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  4. Recombinant Escherichia coli Serine/threonine-protein kinase HipA (hipA)

Recombinant Escherichia coli Serine/threonine-protein kinase HipA (hipA)

  • 中文名称：
    大肠杆菌hipA重组蛋白
  • 货号：
    CSB-YP326438ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌hipA重组蛋白
  • 货号：
    CSB-EP326438ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌hipA重组蛋白
  • 货号：
    CSB-EP326438ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌hipA重组蛋白
  • 货号：
    CSB-BP326438ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌hipA重组蛋白
  • 货号：
    CSB-MP326438ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    hipA
  • Uniprot No.：
  • 别名：
    hipA; b1507; JW1500; Serine/threonine-protein kinase toxin HipA; Ser/Thr-protein kinase HipA; EC 2.7.11.1; Toxin HipA
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-440
  • 氨基酸序列
    MPKLVTWMNN QRVGELTKLA NGAHTFKYAP EWLASRYARP LSLSLPLQRG NITSDAVFNF FDNLLPDSPI VRDRIVKRYH AKSRQPFDLL SEIGRDSVGA VTLIPEDETV THPIMAWEKL TEARLEEVLT AYKADIPLGM IREENDFRIS VAGAQEKTAL LRIGNDWCIP KGITPTTHII KLPIGEIRQP NATLDLSQSV DNEYYCLLLA KELGLNVPDA EIIKAGNVRA LAVERFDRRW NAERTVLLRL PQEDMCQTFG LPSSVKYESD GGPGIARIMA FLMGSSEALK DRYDFMKFQV FQWLIGATDG HAKNFSVFIQ AGGSYRLTPF YDIISAFPVL GGTGIHISDL KLAMGLNASK GKKTAIDKIY PRHFLATAKV LRFPEVQMHE ILSDFARMIP AALDNVKTSL PTDFPENVVT AVESNVLRLH GRLSREYGSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Toxic component of a type II toxin-antitoxin (TA) system, first identified by mutations that increase production of persister cells, a fraction of cells that are phenotypic variants not killed by antibiotics, which lead to multidrug tolerance. Persistence may be ultimately due to global remodeling of the persister cell's ribosomes. Phosphorylates Glu-tRNA-ligase (AC P04805, gltX, on 'Ser-239') in vivo. Phosphorylation of GltX prevents it from being charged, leading to an increase in uncharged tRNA(Glu). This induces amino acid starvation and the stringent response via RelA/SpoT and increased (p)ppGpp levels, which inhibits replication, transcription, translation and cell wall synthesis, reducing growth and leading to persistence and multidrug resistance. Once the level of HipA exceeds a threshold cells become dormant, and the length of dormancy is determined by how much HipA levels exceed the threshold. The hipA7 mutation (a double G22S D291A mutation) leads to increased generation of persister cells (cells that survive antibiotic treatment) probably by entering into a dormant state, as well as cold-sensitivity. Wild-type cells produce persisters at a frequency of 10(-6) to 10(-5) whereas hipA7 cells produce about 100-fold more persisters. hipA7 decreases the affinity for antitoxin HipB, leading to increased HipA levels and persistence; depending on the protein level, can be toxic enough to reduce cell growth or even kill cells. Generation of persister cells requires (p)ppGpp as cells lacking relA or relA/spoT generate fewer or no persister cells respectively compared to hipA7. The toxic effect of HipA is neutralized by its cognate antitoxin HipB. Also neutralized by overexpression of gltX. With HipB acts as a corepressor for transcription of the hipBA promoter; binding of HipA-HipB to DNA induces a 70 degree bend. This brings together and dimerizes 2 HipA molecules, which distorts the promoter region, preventing sigma-factor binding; additionally HipA and HipB would physically prevent RNA core polymerase from contacting the -35 promoter box. May play a role in biofilm formation.
  • 基因功能参考文献：
    1. Data show that HipA phosphorylates Ser(239) near the active center of GltX and inhibits aminoacylation. PMID: 24095282
    2. These results suggest that hipA plays a significant role during biofilm development and that extracellular DNA is an important structural component of Escherichia coli biofilms. PMID: 23329678
    3. s show that overexpression of the protein kinase HipA in Escherichia coli triggers growth arrest by activating synthesis of the alarmone guanosine tetraphosphate (ppGpp) by the enzyme RelA, a signal typically associated with amino acid starvation. PMID: 23667235
    4. first validated persister-MDT(multidrug tolerance)gene PMID: 15576765
    5. results suggest that the protein kinase activity of HipA is essential for persister formation. PMID: 17041039
    6. HipBA toxin-antitoxin (TA) system affects frequency of survival during the long-term stationary phase PMID: 19129642
    7. HipA-HipB-DNA structure reveals the HipB-operator binding mechanism; dimeric HipB interacts with 2 HipA molecules to inhibit its kinase activity; these studies suggest mechanisms for HipA-mediated persistence and its neutralization by HipB PMID: 19150849

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  • 蛋白家族：
    HipA Ser/Thr kinase family
  • 数据库链接：

    KEGG: ecj:JW1500

    STRING: 316385.ECDH10B_1638