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  4. Recombinant Escherichia coli Transcriptional regulatory protein CpxR (cpxR)

Recombinant Escherichia coli Transcriptional regulatory protein CpxR (cpxR)

  • 中文名称：
    Recombinant Escherichia coli Transcriptional regulatory protein CpxR(cpxR),Yeast
  • 货号：
    CSB-YP360074ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Transcriptional regulatory protein CpxR(cpxR),Yeast
  • 货号：
    CSB-EP360074ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Transcriptional regulatory protein CpxR(cpxR),Yeast
  • 货号：
    CSB-EP360074ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Transcriptional regulatory protein CpxR(cpxR),Yeast
  • 货号：
    CSB-BP360074ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Transcriptional regulatory protein CpxR(cpxR),Yeast
  • 货号：
    CSB-MP360074ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    cpxR
  • Uniprot No.：
  • 别名：
    cpxR; yiiA; b3912; JW3883Transcriptional regulatory protein CpxR
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-232
  • 氨基酸序列
    MNKILLVDDD RELTSLLKEL LEMEGFNVIV AHDGEQALDL LDDSIDLLLL DVMMPKKNGI DTLKALRQTH QTPVIMLTAR GSELDRVLGL ELGADDYLPK PFNDRELVAR IRAILRRSHW SEQQQNNDNG SPTLEVDALV LNPGRQEASF DGQTLELTGT EFTLLYLLAQ HLGQVVSREH LSQEVLGKRL TPFDRAIDMH ISNLRRKLPD RKDGHPWFKT LRGRGYLMVS AS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA. Required for efficient binding of stationary phase cells to hydrophobic surfaces, part of the process of biofilm formation. Induced upon cell surface binding, subsequently induces genes it controls (cpxP, dsbA and spy, degP is only partially induced). Binds and activates transcription from the degP promoter; binding is enhanced by phosphorylation. This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E, as well as that of CpxP protein. Other downstream effectors may include SrkA.
  • 基因功能参考文献：
    1. Activation of CpxR upregulates the expression of genes encoding protein folding and chaperone factors and downregulates the expression of a number of envelope proteins/ PMID: 29311237
    2. X-ray structures of the CpxR reveals allosteric switching mechanism, according to which CpxR binding to one CpxA protomer triggers autophosphorylation in the second protomer. PMID: 28552574
    3. findings show that fine-tuning the Cpx stress response is critical to maintain cell wall homeostasis, affecting cell growth, morphology, division, and antibiotic resistance PMID: 26908573
    4. The data lend strong support to the hypothesis that acetyl phosphate has a direct effect on transcription regulation in E. coli via phosphorylation of CpxR, and that RNA polymerase acetylation can modulate this response. PMID: 26790713
    5. Data suggest that the cpxA/cpxR signaling system clearly depends on lipid bilayer properties; increase in anionic surface charge of lipid vesicles results in increased phosphorylation of cpxR. PMID: 25993101
    6. Data indicate that activation of the CpxR/CpxA system stimulates multiple antibiotic resistance (mar) transcription, suggesting a new regulatory circuit that enhances the multidrug resistance cascade. PMID: 25294881
    7. A cpxA mutant lacking its phosphatase activity results in constitutive activation of its cognate response regulator, CpxR, and fosfomycin resistance. PMID: 24163343
    8. overexpression represses transcription of the ung gene (uracil-DNA glycosylase) and ultimately leads to the induction of mutation. PMID: 15576781
    9. findings demonstrate that the Cpx (sensor kinase CpxA and the response regulator CpxR ) response is also involved in the expression of the type IV bundle-forming pili of enteropathogenic E. coli (EPEC). PMID: 15629938
    10. biochemical analysis of the CpxRAP envelope stress system of Escherichia coli PMID: 17259177
    11. This study investigated the role of the Cpx response in enteropathogenic Escherichia coli type III secretion through the locus of enterocyte effacement pathogenicity island expression. PMID: 18227171
    12. The CpxA and CpxR regulated genes that played role in envelope protein misfolding and biofilm formation. PMID: 19103922
    13. MalE mutant causes elevated levels of phosphorylated CpxR and it does not affect the phosphorylation level of CpxA. PMID: 19596441

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  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: ecj:JW3883

    STRING: 316385.ECDH10B_4102