGF-induced activation of p21Ras. PMID: 16182283
    29. results suggest that the Eps8/Abi1 complex is capable of regulating the localization and/or activity of actin nucleators PMID: 16225669
    30. c-Abl activates WAVE2 via tyrosine phosphorylation to promote actin remodeling in vivo; Abi-1 forms the crucial link between these two factors PMID: 16899465
    31. NESH (Abi-3), like Abi-1 and Abi-2, is a component of the Abi/WAVE complex, but likely plays a different role in the regulation of c-Abl. PMID: 17101133
    32. Data show that Chlamydia trachomatis activates Rac and promotes its interaction with WAVE2 and Abi-1 to activate the Arp2/3 complex resulting in the induction of actin cytoskeletal rearrangements that are required for invasion. PMID: 17501982
    33. by activating the Abi1 pathway, Bcr-Abl induces a translocation of MT1-MMP to a membrane-associated structural complex enriched with F-actin and adhesion molecules. PMID: 17943163
    34. ABI-1 plays an important role in the spread of breast cancer and this role may be mediated via the phosphatidylinositol 3-kinase pathway. PMID: 17951403
    35. c-Abl kinase inhibition mediated by a phosphotyrosine located in trans in the c-Abl substrate, Abi1. PMID: 18328268
    36. These findings uncover a novel link between cadherin-mediated adhesion and the regulation of actin dynamics through the requirement for an Abi/Dia complex for the formation and stability of cell-cell junctions. PMID: 19158278
    37. Abl interactor 1 regulates Src-Id1-matrix metalloproteinase 9 axis and is required for invadopodia formation, extracellular matrix degradation and tumor growth of human breast cancer cells PMID: 19843640
    38. Human SSH3BP1 is a marker of macropinocytic vesicles and potential regulator of macropinocytosis in mammalian cells. SSH3BP1 may play a role in fusion of macropinocytic vesicles. PMID: 11034908

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  • 相关疾病：
    A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with KMT2A/MLL1. ABI1 isoform 2 was found to be present in acute leukemia KMT2A/MLL1-ABI1 fusion transcript.
  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, growth cone. Cell junction, synapse, postsynaptic density. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    ABI family
  • 组织特异性：
    Widely expressed, with highest expression in brain.
  • 数据库链接：

    HGNC: 11320

    OMIM: 603050

    KEGG: hsa:10006

    STRING: 9606.ENSP00000365312

    UniGene: Hs.508148