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  4. Recombinant Escherichia coli Carbon storage regulator (csrA)

Recombinant Escherichia coli Carbon storage regulator (csrA)

  • 货号：
    CSB-YP304875ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP304875ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP304875ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP304875ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP304875ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    csrA
  • Uniprot No.：
  • 别名：
    csrA; zfiA; b2696; JW2666Carbon storage regulator; Translational dual regulator CsrA
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-61
  • 氨基酸序列
    MLILTRRVGE TLMIGDEVTV TVLGVKGNQV RIGVNAPKEV SVHREEIYQR IQAEKSQQSS Y
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A key translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability, initially identified for its effects on central carbon metabolism. Mediates global changes in gene expression, shifting from rapid growth to stress survival by linking envelope stress, the stringent response and the catabolite repression systems. Binds to the 5'-UTR of mRNA to repress or activate translation; 2 binding sites on the homodimer can bridge 2 sites within target RNA. Exerts reciprocal effects on enzymes of gluconeogenesis and glycogen biosynthesis versus those of glycolysis. Negatively effects glycogen biosynthesis, gluconeogenesis, alters cell size and surface properties. Activates regulates expression of glycolysis genes. Represses biofilm formation. Regulates glycogen synthesis under both aerobic and anaerobic conditions; overexpression strongly inhibits glycogen accumulation. Binds to 4 sites in its own promoter, including the Shine-Dalgarno sequence, repressing its own translation; mutating the binding-sites decreases repression. Indirectly activates transcription from 1 of its 5 promoters, which is responsible for increased expression during stationary phase. Binds to at least 720 transcripts in strain K12 / CF7789, many of which are also part of the stringent response, including relA, spoT and dksA; slightly represses RelA and slightly activates DskA translation. Binds to and represses the ECF sigma factor rpoE promoter. Accelerates the degradation of glgC gene transcripts; overexpression further decreases glgC transcripts. Binds 2 sites in the glgC mRNA leader, 1 of which overlaps the Shine-Dalgarno sequence, preventing ribosome-binding and thus destabilizing the mRNA. Acts to inhibit interaction between the CcdB (also known as LetD) protein and the A subunit of DNA gyrase. Required to activate motility and flagellum biosynthesis through the post-transcriptional activation of flhDC expression by binding to and stabilizing the flhDC message. Represses translation of iraD mRNA via translational coupling to an upstream open reading frame. Binds to mRNA and reduces levels of probable diguanylate cyclases dgcT and dgcZ.; Binds to and is sequestered by non-coding small RNAs (sRNA) CsrB and CsrC which antagonize the activity of CsrA. The consensus RNA-binding site is CAGGA(U/A/C)G which is located in probable hairpin loops. There are 18 sites in CsrB, which cooperatively binds about 18 copies of CsrA. CsrC has 9 sites, and cooperatively binds multiple copies of CsrA. Indirectly activates expression of CsrB and CsrC, both dependently and independently of the BarA-UvrY two-component system. ppGpp activates transcription of CsrA-antagonistic small RNAs CsrB and CsrC, which downregulates CsrA's action on translation during the stringent response.
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    CsrA/RsmA family
  • 数据库链接：

    KEGG: ecj:JW2666

    STRING: 316385.ECDH10B_2864