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  4. Recombinant Escherichia coli Phosphate regulon transcriptional regulatory protein phoB (phoB)

Recombinant Escherichia coli Phosphate regulon transcriptional regulatory protein phoB (phoB)

  • 中文名称：
    大肠杆菌phoB重组蛋白
  • 货号：
    CSB-YP360274ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌phoB重组蛋白
  • 货号：
    CSB-EP360274ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌phoB重组蛋白
  • 货号：
    CSB-EP360274ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌phoB重组蛋白
  • 货号：
    CSB-BP360274ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌phoB重组蛋白
  • 货号：
    CSB-MP360274ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    phoB
  • Uniprot No.：
  • 别名：
    phoB; b0399; JW0389; Phosphate regulon transcriptional regulatory protein PhoB
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-229
  • 氨基酸序列
    MARRILVVED EAPIREMVCF VLEQNGFQPV EAEDYDSAVN QLNEPWPDLI LLDWMLPGGS GIQFIKHLKR ESMTRDIPVV MLTARGEEED RVRGLETGAD DYITKPFSPK ELVARIKAVM RRISPMAVEE VIEMQGLSLD PTSHRVMAGE EPLEMGPTEF KLLHFFMTHP ERVYSREQLL NHVWGTNVYV EDRTVDVHIR RLRKALEPGG HDRMVQTVRG TGYRFSTRF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.
  • 基因功能参考文献：
    1. The results point to a model showing that PhoB is a component in the signal cascade regulating biofilm formation triggered by fluctuations of Polyphosphate levels in E. coli cells during stationary phase. PMID: 27023099
    2. The expression timing of the core set of PhoB-regulated genes correlates well with the binding affinity of phosphorylated PhoB~P to individual promoters. PMID: 26015501
    3. a combination of single-molecule and ensemble methods was used to investigate the binding mechanism and the impact of dimerization on the protein-DNA complex stability of PhoB(DBD) PMID: 24199636
    4. Data indicate that dimerization constants for the PhoB heterodimer and homodimer (two phosphorylated monomers) were similar, but the rate constant for heterodimer phosphorylation was ~10-fold higher than for the monomer. PMID: 23760278
    5. sigma(4) domain of RpoD achieves promoter recognition through the N-terminal portion of its DNA recognition helix. Unexpectedly, the same recognition helix contacts the transactivation loop and helices alpha2 and alpha3 of PhoB. PMID: 21829166
    6. Crystallography and solution NMR data for the receiver domain of PhoB which show distinct 2-fold symmetric dimers in the inactive and active states. PMID: 16154092
    7. analysis of cofactor-induced pre-active conformation in PhoB PMID: 16929106
    8. PhoB has a role as a global regulator in Escherichia coli gene expression. PMID: 17020555
    9. phosphorylation promotes dimerization via the alpha 4-beta 5-alpha 5 face, which enhances DNA binding and thus the ability of PhoB to regulate transcription. PMID: 19371748

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  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: ecj:JW0389

    STRING: 316385.ECDH10B_0355