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  4. Recombinant Human 5,6-dihydroxyindole-2-carboxylic acid oxidase (TYRP1), partial

Recombinant Human 5,6-dihydroxyindole-2-carboxylic acid oxidase (TYRP1), partial

  • 货号：
    CSB-YP025399HU1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025399HU1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025399HU1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025399HU1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025399HU1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    TYRP1; CAS2; TYRP; TYRRP; 5,6-dihydroxyindole-2-carboxylic acid oxidase; DHICA oxidase; Catalase B; Glycoprotein 75; Melanoma antigen gp75; Tyrosinase-related protein 1; TRP; TRP-1; TRP1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in melanin biosynthesis. Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu(2+) ions, but not in the presence of Zn(2+). May regulate or influence the type of melanin synthesized. Also to a lower extent, capable of hydroxylating tyrosine and producing melanin.
  • 基因功能参考文献：
    1. The rs387907171 SNP in TYRP1 exhibits strong allele frequency differences among islands in Northern Island Melanesia. Its absence from Bougainville, as well as the weak association with decreased hair color, indicates that additional alleles contribute to the blondism phenotype. PMID: 26450459
    2. TYRP1-dependent miR-16 sequestration can also be overcome in vivo by using small oligonucleotides that mask miR-16-binding sites on TYRP1 mRNA. PMID: 28991221
    3. The mRNA of TYRP1 is now found to sequester the tumour suppressor miR-16. PMID: 29087386
    4. DNA sequencing showed that the patient has carried compound heterozygous mutations of the tyrosinase related protein (TYRP1) gene, namely c.1214C>A (p.T405N) and c.1333dupG, which were inherited from his mother and father, respectively. PMID: 28186599
    5. Single nucleotide polymorphisms in TYRP1 gene is associated with multiple primary melanoma. PMID: 25837821
    6. Mutation in TYRP1 is associated with oculocutaneous albinism. PMID: 26252096
    7. Polymorphisms in 3'UTR of TYRP1 mRNA can affect TYRP1 mRNA regulation by miR-155 and its subsequent translation into protein. These SNPs can render TYRP1 mRNA and protein expression nonsusceptible to miR-155 activity PMID: 26068396
    8. These data suggest that UVB-stimulated Ucn1 contributes to TRP1 production via the transcription of both Nurr-1 and Nur77. Ucn1, produced in melanoma cells, acts on melanoma cells themselves in an autocrine manner. PMID: 25240771
    9. These data indicate that galectin-3 is a regulatory component in melanin synthesis affecting the expression of Tyrp-1. PMID: 25054620
    10. p53 regulation by TRP2 is not pervasive in melanoma. PMID: 24475287
    11. Due to mutation in Tyrp1 protein, it became more rigid and might disturb the structural conformation and catalytic function of the structure and might also play a significant role in inducing oculocutaneous albinism type III. PMID: 23862152
    12. in human melanoma HMV-II cells both CRF and Ucn1 regulate TRP1 gene expression via Nurr-1/Nur77 production, independent of pro-opiomelanocortin or alpha-melanocyte-stimulating hormone stimulation. PMID: 23416839
    13. High TYRP1/S100B mRNA expression in lymph node metastases from melanoma patients is associated with unfavourable clinical outcome. PMID: 23519055
    14. We report four Pakistani albinism mutations, including three SLC45A2 alleles and one 22-nucleotide deletion in TYRP1. PMID: 23190901
    15. identifed an arginine-to-cysteine change at a highly conserved residue in TYRP1 as a major determinant of blond hair in Solomon Islanders; this missense mutation predicted to affect catalytic activity of TYRP1 and causes blond hair through a recessive mode of inheritance PMID: 22556244
    16. We used the first ranked gene, tyrosinase-related protein 1 (TYRP1), further measured its expression in the validation population by real-time PCR and found it to be significantly correlated with distant metastasis-free survival. PMID: 22045183
    17. two novel mutations in TYRP1 gene in two Chinese patients with oculocutaneous albinism type 3 PMID: 21739261
    18. Case Report: Report a Japanese girl with oculocutaneous albinism type 3 and novel mutations in TYRP1 gene. PMID: 21996312
    19. This review focuses on TYRP1, a melanosomal protein involved in the pigmentary machinery of the melanocyte and often used as differentiation marker, with a special emphasis on its emerging roles in the malignant melanocyte and melanoma progression. PMID: 21324755
    20. interactions between TRP1-GIPC and GIPC-APPL-AKT provide a potential link between melanogenesis and PI3 kinase signaling PMID: 21291857
    21. Adoptive transfer of TRP1-specific T cells into -expressing recipients produces large confluent patches of vitiligo and ocular damage in TRP1-specific T cell receptor transgenic mice. PMID: 20668223
    22. Tyrp1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. Tyrp1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and melanocyte cell death. PMID: 11775055
    23. DNA sequence variants in the human TYRP1 gene are not associated with inherited pigmentary glaucoma in humans. PMID: 12011806
    24. We have identified the first TYRP1 mutation in non-Africans and have confirmed that TYRP1 mutations are associated with a milder phenotype of oculocutaneous albinism. PMID: 15996218
    25. The presence of a distal Tyrp1 regulatory element, which specifies melanocyte-specific expression, supports the idea that separate regulatory sequences can mediate differential gene expression in melanocytes and RPE. PMID: 16934245
    26. Melanocytes in vitiligo demonstrate reduced ability to withstand oxidative stress due, partly, to a disruption in microphthalmia-associated transcription factor regulation of Tyrp1. PMID: 17071589
    27. We observe strong evidence for positive selection for TYRP1 alleles in Africans and in Asians PMID: 18312627
    28. An eye color variant in TYRP1 was associated with risk of cutaneous melanoma. PMID: 18488027
    29. Mutation of TYRP1 (OCA3) can modify the OCA2 phenotype, resulting in red hair. PMID: 18680187
    30. Both TRP-1 and galectin-1 were highly expressed in normal melanocytes and melanoma. There was no correlation between TRP-1 or galectin-1 expression and survival. PMID: 19287070
    31. TYRP1 rs1408799 is associated with melanoma. PMID: 19384953

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  • 相关疾病：
    Albinism, oculocutaneous, 3 (OCA3)
  • 亚细胞定位：
    Melanosome membrane; Single-pass type I membrane protein. Melanosome.
  • 蛋白家族：
    Tyrosinase family
  • 组织特异性：
    Pigment cells.
  • 数据库链接：

    HGNC: 12450

    OMIM: 115501

    KEGG: hsa:7306

    STRING: 9606.ENSP00000373570

    UniGene: Hs.270279