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  4. Recombinant Escherichia coli Single-stranded DNA-binding protein (ssb)

Recombinant Escherichia coli Single-stranded DNA-binding protein (ssb)

  • 货号：
    CSB-YP022706ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022706ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022706ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022706ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022706ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ssb
  • Uniprot No.：
  • 别名：
    ssb; exrB; lexC; b4059; JW4020; Single-stranded DNA-binding protein; SSB; Helix-destabilizing protein
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-178
  • 氨基酸序列
    ASRGVNKVI LVGNLGQDPE VRYMPNGGAV ANITLATSES WRDKATGEMK EQTEWHRVVL FGKLAEVASE YLRKGSQVYI EGQLRTRKWT DQSGQDRYTT EVVVNVGGTM QMLGGRQGGG APAGGNIGGG QPQGGWGQPQ QPQGGNQFSG GAQSRPQQSA PAAPSNEPPM DFDDDIPF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism. Acts as a sliding platform that migrates on DNA via reptation. SSB or its 10 C-terminal amino acids stimulates the ATPase activity of RadD.
  • 基因功能参考文献：
    1. Highly cooperative binding requires the 56 amino acid intrinsically disordered C-terminal linker (IDL) that connects the DNA binding domain with the 9 amino acid C-terminal acidic tip that is involved in SSB binding to other proteins involved in genome maintenance. PMID: 28782560
    2. results indicate that SSB linker mutants are defective for SSB-SSB interactions, and when the IDL is removed a terminal SSB-DNA complex results. PMID: 28078720
    3. a model wherein the IDL is responsible for mediating protein-protein interactions critical to each role. When interactions occur between SSB tetramers, cooperative binding to ssDNA results. When binding occurs between SSB and an interactome partner, storage or loading of that protein onto the DNA takes place. PMID: 28127816
    4. Results support the idea that controlling the balance between SSB binding modes is critical for DNA Pol III holoenzyme stability. PMID: 25329071
    5. this study shows that the amino acid composition and length of the intrinsically disordered linkers affects the ssDNA binding mode preferences of SSB protein. PMID: 25562210
    6. The apparent diffusion coefficient of SSB ranged from 70,000 to 170,000nt(2)/s, which is at least 600 times higher than that determined from diffusion on short ssDNA oligomers, and is within the range reported for protein diffusion on double-stranded DNA. PMID: 24792418
    7. Long-range electrostatic effects from the C-peptide may contribute more to regulating the activity of SSB than any engagement of the C-peptide by the single-stranded DNA-domain. PMID: 24606314
    8. The results identify the binding site of the C-peptide on the OB-domain of the single-stranded DNA binding protein and show that most of the C-domain is highly flexible. PMID: 24288378
    9. s provided evidence that the IntI1 integrase is able to counterbalance the observed effect of SSB on attC site folding. PMID: 24296671
    10. These results indicate that a single SSB tetramer must interact simultaneously with multiple protein partners during some essential roles in genome maintenance. PMID: 24021816
    11. In this study, chemical cross-linking, combined with high-mass matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), is used to determine the stoichiometry of the SSB*ssDNA complex. PMID: 22549990
    12. Steady-state kinetic experiments show that SSB stimulates ExoI activity through effects on both apparent k(cat) and K(m). PMID: 21572106
    13. Study obtained experimental evidence of a reptation mechanism for protein movement along DNA wherein a protein slides via DNA bulge formation and propagation. PMID: 21784244
    14. Mass spectrometric analysis of the chi subunit of DNA polymerase III cross-linked to a C-terminal peptide of SSB reveals that K132 of chi and D172 of SSB are in close contact. PMID: 20972214
    15. single-stranded DNA binding is regulated by the C termini of Escherichia coli single-stranded DNA-binding (SSB) protein PMID: 20360609
    16. SSB overexpression increases the stability of the (CAG)75 repeat array and leads to a loss of the bias towards large deletions for the same array. PMID: 19925793
    17. DNA homopolymers and those lacking 8-oxopurines were less reactive toward DNA-SSB protein cross-links PMID: 15823024
    18. the two major modes of SSB binding to DNA PMID: 17490681
    19. Escherichia coli ssDNA-binding protein SSB limits access of RecA to ssDNA, DprA lowers this barrier. PMID: 17803906
    20. Salt-dependent changes in the SSB protein significantly alter the stability of the protein-ssDNA complex. PMID: 18032436

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  • 数据库链接：

    KEGG: ecj:JW4020

    STRING: 316385.ECDH10B_4248