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  4. Recombinant Human 60 kDa lysophospholipase (ASPG)

Recombinant Human 60 kDa lysophospholipase (ASPG)

  • 中文名称：
    Recombinant Human 60 kDa lysophospholipase(ASPG),Yeast
  • 货号：
    CSB-YP773026HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human 60 kDa lysophospholipase(ASPG),Yeast
  • 货号：
    CSB-EP773026HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human 60 kDa lysophospholipase(ASPG),Yeast
  • 货号：
    CSB-EP773026HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human 60 kDa lysophospholipase(ASPG),Yeast
  • 货号：
    CSB-BP773026HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human 60 kDa lysophospholipase(ASPG),Yeast
  • 货号：
    CSB-MP773026HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ASPG
  • Uniprot No.：
  • 别名：
    ASPG; C14orf76; 60 kDa lysophospholipase; LysoLP; EC 3.1.1.5; Lysophospholipase-transacylase) [Includes: L-asparaginase; EC 3.5.1.1; L-asparagine amidohydrolase); 1-alkyl-2-acetylglycerophosphocholine esterase; EC 3.1.1.47; Platelet-activating factor acetylhydrolase; PAF acetylhydrolase)]
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-573
  • 氨基酸序列
    MARAVGPERR LLAVYTGGTI GMRSELGVLV PGTGLAAILR TLPMFHDEEH ARARGLSEDT LVLPPASRNQ RILYTVLECQ PLFDSSDMTI AEWVCLAQTI KRHYEQYHGF VVIHGTDTMA FAASMLSFML ENLQKTVILT GAQVPIHALW SDGRENLLGA LLMAGQYVIP EVCLFFQNQL FRGNRATKVD ARRFAAFCSP NLLPLATVGA DITINRELVR KVDGKAGLVV HSSMEQDVGL LRLYPGIPAA LVRAFLQPPL KGVVMETFGS GNGPTKPDLL QELRVATERG LVIVNCTHCL QGAVTTDYAA GMAMAGAGVI SGFDMTSEAA LAKLSYVLGQ PGLSLDVRKE LLTKDLRGEM TPPSVEERRP SLQGNTLGGG VSWLLSLSGS QEADALRNAL VPSLACAAAH AGDVEALQAL VELGSDLGLV DFNGQTPLHA AARGGHTEAV TMLLQRGVDV NTRDTDGFSP LLLAVRGRHP GVIGLLREAG ASLSTQELEE AGTELCRLAY RADLEGLQVW WQAGADLGQP GYDGHSALHV AEAAGNLAVV AFLQSLEGAV GAQAPCPEVL PGV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities. Can catalyze three types of transacylation reactions: (1) acyl transfer from 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to the sn-1(3) positions of glycerol and 2-acylglycerol (sn-1 to -1(3) transfer), (2) acyl transfer from 1-acyl-GPC to the sn-2 positions of 1-acyl-GPC, 1-acyl-sn-glycero-3-phosphoethanolamine (1-acyl-GPE), and other lysophospholipids (sn-1 to -2 transfer) and (3) acyl transfer from 2-acyl-GPC to the sn-1 position of 2-acyl-GPC and 2-acyl-GPE (sn-2 to -1 transfer). Mediates the synthesis of 1-arachidonoyl species of phospholipids by transferring the arachidonoyl residue from 2-arachidonoyl lysophospholipid to the sn-1 position of 2-acyl lysophospholipid.
  • 基因功能参考文献：
    1. To determine the individual contribution of each of the three conserved active-site threonines (threonine triad Thr168, Thr186, Thr219) for the enzyme-activating autocleavage and asparaginase reactions, we prepared the T168S, T186V and T219A/V mutants. PMID: 24768817
    2. Human 60-kDa lysophospholipase contains an N-terminal L-asparaginase domain that is allosterically regulated by L-asparagine. PMID: 24657844
    3. In the cleaved state, Thr168 of L-asparaginase directly activates its hydroxyl group (distance of 2.9 A). PMID: 22861376
    4. The 60kDa lysophospholipase downregulates basal and Sgk1-dependent ENaC activity. PMID: 21063096
  • 蛋白家族：
    Asparaginase 1 family
  • 数据库链接：

    HGNC: 20123

    KEGG: hsa:374569

    STRING: 9606.ENSP00000450040

    UniGene: Hs.146239