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  4. Recombinant Human 14-3-3 protein epsilon (YWHAE)

Recombinant Human 14-3-3 protein epsilon (YWHAE)

  • 货号：
    CSB-YP026287HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026287HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP026287HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026287HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026287HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    14 3 3 E; 14 3 3 epsilon; 14 3 3E; 14-3-3 protein epsilon; 14-3-3E; 1433E_HUMAN; Epididymis luminal protein 2; FLJ45465; FLJ53559; HEL2; KCIP 1; KCIP1; MDCR; MDS; Mitochondrial import stimulation factor L subunit ; Protein kinase C inhibitor protein1 ; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; epsilon; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; epsilon polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide; YWHAE
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-255
  • 氨基酸序列
    MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV EDENQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.
  • 基因功能参考文献：
    1. results support YWHAE being a risk gene for Major Depressive Disorder in the Han Chinese population. PMID: 28414084
    2. YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells. PMID: 27863420
    3. we report recurrent BCOR exon 16 internal tandem duplications and YWHAE-NUTM2B fusions in half of infantile soft tissue undifferentiated round cell sarcoma and most primitive myxoid mesenchymal tumor of infancy cases, but not in other pediatric sarcomas. PMID: 26945340
    4. For metastatic YWHAE-rearranged HG-ESS, prolonged disease control following diagnosis was seen, with notable responses to anthracycline-based therapy. This emphasizes the need for appropriate molecular testing of uterine mesenchymal malignancies. PMID: 28390819
    5. phosphorus NMR and time-resolved tryptophan fluorescence measurements suggest that 14-3-3zeta interacts with the kinase domain of ASK1 in close proximity to its active site, thus indicating this interaction might block its accessibility and/or affect its conformation. PMID: 27514745
    6. The 14-3-3 family is dysregulated in schizophrenia, perhaps owing to specific regulatory mechanisms; the expression of the 14-3-3 epsilon, theta and zeta isoforms could be useful indicators of disease severity. PMID: 27030512
    7. Studies show that patients with clear cell sarcoma of the kidney (CCSK) and the fusion YWHAE-NUTM2B/E were relatively young, had low tumor volumes, and did not present with stage I disease which fail to identify an explicit clinical phenotype. PMID: 26542179
    8. Data show that the NS3 protein of dengue virus bound to 14-3-3 epsilon protein (14-3-3varepsilon) and prevented translocation of retinoic acid-inducible gene-I protein (RIG-I) to the adaptor MAVS protein and thereby blocked antiviral signaling. PMID: 26998762
    9. Report mutually exclusive BCOR internal tandem duplications and YWHAE-NUTM2 fusions in clear cell sarcoma of kidney. PMID: 27000436
    10. CEP131 is the key regulatory target of MK2 and 14-3-3 in centriolar satellite remodeling. PMID: 26616734
    11. 14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis. PMID: 26208633
    12. DAPK2-induced apoptosis is negatively regulated by Akt and 14-3-3 proteins. PMID: 26047703
    13. These data demonstrate a novel role for HDAC6 in controlling 14-3-3zeta binding activity. PMID: 25770209
    14. YWHAE-FAM22 rearrangement is associated with endometrial stromal sarcomas. PMID: 25244606
    15. Results suggest a specific role for YWHAE single nucleotide polymorphisms in the pathophysiology of schizophrenia. PMID: 25105667
    16. Cleft lip/palate has been found associated with 17p13.3 duplication involving a single candidate gene (YWHAE) in a father and his son. PMID: 23901910
    17. Data show that two isoforms of the 14-3-3 family, 14-3-3epsilon and 14-3-3 gamma, can stabilize Cdt2 independent of each other when overexpressed. PMID: 25154416
    18. These results suggest a possible role for the YWHAE genotype in the early development of the OFC sulcogyral pattern in patients with schizophrenia. PMID: 24561237
    19. data indicate that 14-3-3 is required for Bim expression and that it has an anti-cancer effect under pERK1/2 inhibition in 143B cells PMID: 24536031
    20. for endometrial sarcoma cases with high-grade morphology we recommend to test for YWHAE rearrangements by FISH break-apart PMID: 23599159
    21. YWHAE polymorphisms may be a genetic risk factor for HIV-associated neurocognitive disorder (HAND). PMID: 23982958
    22. Data indicate that 14-3-3 zeta, gamma, epsilon, and tau isoforms but not the sigma protein hydrolyze ATP. PMID: 24269678
    23. Carriers of the YWHAE*C allele of rs3752826 polymorphism have increased risk of suicidal behavior. PMID: 24450199
    24. findings suggest that FAK expression is correlated with and upregulated by 14-3-3epsilon via activation of NFkappaB PMID: 22934705
    25. interaction between NAV2 and 14-3-3epsilon PMID: 24161943
    26. 14-3-3epsilon associates with TAK1 in a phosphorylation-dependent manner to determine the cell fate of Bleomycin-treated HCC cells PMID: 23472066
    27. YWHAE gene duplication is associated with developmental, behavioral and brain abnormalities. PMID: 23813913
    28. 14-3-3epsilon may act as an important regulator in modulating tumor metastasis by promoting epithelial-mesenchymal transition as well as cell migration. PMID: 23483955
    29. The subcellular localization of full-length human MLF1 is 14-3-3epsilon-independent. PMID: 23271436
    30. The ERK/MAPK pathway is involved in the pathogenesis of gastric cancer; RKIP and 14-3-3epsilon exert an opposite effect on this pathway and the cells possibly via both direct and indirect reactions with the elements in this pathway. PMID: 22899242
    31. the -458G>T YWHAE variant contributes to the abnormal myocardial morphogenesis characteristic of LVNC as well as abnormal brain development, and implicate YWHAE as a novel candidate gene in pediatric cardiomyopathies. PMID: 23266643
    32. YWHAE gene contribute to the phenotype of small 17p13.3 chromosomal duplication in Miller-Dieker syndrome PMID: 23035971
    33. results suggest that YWHAE does play a major role in bipolar disorder in the Han Chinese population. PMID: 23140658
    34. These results showed that IgE is produced during malaria, and that the negative correlation between levels of IgE to 14-3-3 epsilon protein and parasitemia in asymptomatic malaria supports a role for these IgE molecules in defense mechanisms. PMID: 22984113
    35. Data obtained, which have never previously been reported, were used to modify the published 14-3-3 crystal structure using molecular modeling. PMID: 22580067
    36. When evaluating high-grade uterine sarcomas, cyclin D1 can be included in the immuno-histochemical panel as an indicator of YWHAE-FAM22endometrial stromal sarcoma. PMID: 22982899
    37. results indicate that the dosage effect of YWHAE varies from severe to very mild structural brain abnormalities, and suggest that the expression of YWHAE is associated with a complex mechanism of neuronal development PMID: 22887762
    38. 14-3-3epsilon is essential for the stable interaction of RIG-I with TRIM25, which facilitates RIG-I ubiquitination and initiation of innate immunity against hepatitis C virus and other pathogenic RNA viruses. PMID: 22607805
    39. Tumors with YWHAE-FAM22 rearrangements constitute a distinct group of endometrial stromal sarcoma (ESS), which is associated with high-grade morphology and aggressive clinical behavior compared to JAZF1 ESS. PMID: 22456610
    40. Collectively, these results identify a novel locus of apoptosomal regulation wherein MAPK signalling promotes Rsk-catalysed Apaf-1 phosphorylation and consequent binding of 14-3-3varepsilon, resulting in decreased cellular responsiveness to cytochrome c. PMID: 22246185
    41. Data present the high-resolution crystal structure of this binding motif [MLF1(29-42)pSer34] in complex with 14-3-3epsilon and analyse the interaction with isothermal titration calorimetry. PMID: 22151054
    42. Fluorescence in situ hybridization analysis demonstrated absolute specificity of YWHAE-FAM22A/B genetic rearrangement for high-grade endometrial stromal sarcoma. PMID: 22223660
    43. YWHAE does not play a major role in schizophrenia, major depressive disorder or bipolar disorder in the Han Chinese populatio PMID: 21184166
    44. Data suggest a regulatory circuitry involving Smad4 dependent up-regulation of KRT23 (directly or indirectly) which in turn modulates the interaction between KRT23 and 14-3-3epsilon leading to a cytoplasmic sequestration of 14-3-3epsilon. PMID: 21492476
    45. Results suggest that ERK and p38 MAPK regulate subcellular localization of Prdx6 by activation of 14-3-3epsilon as a chaperone protein, resulting in its translocation to acidic organelles. PMID: 21346153
    46. Overexpression of 14-3-3epsilon predicts tumour metastasis and poor survival in hepatocellular carcinoma PMID: 21401702
    47. For the first time, we found that TNF-alpha stimulation enhances the interactions between 14-3-3 epsilon and some key components in the mitogen-activated protein kinase (MAPK) signal module which is located at the immediate upstream of NF-kappaB. PMID: 20462248
    48. The 14-3-3 epsilon, zeta and theta may be involved in tumorigenesis of meningioma and be efficient markers for predicting the degree of malignancy in meningioma. PMID: 20388496
    49. Decreased expression of 14-3-3epsilon in contributes to the initiation and progression of laryngeal carcinoma by affecting apoptosis and invasion. PMID: 20565895
    50. the identification of a novel splicing variant of human 14-3-3 epsilon (14-3-3 epsilon sv), derived from a novel exon 1' insertion is reported. PMID: 20417184

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Melanosome.
  • 蛋白家族：
    14-3-3 family
  • 数据库链接：

    HGNC: 12851

    OMIM: 605066

    KEGG: hsa:7531

    STRING: 9606.ENSP00000264335

    UniGene: Hs.513851