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  4. Recombinant Escherichia coli Multidrug transporter MdfA (mdfA), partial

Recombinant Escherichia coli Multidrug transporter MdfA (mdfA), partial

  • 中文名称：
    大肠杆菌mdfA重组蛋白
  • 货号：
    CSB-YP365221ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌mdfA重组蛋白
  • 货号：
    CSB-EP365221ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌mdfA重组蛋白
  • 货号：
    CSB-EP365221ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌mdfA重组蛋白
  • 货号：
    CSB-BP365221ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌mdfA重组蛋白
  • 货号：
    CSB-MP365221ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    mdfA
  • Uniprot No.：
  • 别名：
    mdfA; cmlA; cmr; b0842; JW0826; Multidrug transporter MdfA; Chloramphenicol resistance pump Cmr
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Efflux pump driven by the proton motive force. Confers resistance to a broad spectrum of chemically unrelated drugs. Confers resistance to a diverse group of cationic or zwitterionic lipophilic compounds such as ethidium bromide, tetraphenylphosphonium, rhodamine, daunomycin, benzalkonium, rifampicin, tetracycline, puromycin, and to chemically unrelated, clinically important antibiotics such as chloramphenicol, erythromycin, and certain aminoglycosides and fluoroquinolones. Overexpression results in isopropyl-beta-D-thiogalactopyranoside (IPTG) exclusion and spectinomycin sensitivity. Transport of neutral substrates is electrogenic, whereas transport of cationic substrates is electroneutral. In addition to its role in multidrug resistance, confers extreme alkaline pH resistance, allowing the growth under conditions that are close to those used normally by alkaliphiles. This activity requires Na(+) or K(+).
  • 基因功能参考文献：
    1. MdfA has exceptional promiscuous capabilities not only in its interaction with a plethora of chemically dissimilar compounds, but also concerning the various faces of its modes of energy consumption. [review] PMID: 28951231
    2. Crystallization and X-ray diffraction analysis of the multidrug-resistance transporter MdfA from Escherichia coli has been reported. PMID: 28695852
    3. The MdfA structure consists of four pseudo-symmetrical, three-helix repeats, consistent with the typical structure of an MFS transporter. PMID: 26238402
    4. The mdfA gene can function as transporter responsible for multidrug resistance, and also mediated the synergistic function with its surrounding genes in conjugative plasmids. PMID: 24684286
    5. GlpG is able to recognize and cleave truncated forms of MdfA but not the intact protein. PMID: 19919105
    6. results show that MdfA confers extreme alkaline pH resistance and allows the growth of transformed cells under conditions that are close to those used normally by alkaliphiles (up to pH 10) by maintaining a physiological internal pH PMID: 15371593
    7. results suggest that in the absence of specific chloramphenicol selection pressure, the cmlA gene is maintained by virtue of gene linkage to genes encoding resistance to antimicrobials that are currently approved for use in food animals PMID: 15668031
    8. Arg112 is important for multidrug transport by MdfA. PMID: 16274234
    9. properties of MdfA including its multidrug recognition capacity, proton recognition determinants, aspects of energy utilization, and physiological role [review] PMID: 17114895
    10. the Mdr-binding pocket of MdfA is conformationally sensitive PMID: 19808670
    11. In this study, we got an evidence that MdfA is a chloramphenicol/H+ antiporter. PMID: 9644262

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  • 亚细胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Major facilitator superfamily, MdfA family
  • 数据库链接：

    KEGG: ag:CAA69997

    STRING: 316385.ECDH10B_0911