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  4. Recombinant Escherichia coli Septum site-determining protein MinD (minD)

Recombinant Escherichia coli Septum site-determining protein MinD (minD)

  • 中文名称：
    大肠杆菌minD重组蛋白
  • 货号：
    CSB-YP360190ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌minD重组蛋白
  • 货号：
    CSB-EP360190ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌minD重组蛋白
  • 货号：
    CSB-EP360190ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌minD重组蛋白
  • 货号：
    CSB-BP360190ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌minD重组蛋白
  • 货号：
    CSB-MP360190ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    minD
  • Uniprot No.：
  • 别名：
    minD; b1175; JW1164; Septum site-determining protein MinD; Cell division inhibitor MinD
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-270
  • 氨基酸序列
    ARIIVVTSG KGGVGKTTSS AAIATGLAQK GKKTVVIDFD IGLRNLDLIM GCERRVVYDF VNVIQGDATL NQALIKDKRT ENLYILPASQ TRDKDALTRE GVAKVLDDLK AMDFEFIVCD SPAGIETGAL MALYFADEAI ITTNPEVSSV RDSDRILGIL ASKSRRAENG EEPIKEHLLL TRYNPGRVSR GDMLSMEDVL EILRIKLVGV IPEDQSVLRA SNQGEPVILD INADAGKAYA DTVERLLGEE RPFRFIEEEK KGFLKRLFGG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATPase required for the correct placement of the division site. Cell division inhibitors MinC and MinD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings.
  • 基因功能参考文献：
    1. a membrane anchored MinC/MinD complex is targeted to the Z ring through the conserved carboxy tail of FtsZ leading to breakage of FtsZ filaments. PMID: 26268537
    2. The s observed regular end-to-end oscillation over very short distances along the long axes of minicells, supporting the importance of geometry in MinD localization. PMID: 20543068
    3. average ATP consumption rate per unit length, which is proportional to the ATP consumption rate per MinD, increases with cell length until the transition from stochastic switching to regular oscillations and then remains roughly constant. PMID: 20308588
    4. there are rapid and substantial increases in the average MinD oscillation periods in the presence of any of the polyvalent cations Ca++, Mg++, protamine and gentamicin PMID: 19789705
    5. ATPase activity of MinD activated by positioning of MinE binding site on MinD surface during division site selection. PMID: 15458408
    6. lys 11 required for interaction of MinD with membrane, MinC, MinE & itself; the 3 residues in helix 7 that interact with lys 11 not required for binding to the membrane, activation of MinC or binding MinE but are required for MinE to stimulate MinD ATPase PMID: 15629934
    7. the Min proteins are capable of establishing an axis of oscillation that is the initial step in establishment of polarity in spherical cells PMID: 16262780
    8. it was observed that the oscillation period of MinD within rod-like and filamentous cells of Escherichia coli varied by a factor of 4 in the temperature range from 20 degrees C to 40 degrees C PMID: 16936014
    9. In vivo measurements of the mobility of MinD and MinE using fluorescence correlation spectroscopy shows 2 distinct timescales; the diffusion constant of cytoplasmic MinD to be approximately 16 microm(2) s(-1). PMID: 17200601
    10. Min proteins undergo a periodic pole-to-pole oscillation that involves polymerization and ATPase activity of MinD under the controlling influence of MinE. PMID: 17483175
    11. Reports show that both MinD and FtsZ readily assembled on intracytoplasmic membrane surfaces, and this contributes significantly to the lethal division block seen in all shape mutants under nonpermissive conditions. PMID: 17993535
    12. Overproduction of MinC in the presence of MinD inhibits assembly of Z rings. PMID: 19415799

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  • 亚细胞定位：
    Cell inner membrane; Peripheral membrane protein.
  • 蛋白家族：
    ParA family, MinD subfamily
  • 数据库链接：

    KEGG: ecj:JW1164

    STRING: 316385.ECDH10B_1228