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  4. Recombinant Escherichia coli Uncharacterized protein YeaZ (yeaZ)

Recombinant Escherichia coli Uncharacterized protein YeaZ (yeaZ)

  • 货号：
    CSB-YP305160ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP305160ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP305160ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP305160ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP305160ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tsaB
  • Uniprot No.：
  • 别名：
    tsaB; yeaZ; b1807; JW1796; tRNA threonylcarbamoyladenosine biosynthesis protein TsaB; t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-231
  • 氨基酸序列
    MRILAIDTAT EACSVALWND GTVNAHFELC PREHTQRILP MVQDILTTSG TSLTDINALA YGRGPGSFTG VRIGIGIAQG LALGAELPMI GVSTLMTMAQ GAWRKNGATR VLAAIDARMG EVYWAEYQRD ENGIWHGEET EAVLKPEIVH ERMQQLSGEW VTVGTGWQAW PDLGKESGLV LRDGEVLLPA AEDMLPIACQ MFAEGKTVAV EHAEPVYLRN NVAWKKLPGK E
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. In fact, can act as a protease that specifically degrades TsaD in vitro; therefore TsaB may post-translationally regulate cellular pools of TsaD via proteolytic degradation. Does not show sialoglycoprotease activity against glycophorin A.
  • 基因功能参考文献：
    1. in bacteria four proteins (YgjD, YrdC, YjeE, and YeaZ) are both necessary and sufficient for t(6)A biosynthesis in vitro PMID: 22378793
    2. Deliberate repression of the expression of yeaZ results in cells with highly condensed nucleoids, showing that this gene is essential for the viability of E. coli. PMID: 19376873
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    KAE1 / TsaD family, TsaB subfamily
  • 数据库链接：

    KEGG: ecj:JW1796

    STRING: 316385.ECDH10B_1945