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  4. Recombinant Human 40S ribosomal protein SA (RPSA)

Recombinant Human 40S ribosomal protein SA (RPSA)

In Stock
  • 货号：
    CSB-EP020485HUb1
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    RPSA
  • Uniprot No.：
  • 别名：
    34/67 kDa laminin receptor; 37 kDa laminin receptor precursor; 37/67 kDa laminin receptor; 37LRP; 40S ribosomal protein SA; 67 kDa laminin receptor; 67LR; Colon carcinoma laminin binding protein; Colon carcinoma laminin-binding protein; LAMBR; Laminin receptor 1; Laminin-binding protein precursor p40; LAMR 1; LamR; LAMR1; LBP; LBP/p40; LRP; LRP/LR; Multidrug resistance associated protein MGr1 Ag; Multidrug resistance associated protein MGr1Ag; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; p40; Ribosomal Protein SA; rpsA; RSSA_HUMAN; SA
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    37.7 kDa
  • 表达区域：
    2-295aa
  • 氨基酸序列
    SGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTATQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTDWS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The recombinant human RPSA protein is encoded by a recombinant DNA that was cloned into the expression vector and then transformed into the E.coli that supports the expression of the gene. The recombinant DNA was constructed by fusing the N-terminal 10xHis tag and C-terminal Myc tag gene to the gene fragment coding for the 2-295aa of the human RPSA protein. After purification, the product is the recombinant human RPSA protein. This recombinant RPSA protein was subjected to the SDS-PAGE determination. Its purity reaches over 90% evaluated by Bandscan software analysis combined with SAS-PAGE. This RPSA protein ran to the molecular weight of about 35-40 kDa under SDS-PAGE condition. It may have applications in cardiovascular research.

    RPSA protein is a ribosomal protein which encoded by RPSA gene in human. RPSA gene consists of seven exons, six of which correspond to the coding sequence. RPSA structure generally is divided in an N-domain (residues 1-209) and a C-domain (residues 210-295). The structure of the N-domain of RPSA is similar to those of prokaryotic RPS2. RPSA is originally discovered by methods of cellular biology. RPSA binds to proteins that are involved in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA.; (Microbial infection) Acts as a receptor for the Adeno-associated viruses 2,3,8 and 9.; (Microbial infection) Acts as a receptor for the Dengue virus.; (Microbial infection) Acts as a receptor for the Sindbis virus.; (Microbial infection) Acts as a receptor for the Venezuelan equine encephalitis virus.; (Microbial infection) Acts as a receptor for the pathogenic prion protein.; (Microbial infection) Acts as a receptor for bacteria.
  • 基因功能参考文献：
    1. Mutations in RPSA exons can affect the translated or untranslated regions and can underlie Isolated congenital asplenia with complete or incomplete penetrance. PMID: 30072435
    2. The binding of laminin-1 to 67LR causes initial signaling through PKA and Epac, which causes the internalization of 67LR, along with signaling enzymes, such as adenylyl cyclase, into early endosomes. This causes sustained signaling for protection against neuronal cell death induced by serum withdrawal. PMID: 29108990
    3. We suggest that PrP(C) and its interactor, LR/37/67 kDa, could be potential therapeutic targets for schwannomas and other Merlin-deficient tumours. PMID: 28692055
    4. Our results suggest that hypoxia-elicited c-Jun/activator protein 1 regulates 37-kDa laminin receptor precursor expression, which modulates migration and invasion of lung adenocarcinoma cells PMID: 28618937
    5. Increased LRP levels correlate with the increased invasive and adhesive potential in early and late stage melanoma cells. PMID: 28118986
    6. A polysaccharide from Pinellia ternata inhibits cell proliferation and metastasis in human cholangiocarcinoma cells by targeting of Cdc42 and 67kDa Laminin Receptor PMID: 27576948
    7. 67LR plays a considerable role in the development of colon cancer multidrug resistance PMID: 26293895
    8. 37LRP regulates the metastasis of glioma cells in vitro and tumor growth in vivo. PMID: 27748570
    9. Higher molecular weight Rpsa requires sumoylation to form. PMID: 26146125
    10. Knock-Down of the 37kDa/67kDa Laminin Receptor LRP/LR Impedes Telomerase Activity PMID: 26545108
    11. High expression level of the laminin receptor is associated with Breast and Oesophageal Cancer. PMID: 26427016
    12. Report discovery of new small molecules inhibiting 67 kDa laminin receptor interaction with laminin and cancer cell invasivness. PMID: 26062445
    13. LR1 contributes to hypoxia-induced migration and invasion of trophoblast cells at least partly by mediating MMP-9 PMID: 25800042
    14. Mutations in the gene RPSA, which encodes ribosomal protein SA, cause more than half of the cases of isolated congenital asplenia. These disease-causing mutations lead to haploinsufficiency of RPSA. Review. PMID: 25840456
    15. analysis of the complex three-way interaction between the non-integrin laminin receptor, galectin-3 and Neisseria meningitidis PMID: 25274119
    16. Studies indicate that the expression of 37/67-kDa immature laminin receptor protein (iLRP)iLRP in the rodent and human fetus is phase specific PMID: 25082063
    17. Suggest role for 67LR/mitogen activated protein kinase/DUSP pathway in influencing glioma cell proliferation. PMID: 25778325
    18. a biological marker in distinguishing squamous cell carcinoma: from adenocarcinoma, neuroendocrine carcinomas, and malignant mesothelioma PMID: 24805133
    19. LRP/LR is a receptor for amyloid-beta42 internalization, mediating its endocytosis and contributing to the cytotoxicity of the neuropeptide. PMID: 24990253
    20. Data suggest thet 67-kDa laminin receptor-dependent protein phosphatase 2A (67LR/PP2A) may be a promising therapeutic target for melanomas. PMID: 25294877
    21. Results indicate that MGr1-Ag/37LRP contributes to laminin-mediated cell adhesion-mediated drug resistance in gastric cancer cells. PMID: 24840404
    22. Mutants in the RPSA gene might be associated with genetic susceptibility to colorectal cancer. PMID: 24460263
    23. LamR interaction with the yeast prion-forming protein, Sup35, was investigated. PMID: 24416454
    24. as a receptor of ECM components, MGr1-Ag/37LRP may activate the downstream signal pathway PI3K/AKT and MAPK/ERK through interaction with phosphorylated FAK. PMID: 24703465
    25. RNA interference-mediated silencing of laminin receptor 1 (LR1) suppresses migration and invasion and down-regulates matrix metalloproteinase (MMP)-2 and MMP-9 in trophoblast cells. PMID: 23729238
    26. findings indicate that 37/67LR regulates proliferation and adhesion in normal intestinal epithelial cells independently of its known association with ribosomal function PMID: 23991217
    27. PEDF causes anti-angiogenic, anti-inflammatory and anti-thrombogenic reactions in myeloma cells through the interaction with LR. PMID: 24342618
    28. LRP/LR is involved in the maintenance of cellular viability in tumorigenic lung and cervix uteri cells through the blockage of apoptosis PMID: 23472084
    29. MGr1-Ag promotes small-cell lung cancer cell invasion and bone metastasis in vitro and in vivo, and that this is partially mediated via the epithelial-mesenchymal transition pathway. PMID: 23588894
    30. This discovery establishes an essential role for RPSA in human spleen development. PMID: 23579497
    31. 67LR functions as a cancer-specific death receptor. In this cell death receptor pathway, cGMP initiated cancer-specific cell death by activating the PKCdelta/acid sphingomyelinase (PKCdelta/ASM) pathway. PMID: 23348740
    32. Identification of novel laminin receptor binding proteins from whole cell extracts. PMID: 22909348
    33. The 67 kD laminin receptor is a novel PED/PEA-15 interacting protein. PED/PEA-15 overexpression increases 67LR-mediated cell adhesion and migration to laminin and extracellular matrix invasion. PMID: 21895963
    34. results suggest that RNF8 and BRCA1 are anchored to the nucleolus through reversible interactions with RPSA PMID: 22814251
    35. the nature of the EGCG-67LR interaction and novel structural insights into the understanding of 67LR-mediated functions of EGCG PMID: 22666419
    36. a high plasticity of RPSA, which could be important for its multiple cellular localizations and functional interactions PMID: 22640394
    37. key structural determinants of the interaction of LamR with laminin-1 PMID: 22290616
    38. Data indicate that high iLR expression was strongly correlated with negativity for CD38 and ZAP-70 expression and mutated IGVH gene status. PMID: 21055809
    39. Decreased LR1 expression in cytotrophoblasts and syncytiotrophoblasts of preeclamptic placentas, which may be independent of disease severity, might have a role in shallow trophoblastic invasion in preeclampsia. PMID: 21391874
    40. The genotypes and allele frequencies of the RPSA polymorphisms showed no significant differences between the controls and sporadic CJD patients. PMID: 21838916
    41. The ability of LAMR to regulate viability is associated with its C-terminal 75 residues. PMID: 21243100
    42. the SA C-terminal domain in the spatial structure of the 40S subunit PMID: 21167900
    43. 67-kDa laminin receptor expression influenced the characteristics of leukemia cells toward an aggressive phenotype and increased the number of granulocyte-macrophage colony-stimulating factor receptors PMID: 21056082
    44. Here, the s identify a laminin binding site on LamR, comprising residues Phe32, Glu35, and Arg155, which are conserved among mammalian species. PMID: 21040730
    45. 67LR induces FasL expression and cytotoxicity against Fas-sensitive Jurkat T cells in human cholangiocarcinoma cells through the phosphorylation of c-Myc on Ser-62 and the subsequent activation of the FasL promoter through the ERK pathway PMID: 20101459
    46. Studies indicate that the 37-kDa/67 kDa laminin receptor is a receptor for the cellular prion protein (PrPc). PMID: 20515747
    47. 67LR promotes the invasive and metastatic ability of the gastric cancer cells through increasing urokinase and MMP 9 expression. PMID: 20491781
    48. TSAd associates with laminin binding protein and mediates T lymphocyte migration during T cell activation PMID: 19561400
    49. Results suggested that the LBP receptor domain interacting with venezuelan equine encephalitis E2 and tick-borne encephalitis virus E viral proteins is located at the C-terminal fragment of the LBP molecule. PMID: 19961413
    50. Tthese results show that 37LRP has some of the biological activities of 67LR, even prior to the conversion event. However, the conversion affects the sites of interaction with both laminin and heparan sulfate. PMID: 19691449

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  • 相关疾病：
    Asplenia, isolated congenital (ICAS)
  • 亚细胞定位：
    Cell membrane. Cytoplasm. Nucleus.
  • 蛋白家族：
    Universal ribosomal protein uS2 family
  • 数据库链接：

    HGNC: 6502

    OMIM: 150370

    KEGG: hsa:3921

    STRING: 9606.ENSP00000346067

    UniGene: Hs.449909