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  4. Recombinant Escherichia coli Sec-independent protein translocase protein TatB (tatB), partial

Recombinant Escherichia coli Sec-independent protein translocase protein TatB (tatB), partial

  • 货号：
    CSB-YP301662ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP301662ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP301662ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP301662ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP301662ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tatB
  • Uniprot No.：
  • 别名：
    tatB; mttA2; ysgB; b3838; JW5580; Sec-independent protein translocase protein TatB
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Together with TatC, TatB is part of a receptor directly interacting with Tat signal peptides. TatB may form an oligomeric binding site that transiently accommodates folded Tat precursor proteins before their translocation.
  • 基因功能参考文献：
    1. It has been concluded that Tat signal peptides play roles in substrate targeting and in triggering assembly of the active TatABC translocase. PMID: 28223511
    2. In the TatBC receptor complex the transmembrane helix of each TatB molecule is sandwiched between two TatC molecules, with one of the inter-subunit interfaces incorporating a functionally important cluster of interacting polar residues. Unexpectedly, the s find that TatA also associates with TatC at the polar cluster site. PMID: 27914200
    3. In vitro cross-linking of residues located in the periplasmically-oriented part of the TatBC receptor to TorA[KQ]-30aa-MalE reporter variants harboring a more hydrophobic h-region in their signal peptides confirmed that unlike in TorA[KQ]-30aa-MalE with an unaltered h-region, the mutated reporters moved deep into the TatBC-binding cavity. PMID: 28515319
    4. Twin-arginine translocation (Tat) system TatA exhibits a uniform distribution throughout the inner membrane and that altering the expression of TatBC shows a uncharacterised distribution of TatA in the inner membrane. PMID: 28280110
    5. we confirm the evidence of interactions between TatC fragments and DmsD chaperone. Using a peptide overlapping array, it was shown that the different NarJ subfamily REMPs interact with different regions of the TatB cytoplasmic domains PMID: 26826271
    6. Distributed TatA thus may serve to accompany membrane-interacting Tat substrates to the few TatBC spots in the cells. PMID: 25774531
    7. The data thus suggest that mature protein domains are translocated through a TatA and TaTB environment. PMID: 25003386
    8. A physical interaction between TatC helix 5 and the TatB transmembrane helix was confirmed by the formation of a site-specific disulphide bond between TatC M205C and TatB L9C variants. PMID: 22742417
    9. The transmembrane helix of TatA makes contacts with TatC and precursors recognized by the TatBC complex PMID: 22041896
    10. data are consistent with TatB forming an oligomeric binding site that transiently accommodates folded Tat precursors PMID: 20926683
    11. findigs show the membrane extrinsic domain of TatB forms parallel contacts with at least one other TatB protein; truncation of the C-terminal two thirds of TatB still allows complex formation with TatC, although protein transport is severely compromised PMID: 21237157
    12. Data show that homo- and hetero-oligomeric complexes of the TatA, TatB and TatC integral membrane proteins were consistent with the current model of translocase assembly. PMID: 20169075
    13. the TatABC complex of the twin-arginine translocase in Escherichia coli has specific roles in assembly and stability PMID: 15571732
    14. the data indicate the presence of an additional form of Tat complex containing TatA and TatB, but not TatC PMID: 15663945
    15. Since TatC is invariably complexed with TatB in wild-type complexes, the combined data demonstrate that TatABC complexes are uniformly distributed throughout the E. coli plasma membrane. PMID: 15728576
    16. Data show that the integrity of the TatA TM span is vital for Tat activity, whereas that of TatB can accommodate large-scale distortions and only substitutions of P48 and F94 in TatC blocks activity. PMID: 15853811
    17. analysis of substrate specificity of the TatB and TatC translocases with the consensus motif of a Tat signal peptide PMID: 17229735
    18. tatABC and tatAC expression has a role in the formation of interacting cytoplasmic TatA tubes in Escherichia coli PMID: 18644791

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  • 亚细胞定位：
    Cell inner membrane; Single-pass membrane protein. Note=Localizes at the cell poles.
  • 蛋白家族：
    TatB family
  • 数据库链接：

    KEGG: ecj:JW5580

    STRING: 316385.ECDH10B_4028