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  4. Recombinant Escherichia coli Ribonucleoside-diphosphate reductase 2 subunit beta (nrdF)

Recombinant Escherichia coli Ribonucleoside-diphosphate reductase 2 subunit beta (nrdF)

  • 中文名称：
    大肠杆菌nrdF重组蛋白
  • 货号：
    CSB-YP336265ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌nrdF重组蛋白
  • 货号：
    CSB-EP336265ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌nrdF重组蛋白
  • 货号：
    CSB-EP336265ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌nrdF重组蛋白
  • 货号：
    CSB-BP336265ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌nrdF重组蛋白
  • 货号：
    CSB-MP336265ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    nrdF
  • Uniprot No.：
  • 别名：
    nrdF; ygaD; b2676; JW2651; Ribonucleoside-diphosphate reductase 2 subunit beta; EC 1.17.4.1; R2F protein; Ribonucleotide reductase 2
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-319
  • 氨基酸序列
    MKLSRISAIN WNKISDDKDL EVWNRLTSNF WLPEKVPLSN DIPAWQTLTV VEQQLTMRVF TGLTLLDTLQ NVIGAPSLMP DALTPHEEAV LSNISFMEAV HARSYSSIFS TLCQTKDVDA AYAWSEENAP LQRKAQIIQQ HYRGDDPLKK KIASVFLESF LFYSGFWLPM YFSSRGKLTN TADLIRLIIR DEAVHGYYIG YKYQKNMEKI SLGQREELKS FAFDLLLELY DNELQYTDEL YAETPWADDV KAFLCYNANK ALMNLGYEPL FPAEMAEVNP AILAALSPNA DENHDFFSGS GSSYVMGKAV ETEDEDWNF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis.
  • 基因功能参考文献：
    1. Thus NrdEF supports DNA replication when iron is unavailable to activate the housekeeping NrdAB enzyme. PMID: 21338418
    2. results strongly suggest that E. coli NrdF is an obligate manganese protein in vivo and that the Mn(III)(2)-Y(*) cofactor assembly pathway PMID: 21250660
    3. crystal structure of Mn(II)2-NrdF and Fe(II)2-NrdF reveal different coordination environments; in structures of Mn(II)2-NrdF in complex with reduced and oxidized NrdI, a continuous channel connects NrdI flavin cofactor to the NrdF Mn(II)2 active site PMID: 20688982
    4. Functional long-range proton-coupled electron transfer in ribonucleoside diphosphate reductase occurs via an optimized pathway of amino acids spanning >35 A across the alpha:beta interface. PMID: 19916558
  • 蛋白家族：
    Ribonucleoside diphosphate reductase small chain family
  • 数据库链接：

    KEGG: ecj:JW2651

    STRING: 316385.ECDH10B_2843