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  4. Recombinant Escherichia coli FeS cluster assembly protein sufB (sufB)

Recombinant Escherichia coli FeS cluster assembly protein sufB (sufB)

  • 货号：
    CSB-YP301237ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP301237ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP301237ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP301237ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP301237ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    sufB
  • Uniprot No.：
  • 别名：
    sufB; ynhE; b1683; JW5273; FeS cluster assembly protein SufB
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-495
  • 氨基酸序列
    MSRNTEATDD VKTWTGGPLN YKEGFFTQLA TDELAKGINE EVVRAISAKR NEPEWMLEFR LNAYRAWLEM EEPHWLKAHY DKLNYQDYSY YSAPSCGNCD DTCASEPGAV QQTGANAFLS KEVEAAFEQL GVPVREGKEV AVDAIFDSVS VATTYREKLA EQGIIFCSFG EAIHDHPELV RKYLGTVVPG NDNFFAALNA AVASDGTFIY VPKGVRCPME LSTYFRINAE KTGQFERTIL VADEDSYVSY IEGCSAPVRD SYQLHAAVVE VIIHKNAEVK YSTVQNWFPG DNNTGGILNF VTKRALCEGE NSKMSWTQSE TGSAITWKYP SCILRGDNSI GEFYSVALTS GHQQADTGTK MIHIGKNTKS TIISKGISAG HSQNSYRGLV KIMPTATNAR NFTQCDSMLI GANCGAHTFP YVECRNNSAQ LEHEATTSRI GEDQLFYCLQ RGISEEDAIS MIVNGFCKDV FSELPLEFAV EAQKLLAISL EHSVG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The SufBCD complex acts synergistically with SufE to stimulate the cysteine desulfurase activity of SufS. The SufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake from extracellular iron chelators under iron limitation.
  • 基因功能参考文献：
    1. Studies identified SufE-SufS and SufE-SufB interactions. PMID: 20936830
    2. SufB homolog AtNAP1 can complement SufB deficiency in Escherichia coli during oxidative stress. AtNAP1 represents an atypical plastidic SufB-like protein important for Fe-S cluster assembly and for regulating iron homeostasis in Arabidopsis. PMID: 15611066
  • 蛋白家族：
    UPF0051 (ycf24) family
  • 数据库链接：

    KEGG: ecj:JW5273

    STRING: 316385.ECDH10B_1817